{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T09:16:50Z","timestamp":1793611010122,"version":"4.5.0"},"update-to":[{"DOI":"10.1371\/journal.pntd.0009179","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2021,2,26]],"date-time":"2021-02-26T00:00:00Z","timestamp":1614297600000}}],"reference-count":58,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2021,2,16]],"date-time":"2021-02-16T00:00:00Z","timestamp":1613433600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["1132412"],"award-info":[{"award-number":["1132412"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["1118640"],"award-info":[{"award-number":["1118640"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["108508"],"award-info":[{"award-number":["108508"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>\n                    The endosymbiotic bacterium\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    shows viral blocking in its mosquito host, leading to its use in arboviral disease control. Releases with\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    strains\n                    <jats:italic>w<\/jats:italic>\n                    Mel and\n                    <jats:italic>w<\/jats:italic>\n                    AlbB infecting\n                    <jats:italic>Aedes aegypti<\/jats:italic>\n                    have taken place in several countries. Mosquito egg survival is a key factor influencing population persistence and this trait is also important when eggs are stored prior to releases. We therefore tested the viability of mosquitoes derived from\n                    <jats:italic>Wolbachia w<\/jats:italic>\n                    Mel and\n                    <jats:italic>w<\/jats:italic>\n                    AlbB-infected as well as uninfected eggs after long-term storage under diurnal temperature cycles of 11\u201319\u00b0C and 22\u201330\u00b0C. Eggs stored at 11\u201319\u00b0C had higher hatch proportions than those stored at 22\u201330\u00b0C. Adult\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    density declined when they emerged from eggs stored for longer, which was associated with incomplete cytoplasmic incompatibility (CI) when\n                    <jats:italic>w<\/jats:italic>\n                    Mel-infected males were crossed with uninfected females. Females from stored eggs at both temperatures continued to show perfect maternal transmission of\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    , but storage reduced the fecundity of both\n                    <jats:italic>w<\/jats:italic>\n                    Mel and\n                    <jats:italic>w<\/jats:italic>\n                    AlbB-infected females relative to uninfected mosquitoes. Furthermore, we found a very strong negative impact of the\n                    <jats:italic>w<\/jats:italic>\n                    AlbB infection on the fertility of females stored at 22\u201330\u00b0C, with almost 80% of females hatching after 11 weeks of storage being infertile. Our findings provide guidance for storing\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    -infected\n                    <jats:italic>A<\/jats:italic>\n                    .\n                    <jats:italic>aegypti<\/jats:italic>\n                    eggs to ensure high fitness adult mosquitoes for release. Importantly, they also highlight the likely impact of egg quiescence on the population dynamics of\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    -infected populations in the field, and the potential for\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    to suppress mosquito populations through cumulative fitness costs across warm and dry periods, with expected effects on dengue transmission.\n                  <\/jats:p>","DOI":"10.1371\/journal.pntd.0009179","type":"journal-article","created":{"date-parts":[[2021,2,16]],"date-time":"2021-02-16T17:34:53Z","timestamp":1613496893000},"page":"e0009179","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":71,"title":["Infertility and fecundity loss of Wolbachia-infected Aedes aegypti hatched from quiescent eggs is expected to alter invasion 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