{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T03:48:16Z","timestamp":1792900096411,"version":"4.5.0"},"update-to":[{"DOI":"10.1371\/journal.pntd.0009215","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2021,3,18]],"date-time":"2021-03-18T00:00:00Z","timestamp":1616025600000}}],"reference-count":37,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2021,3,8]],"date-time":"2021-03-08T00:00:00Z","timestamp":1615161600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"CONACyT","award":["FOSISS 2015-1 261482"],"award-info":[{"award-number":["FOSISS 2015-1 261482"]}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      The progressive disseminated histoplasmosis (PDH) has been associated with severe disease and high risk of death among people living with HIV (PLWHIV). Therefore, the purpose of this multicenter, prospective, double-blinded study done in ten Mexican hospitals was to determine the diagnostic accuracy of detecting\n                      <jats:italic>Histoplasma capsulatum<\/jats:italic>\n                      antigen in urine using the IMMY ALPHA\n                      <jats:italic>Histoplasma<\/jats:italic>\n                      EIA kit (IAHE), clarus\n                      <jats:italic>Histoplasma<\/jats:italic>\n                      GM Enzyme Immunoassay (cHGEI IMMY) and MiraVista\n                      <jats:italic>Histoplasma<\/jats:italic>\n                      Urine Antigen LFA (MVHUALFA); as well as the Hcp100 and 1281-1283\n                      <jats:sub>220<\/jats:sub>\n                      SCAR nested PCRs in blood, bone-marrow, tissue biopsies and urine.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methodology\/Principal findings<\/jats:title>\n                    <jats:p>\n                      We included 415 PLWHIV older than 18 years of age with suspicion of PDH. Using as diagnostic standard recovery of\n                      <jats:italic>H<\/jats:italic>\n                      .\n                      <jats:italic>capsulatum<\/jats:italic>\n                      in blood, bone marrow or tissue cultures, or histopathological exam compatible, detected 108 patients (26%, [95%CI, 21.78\u201330.22]) with proven-PDH. We analyzed 391 urine samples by the IAHE, cHGEI IMMY and MVHUALFA; the sensitivity\/specificity values obtained were 67.3% (95% CI, 57.4\u201376.2) \/ 96.2% (95% CI, 93.2\u201398.0) for IAHE, 91.3% (95% CI, 84.2\u201396.0) \/ 90.9% (95% CI, 87.0\u201394.0) for cHGEI IMMY and 90.4% (95% CI, 83.0\u201395.3) \/ 92.3% (95% CI, 88.6\u201395.1) for MVHUALFA.\n                    <\/jats:p>\n                    <jats:p>\n                      The Hcp100 nested PCR was performed on 393, 343, 75 and 297, blood, bone marrow, tissue and urine samples respectively; the sensitivity\/specificity values obtained were 62.9% (95%CI, 53.3\u201372.5)\/ 89.5% (95%CI, 86.0\u201393.0), 65.9% (95%CI, 56.0\u201375.8)\/ 89.0% (95%CI, 85.2\u201392.9), 62.1% (95%CI, 44.4\u201379.7)\/ 82.6% (95%CI, 71.7\u201393.6) and 34.9% (95%CI, 24.8\u201346.2)\/ 67.3% (95%CI, 60.6\u201373.5) respectively; and 1281-1283\n                      <jats:sub>220<\/jats:sub>\n                      SCAR nested PCR was performed on 392, 344, 75 and 291, respectively; the sensitivity\/specificity values obtained were 65.3% (95% CI, 55.9\u201374.7)\/ 58.8% (95%CI, 53.2\u201364.5), 70.8% (95%CI, 61.3\u201380.2)\/ 52.9% (95%CI, 46.8\u201359.1), 71.4% (95%CI, 54.7\u201388.2)\/ 40.4% (95%CI, 26.4\u201354.5) and 18.1% (95%CI, 10.5\u201328.1)\/ 90.4% (95%CI, 85.5\u201394.0), respectively.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Conclusions\/Significance<\/jats:title>\n                    <jats:p>The cHGEI IMMY and MVHUALFA tests showed excellent performance for the diagnosis of PDH in PLWHIV. The integration of these tests in clinical laboratories will certainly impact on early diagnosis and treatment.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pntd.0009215","type":"journal-article","created":{"date-parts":[[2021,3,8]],"date-time":"2021-03-08T13:40:08Z","timestamp":1615210808000},"page":"e0009215","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":60,"title":["Diagnostic accuracy of antigen detection in urine and molecular assays testing in different clinical samples for the diagnosis of progressive disseminated histoplasmosis in patients living with HIV\/AIDS: A prospective multicenter study in Mexico"],"prefix":"10.1371","volume":"15","author":[{"given":"Areli","family":"Mart\u00ednez-Gamboa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda 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