{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T09:04:33Z","timestamp":1777539873280,"version":"3.51.4"},"update-to":[{"DOI":"10.1371\/journal.pntd.0009311","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2021,4,14]],"date-time":"2021-04-14T00:00:00Z","timestamp":1618358400000}}],"reference-count":94,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2021,4,2]],"date-time":"2021-04-02T00:00:00Z","timestamp":1617321600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"German Research Foundation","doi-asserted-by":"crossref","award":["TRR 228\/1"],"award-info":[{"award-number":["TRR 228\/1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001659","name":"German Research Foundation","doi-asserted-by":"crossref","award":["TRR 228\/1"],"award-info":[{"award-number":["TRR 228\/1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>Flaviviruses include a great diversity of mosquito-borne arboviruses with epidemic potential and high global disease burden. Several flaviviruses are circulating in southern Africa affecting humans and livestock, among them West Nile virus (WNV) and Wesselsbron virus. Despite their high relevance, no arbovirus surveillance study has been conducted for more than 35 years in Namibia. In this study we assessed the diversity of flaviviruses circulating in mosquitoes in the densely populated, semi-tropical Zambezi region of north-eastern Namibia. In total, 10,206 mosquitoes were sampled in Bwabwata and Mudumu national parks and Mashi and Wuparo conservancies and screened for flavivirus infections. A high infection rate with insect-specific flaviviruses was found with 241 strains of two previously known and seven putative novel insect-specific flaviviruses. In addition, we identified ten strains of WNV in the main vector <jats:italic>Cx<\/jats:italic>. <jats:italic>univittatus<\/jats:italic> sampled in the Mashi conservancy. Surprisingly, the strains fell into two different clades of lineage 2, 2b and 2d. Further, three strains of Bagaza Virus (BAGV) were found in <jats:italic>Cx<\/jats:italic>. <jats:italic>univittatus<\/jats:italic> mosquitoes originating from Mudumu national park. Assessment of BAGV growth in different cell lines showed high replication rates in mosquito and duck cells and about 100,000fold lower replication in human, primate and rodent cells. We demonstrate a wide genetic diversity of flaviviruses is circulating in mosquitoes in the Zambezi region. Importantly, WNV and BAGV can cause outbreaks including severe disease and mortality in humans and birds, respectively. Future studies should focus on WNV and BAGV geographic distribution, as well as on their potential health impacts in and the associated social and economic implications for southern Africa.<\/jats:p>","DOI":"10.1371\/journal.pntd.0009311","type":"journal-article","created":{"date-parts":[[2021,4,2]],"date-time":"2021-04-02T17:34:53Z","timestamp":1617384893000},"page":"e0009311","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":24,"title":["Simultaneous circulation of two West Nile virus lineage 2 clades and Bagaza virus in the Zambezi region, Namibia"],"prefix":"10.1371","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2310-1288","authenticated-orcid":true,"given":"Heiko D.","family":"Guggemos","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3347-6243","authenticated-orcid":true,"given":"Matthias","family":"Fendt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Hieke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Verena","family":"Heyde","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John K. 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Epidemics of West Nile and Sindbis viruses in South Africa with Culex (Culex) univittatus Theobald as vector."