{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T05:19:38Z","timestamp":1790486378063,"version":"4.1.0"},"update-to":[{"DOI":"10.1371\/journal.pntd.0009231","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2021,3,24]],"date-time":"2021-03-24T00:00:00Z","timestamp":1616544000000}}],"reference-count":86,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2021,3,12]],"date-time":"2021-03-12T00:00:00Z","timestamp":1615507200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p><jats:italic>Salmonella<\/jats:italic> and <jats:italic>Shigella<\/jats:italic> bacteria are food- and waterborne pathogens that are responsible for enteric infections in humans and are still the major cause of morbidity and mortality in the emerging countries. The existence of multiple <jats:italic>Salmonella<\/jats:italic> and <jats:italic>Shigella<\/jats:italic> serotypes as well as the emergence of strains resistant to antibiotics requires the development of broadly protective therapies. Recently, the needle tip proteins of the type III secretion system of these bacteria were successfully utilized (SipD for <jats:italic>Salmonella<\/jats:italic> and IpaD for <jats:italic>Shigella<\/jats:italic>) as vaccine immunogens to provide good prophylactic cross-protection in murine models of infections. From these experiments, we have isolated a cross-protective monoclonal antibody directed against a conserved region of both proteins. Its conformational epitope determined by Deep Mutational Scanning is conserved among needle tip proteins of all pathogenic <jats:italic>Shigella<\/jats:italic> species and <jats:italic>Salmonella<\/jats:italic> serovars, and are well recognized by this antibody. Our study provides the first <jats:italic>in vivo<\/jats:italic> experimental evidence of the importance of this common region in the mechanism of virulence of <jats:italic>Salmonella<\/jats:italic> and <jats:italic>Shigella<\/jats:italic> and opens the way to the development of cross-protective therapeutic agents.<\/jats:p>","DOI":"10.1371\/journal.pntd.0009231","type":"journal-article","created":{"date-parts":[[2021,3,12]],"date-time":"2021-03-12T18:37:35Z","timestamp":1615574255000},"page":"e0009231","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":18,"title":["An antibody targeting type III secretion system induces broad protection against Salmonella and Shigella 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