{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T13:20:36Z","timestamp":1784035236858,"version":"3.55.0"},"update-to":[{"DOI":"10.1371\/journal.pntd.0008966","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2021,1,5]],"date-time":"2021-01-05T00:00:00Z","timestamp":1609804800000}}],"reference-count":64,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2020,12,21]],"date-time":"2020-12-21T00:00:00Z","timestamp":1608508800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>\n                    Larvae of the cestodes\n                    <jats:italic>Taenia solium<\/jats:italic>\n                    and\n                    <jats:italic>Taenia crassiceps<\/jats:italic>\n                    infect the central nervous system of humans.\n                    <jats:italic>Taenia solium<\/jats:italic>\n                    larvae in the brain cause neurocysticercosis, the leading cause of adult-acquired epilepsy worldwide. Relatively little is understood about how cestode-derived products modulate host neural and immune signalling. Acetylcholinesterases, a class of enzyme that breaks down acetylcholine, are produced by a host of parasitic worms to aid their survival in the host. Acetylcholine is an important signalling molecule in both the human nervous and immune systems, with powerful modulatory effects on the excitability of cortical networks. Therefore, it is important to establish whether cestode derived acetylcholinesterases may alter host neuronal cholinergic signalling. Here we make use of multiple techniques to profile acetylcholinesterase activity in different extracts of both\n                    <jats:italic>Taenia crassiceps<\/jats:italic>\n                    and\n                    <jats:italic>Taenia solium<\/jats:italic>\n                    larvae. We find that the larvae of both species contain substantial acetylcholinesterase activity. However, acetylcholinesterase activity is lower in\n                    <jats:italic>Taenia solium<\/jats:italic>\n                    as compared to\n                    <jats:italic>Taenia crassiceps<\/jats:italic>\n                    larvae. Further, whilst we observed acetylcholinesterase activity in all fractions of\n                    <jats:italic>Taenia crassiceps<\/jats:italic>\n                    larvae, including on the membrane surface and in the excreted\/secreted extracts, we could not identify acetylcholinesterases on the membrane surface or in the excreted\/secreted extracts of\n                    <jats:italic>Taenia solium<\/jats:italic>\n                    larvae. Bioinformatic analysis revealed conservation of the functional protein domains in the\n                    <jats:italic>Taenia solium<\/jats:italic>\n                    acetylcholinesterases, when compared to the homologous human sequence. Finally, using whole-cell patch clamp recordings in rat hippocampal brain slice cultures, we demonstrate that\n                    <jats:italic>Taenia<\/jats:italic>\n                    larval derived acetylcholinesterases can break down acetylcholine at a concentration which induces changes in neuronal signalling. Together, these findings highlight the possibility that\n                    <jats:italic>Taenia<\/jats:italic>\n                    larval acetylcholinesterases can interfere with cholinergic signalling in the host, potentially contributing to pathogenesis in neurocysticercosis.\n                  <\/jats:p>","DOI":"10.1371\/journal.pntd.0008966","type":"journal-article","created":{"date-parts":[[2020,12,21]],"date-time":"2020-12-21T14:42:42Z","timestamp":1608561762000},"page":"e0008966","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":6,"title":["Taenia larvae possess distinct acetylcholinesterase profiles with implications for host cholinergic signalling"],"prefix":"10.1371","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7117-8979","authenticated-orcid":true,"given":"Anja","family":"de 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