},{"issue":"6","key":"pntd.0009311.ref016","doi-asserted-by":"crossref","first-page":"877","DOI":"10.3201\/eid1506.081515","article-title":"Lineage 2 west nile virus as cause of fatal neurologic disease in horses, South Africa","volume":"15","author":"M Venter","year":"2009","journal-title":"Emerg Infect Dis"},{"issue":"7","key":"pntd.0009311.ref017","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1089\/vbz.2009.0230","article-title":"West Nile virus lineage 2 as a cause of zoonotic neurological disease in humans and horses in southern Africa","volume":"10","author":"M Venter","year":"2010","journal-title":"Vector Borne Zoonotic Dis"},{"issue":"4","key":"pntd.0009311.ref018","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1089\/vbz.2013.1407","article-title":"Use of envelope domain III protein for detection and differentiation of flaviviruses in the Free State Province, South Africa","volume":"14","author":"L Mathengtheng","year":"2014","journal-title":"Vector Borne Zoonotic Dis."},{"issue":"12","key":"pntd.0009311.ref019","doi-asserted-by":"crossref","first-page":"2290","DOI":"10.3201\/eid2512.190572","article-title":"West Nile Virus in Wildlife and Nonequine Domestic Animals, South Africa, 2010\u20132018","volume":"25","author":"J Steyn","year":"2019","journal-title":"Emerg Infect Dis"},{"issue":"5","key":"pntd.0009311.ref020","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1089\/vbz.2012.1181","article-title":"Human cases of Wesselsbron disease, South Africa 2010\u20132011","volume":"13","author":"J Weyer","year":"2013","journal-title":"Vector Borne Zoonotic Dis"},{"issue":"5","key":"pntd.0009311.ref021","doi-asserted-by":"crossref","DOI":"10.3390\/pathogens9050373","article-title":"Early Pathogenesis of Wesselsbron Disease in Pregnant Ewes","volume":"9","author":"J Oymans","year":"2020","journal-title":"Pathogens."},{"key":"pntd.0009311.ref022","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.onehlt.2017.02.001","article-title":"Emergence of Wesselsbron virus among black rat and humans in Eastern Senegal in 2013","volume":"3","author":"MM Diagne","year":"2017","journal-title":"One Health."},{"issue":"1","key":"pntd.0009311.ref023","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1017\/S0022172400027066","article-title":"An investigation of flavivirus infections of cattle in Zimbabwe Rhodesia with particular reference to Wesselsbron virus","volume":"85","author":"NK Blackburn","year":"1980","journal-title":"J Hyg (Lond)."},{"key":"pntd.0009311.ref024","author":"JA Coetzer","year":"1977","journal-title":"Hydrops amnii in sheep associated with hydranencephaly and arthrogryposis with wesselsbron disease and rift valley fever viruses as aetiological 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Digoutte","year":"1978","journal-title":"Bagaza (BAG)."},{"issue":"6","key":"pntd.0009311.ref033","first-page":"742","volume":"47","author":"SW Gordon","year":"1992","journal-title":"Arbovirus Isolations from Mosquitoes Collected During 1988 in the Senegal River Basin"},{"issue":"6","key":"pntd.0009311.ref034","doi-asserted-by":"crossref","first-page":"934","DOI":"10.1093\/jmedent\/31.6.934","article-title":"Isolations of West Nile and Bagaza viruses from mosquitoes (Diptera: Culicidae) in central Senegal (Ferlo)","volume":"31","author":"M Traore-Lamizana","year":"1994","journal-title":"J Med Entomol"},{"issue":"2","key":"pntd.0009311.ref035","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1111\/j.0269-283X.2005.00564.x","article-title":"Mosquito vectors of the 1998\u20131999 outbreak of Rift Valley Fever and other arboviruses (Bagaza, Sanar, Wesselsbron and West Nile) in Mauritania and Senegal","volume":"19","author":"M Diallo","year":"2005","journal-title":"Med Vet 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arboviruses of African origin: review of key viruses and vectors","volume":"11","author":"L Braack","year":"2018","journal-title":"Parasit Vectors."