{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T18:27:31Z","timestamp":1787855251321,"version":"build-2784847793"},"reference-count":37,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2021,3,23]],"date-time":"2021-03-23T00:00:00Z","timestamp":1616457600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Institute of Health, Malaysia","award":["NIH-18-12-39653"],"award-info":[{"award-number":["NIH-18-12-39653"]}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      In Malaysia, dengue remains a top priority disease and usage of insecticides is the main method for dengue vector control. Limited baseline insecticide resistance data in dengue hotspots has prompted us to conduct this study. The present study reports the use of a map on the insecticide susceptibility status of\n                      <jats:italic>Aedes aegypti<\/jats:italic>\n                      and\n                      <jats:italic>Aedes albopictus<\/jats:italic>\n                      to provide a quick visualization and overview of the distribution of insecticide resistance.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Method and results<\/jats:title>\n                    <jats:p>\n                      The insecticide resistance status of\n                      <jats:italic>Aedes<\/jats:italic>\n                      populations collected from 24 dengue hotspot areas from the period of December 2018 until June 2019 was proactively monitored using the World Health Organization standard protocol for adult and larval susceptibility testing was conducted, together with elucidation of the mechanisms involved in observed resistance. For resistance monitoring, susceptibility to three adulticides (permethrin, deltamethrin, and malathion) was tested, as well as susceptibility to the larvicide, temephos. Data showed significant resistance to both deltamethrin and permethrin (pyrethroid insecticides), and to malathion (organophosphate insecticide) in all sampled\n                      <jats:italic>Aedes aegypti<\/jats:italic>\n                      populations, while variable resistance patterns were found in the sampled\n                      <jats:italic>Aedes albopictus<\/jats:italic>\n                      populations. Temephos resistance was observed when larvae were tested using the diagnostic dosage of 0.012mg\/L but not at the operational dosage of 1mg\/L for both species.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The present study highlights evidence of a potential threat to the effectiveness of insecticides currently used in dengue vector control, and the urgent requirement for insecticide resistance management to be integrated into the National Dengue Control Program.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pntd.0009205","type":"journal-article","created":{"date-parts":[[2021,3,23]],"date-time":"2021-03-23T13:23:35Z","timestamp":1616505815000},"page":"e0009205","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":22,"title":["Insecticide resistance in dengue vectors from hotspots in Selangor, Malaysia"],"prefix":"10.1371","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2009-1547","authenticated-orcid":true,"given":"Rosilawati","family":"Rasli","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2786-7195","authenticated-orcid":true,"given":"Yoon Ling","family":"Cheong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7052-5118","authenticated-orcid":true,"given":"M. Khairuddin","family":"Che Ibrahim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siti Futri","family":"Farahininajua Fikri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1716-4645","authenticated-orcid":true,"given":"Rusydi Najmuddin","family":"Norzali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6866-3888","authenticated-orcid":true,"given":"Nur Ayuni","family":"Nazarudin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4364-8777","authenticated-orcid":true,"given":"Nur Fadillah","family":"Hamdan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9547-1830","authenticated-orcid":true,"given":"Khairul Asuad","family":"Muhamed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8076-2448","authenticated-orcid":true,"given":"Afiq Ahnaf","family":"Hafisool","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruziyatul Aznieda","family":"Azmi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harith Aswad","family":"Ismail","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roziah","family":"Ali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0135-9720","authenticated-orcid":true,"given":"Nurulhusna","family":"Ab Hamid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohd Zainuldin","family":"Taib","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Topek","family":"Omar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nazni","family":"Wasi Ahmad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5391-7873","authenticated-orcid":true,"given":"Han Lim","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,3,23]]},"reference":[{"key":"pntd.0009205.ref001","first-page":"1","author":"World Health Organization (WHO)","year":"2009","journal-title":"Dengue: Guidelines for Diagnosis, Treatment, Prevention and Control"},{"key":"pntd.0009205.ref002","unstructured":"World Health Organization (WHO). 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