},{"key":"pntd.0009311.ref040","first-page":"69","article-title":"Mosquito-borne virus diseases of man in southern Africa","volume":"Suppl","author":"BM McIntosh","year":"1986","journal-title":"S Afr Med J"},{"issue":"Suppl 1","key":"pntd.0009311.ref041","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1179\/2046904712Z.00000000048","article-title":"The dengue situation in Africa","volume":"32","author":"F. Were","year":"2012","journal-title":"Paediatr Int Child Health"},{"issue":"5","key":"pntd.0009311.ref042","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1016\/0035-9203(65)90158-6","article-title":"Survey for antibodies against arthropod-borne viruses in the sera of indigenous residents of the Caprivi Strip and Bechuanaland Protectorate","volume":"59","author":"RH Kokernot","year":"1965","journal-title":"Trans R Soc Trop Med Hyg."},{"issue":"1","key":"pntd.0009311.ref043","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/0035-9203(91)90176-Y","article-title":"Serological markers of hepatitis B virus and certain other viruses in the population of eastern Caprivi, Namibia","volume":"85","author":"JJ Joubert","year":"1991","journal-title":"Trans R Soc Trop Med Hyg"},{"issue":"8","key":"pntd.0009311.ref044","doi-asserted-by":"crossref","first-page":"820","DOI":"10.3201\/eid0808.020027","article-title":"Phylogenetic relationships of southern African West Nile virus isolates","volume":"8","author":"FJ Burt","year":"2002","journal-title":"Emerg Infect Dis."},{"key":"pntd.0009311.ref045","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.actatropica.2014.05.010","article-title":"Risk assessment of flavivirus transmission in Namibia","volume":"137","author":"BH Noden","year":"2014","journal-title":"Acta Trop."},{"key":"pntd.0009311.ref046","author":"Pupae. Edwards FWJMotERI-CA","year":"1941","journal-title":"Mosquitoes of the Ethiopian Region. III.-Culicine adults and pupae"},{"key":"pntd.0009311.ref047","author":"MT Gillies","year":"1968","journal-title":"The Anophelinae of Africa south of the Sahara (Ethiopian zoogeographical region)"},{"key":"pntd.0009311.ref048","volume-title":"Mosquitoes of Southern Africa: culicinae and toxorhynchitinae","author":"PG Jupp","year":"1996"},{"issue":"Pt 7","key":"pntd.0009311.ref049","doi-asserted-by":"crossref","first-page":"1971","DOI":"10.1099\/vir.0.79850-0","article-title":"Sequences of flavivirus-related RNA viruses persist in DNA form integrated in the genome of Aedes spp. mosquitoes","volume":"85","author":"S Crochu","year":"2004","journal-title":"J Gen Virol"},{"issue":"4","key":"pntd.0009311.ref050","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1089\/vbz.2007.0206","article-title":"A real-time RT-PCR method for the universal detection and identification of flaviviruses","volume":"7","author":"G Moureau","year":"2007","journal-title":"Vector Borne Zoonotic Dis."},{"issue":"5","key":"pntd.0009311.ref051","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1177\/104063871002200515","article-title":"Two new real-time quantitative reverse transcription polymerase chain reaction assays with unique target sites for the specific and sensitive detection of lineages 1 and 2 West Nile virus strains","volume":"22","author":"M Eiden","year":"2010","journal-title":"J Vet Diagn Invest."},{"issue":"7","key":"pntd.0009311.ref052","doi-asserted-by":"crossref","first-page":"2323","DOI":"10.1128\/JCM.40.7.2323-2330.2002","article-title":"Rapid detection and quantification of RNA of Ebola and Marburg viruses, Lassa virus, Crimean-Congo hemorrhagic fever virus, Rift Valley fever virus, dengue virus, and yellow fever virus by real-time reverse transcription-PCR","volume":"40","author":"C Drosten","year":"2002","journal-title":"J Clin Microbiol"},{"issue":"12","key":"pntd.0009311.ref053","doi-asserted-by":"crossref","first-page":"880","DOI":"10.2471\/BLT.16.175950","article-title":"Assay optimization for molecular detection of Zika virus","volume":"94","author":"VM Corman","year":"2016","journal-title":"Bull World Health Organ"},{"issue":"12","key":"pntd.0009311.ref054","doi-asserted-by":"crossref","first-page":"4054","DOI":"10.1128\/JCM.01799-12","article-title":"Advanced yellow fever virus genome detection in point-of-care facilities and reference laboratories","volume":"50","author":"C Domingo","year":"2012","journal-title":"J Clin Microbiol"},{"issue":"22","key":"pntd.0009311.ref055","doi-asserted-by":"crossref","first-page":"4673","DOI":"10.1093\/nar\/22.22.4673","article-title":"CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice","volume":"22","author":"JD Thompson","year":"1994","journal-title":"Nucleic Acids Res"},{"issue":"14","key":"pntd.0009311.ref056","doi-asserted-by":"crossref","first-page":"3059","DOI":"10.1093\/nar\/gkf436","article-title":"MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform","volume":"30","author":"K Katoh","year":"2002","journal-title":"Nucleic Acids Res"},{"issue":"6","key":"pntd.0009311.ref057","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms","volume":"35","author":"S Kumar","year":"2018","journal-title":"Mol Biol Evol"},{"issue":"3","key":"pntd.0009311.ref058","first-page":"307","volume":"59","author":"S Guindon","year":"2010","journal-title":"New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0"},{"issue":"5","key":"pntd.0009311.ref059","first-page":"294","article-title":"DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates","volume":"3","author":"O Folmer","year":"1994","journal-title":"Mol Mar Biol Biotechnol"},{"issue":"9","key":"pntd.0009311.ref060","doi-asserted-by":"crossref","first-page":"e7092","DOI":"10.1371\/journal.pone.0007092","article-title":"Disentangling vector-borne transmission networks: a universal DNA barcoding method to identify vertebrate hosts from arthropod bloodmeals","volume":"4","author":"M Alcaide","year":"2009","journal-title":"PLoS One"},{"issue":"9","key":"pntd.0009311.ref061","doi-asserted-by":"crossref","first-page":"4462","DOI":"10.1128\/JVI.00014-09","article-title":"A new flavivirus and a new vector: characterization of a novel flavivirus isolated from uranotaenia mosquitoes from a tropical rain forest","volume":"83","author":"S Junglen","year":"2009","journal-title":"J Virol"},{"issue":"3","key":"pntd.0009311.ref062","first-page":"493","volume":"27","author":"LJ Reed","year":"1938","journal-title":"A simple method of estimating fifty per cent endpoints"},{"issue":"23","key":"pntd.0009311.ref063","doi-asserted-by":"crossref","first-page":"12850","DOI":"10.1128\/JVI.01862-13","article-title":"Discovery of a unique novel clade of mosquito-associated bunyaviruses","volume":"87","author":"M Marklewitz","year":"2013","journal-title":"J Virol"},{"issue":"2","key":"pntd.0009311.ref064","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1006\/viro.1998.9290","article-title":"The DF-1 chicken fibroblast cell line: transformation induced by diverse oncogenes and cell death resulting from infection by avian leukosis viruses","volume":"248","author":"M Himly","year":"1998","journal-title":"Virology"},{"issue":"16","key":"pntd.0009311.ref065","first-page":"4460","article-title":"Establishment and characterization of a chicken hepatocellular carcinoma cell line","volume":"47","author":"T Kawaguchi","year":"1987","journal-title":"LMH. Cancer Res"},{"issue":"5","key":"pntd.0009311.ref066","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1016\/j.vaccine.2008.11.066","article-title":"An avian cell line designed for production of highly attenuated viruses","volume":"27","author":"I Jordan","year":"2009","journal-title":"Vaccine"},{"issue":"Pt 2","key":"pntd.0009311.ref067","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1099\/vir.0.046888-0","article-title":"Molecular evolution of lineage 2 West Nile virus","volume":"94","author":"AR McMullen","year":"2013","journal-title":"J Gen Virol"},{"issue":"1665","key":"pntd.0009311.ref068","article-title":"Climate change impacts on West Nile virus transmission in a global context","volume":"370","author":"S. Paz","year":"2015","journal-title":"Philos Trans R Soc Lond B Biol Sci"},{"key":"pntd.0009311.ref069","unstructured":"World Weather Online [cited 2020 09\/12]. 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