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To understand the level of past DENV infection among older adults after a large dengue outbreak occurred in southern Taiwan in 2015, we screened 1498 and 2603 serum samples from healthy residents aged \u2265 40 years in Kaohsiung City and Tainan City, respectively, to assess the seroprevalence of anti-DENV IgG in 2016. Seropositive samples were verified to exclude cross-reaction from Japanese encephalitis virus (JEV), using DENV\/JEV-NS1 indirect IgG ELISA. We further identified viral serotypes and secondary DENV infections among positive samples in the two cities. The overall age-standardized seroprevalence of DENV-IgG among participants was 25.77% in Kaohsiung and 11.40% in Tainan, and the seroprevalence was significantly higher in older age groups of both cities. Although the percentages of secondary DENV infection in Kaohsiung and Tainan were very similar (43.09% and 44.76%, respectively), DENV-1 and DENV-2 spanned a wider age range in Kaohsiung, whereas DENV-2 was dominant in Tainan. As very few studies have obtained the serostatus of DENV infection in older adults and the elderly, this study highlights the need for further investigation into antibody status, as well as the safety and efficacy of dengue vaccination in these older populations.<\/jats:p>","DOI":"10.1371\/journal.pntd.0009312","type":"journal-article","created":{"date-parts":[[2021,4,1]],"date-time":"2021-04-01T14:40:08Z","timestamp":1617288008000},"page":"e0009312","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":7,"title":["Use of seroprevalence to guide dengue vaccination plans for older adults in a dengue non-endemic country"],"prefix":"10.1371","volume":"15","author":[{"given":"Yi-Hua","family":"Pan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mei-Ying","family":"Liao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5662-8751","authenticated-orcid":true,"given":"Yu-Wen","family":"Chien","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2951-6679","authenticated-orcid":true,"given":"Tzong-Shiann","family":"Ho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9618-4710","authenticated-orcid":true,"given":"Hui-Ying","family":"Ko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0178-7744","authenticated-orcid":true,"given":"Chin-Rur","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shu-Fen","family":"Chang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chia-Yi","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shu-Yu","family":"Lin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pin-Wei","family":"Shih","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pei-Yun","family":"Shu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Day-Yu","family":"Chao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao-Ying","family":"Pan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong-Ming","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guey-Chuen","family":"Perng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chia-Chi","family":"Ku","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6078-2601","authenticated-orcid":true,"given":"Chwan-Chuen","family":"King","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2021,4,1]]},"reference":[{"issue":"6","key":"pntd.0009312.ref001","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1016\/S1473-3099(16)00026-8","article-title":"The global burden of dengue: an analysis from the Global Burden of Disease Study 2013","volume":"16","author":"JD Stanaway","year":"2016","journal-title":"Lancet Infect Dis"},{"key":"pntd.0009312.ref002","doi-asserted-by":"crossref","first-page":"e08347","DOI":"10.7554\/eLife.08347","article-title":"The global distribution of the arbovirus vectors Aedes aegypti and Ae. albopictus","volume":"4","author":"MU Kraemer","year":"2015","journal-title":"Elife"},{"key":"pntd.0009312.ref003","article-title":"Spatial analysis of dengue incidence and Aedes aegypti ovitrap surveillance in Belo Horizonte, Brazil","author":"OL Schultes","year":"2020","journal-title":"Trop Med Int Health"},{"key":"pntd.0009312.ref004","unstructured":"WHO. 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The 1980 outbreak","volume":"120","author":"N Sangkawibha","year":"1984","journal-title":"Am J Epidemiol"},{"issue":"5","key":"pntd.0009312.ref068","doi-asserted-by":"crossref","first-page":"566","DOI":"10.4269\/ajtmh.1997.56.566","article-title":"Risk factors in dengue shock syndrome","volume":"56","author":"S Thein","year":"1997","journal-title":"Am J Trop Med Hyg"},{"issue":"4","key":"pntd.0009312.ref069","doi-asserted-by":"crossref","first-page":"635","DOI":"10.4269\/ajtmh.1980.29.635","article-title":"Transmission of dengue 1 and 2 viruses in Greece in 1928","volume":"29","author":"SB Halstead","year":"1980","journal-title":"Am J Trop Med Hyg"},{"issue":"2","key":"pntd.0009312.ref070","doi-asserted-by":"crossref","first-page":"179","DOI":"10.4269\/ajtmh.1990.42.179","article-title":"Dengue hemorrhagic fever in Cuba, 1981: a retrospective seroepidemiologic study","volume":"42","author":"MG Guzm\u00e1n","year":"1990","journal-title":"Am J Trop Med Hyg"},{"issue":"6","key":"pntd.0009312.ref071","doi-asserted-by":"crossref","first-page":"1026","DOI":"10.1093\/infdis\/jit273","article-title":"Reduced risk of disease during postsecondary dengue virus infections","volume":"208","author":"S Olkowski","year":"2013","journal-title":"J Infect Dis"},{"issue":"11","key":"pntd.0009312.ref072","article-title":"Seasonal Patterns of Japanese Encephalitis and Associated Meteorological Factors in Taiwan","volume":"14","author":"CL Lin","year":"2017","journal-title":"Int J Environ Res Public Health"},{"key":"pntd.0009312.ref073","unstructured":"Huang J-J. Disease control of Japanese Encephalitis: 2018 Kaohsiung strategies (In Chinese)2018."},{"issue":"1","key":"pntd.0009312.ref074","doi-asserted-by":"crossref","first-page":"12","DOI":"10.4269\/ajtmh.1996.55.12","article-title":"Silent transmission of the dengue virus in southern Taiwan","volume":"55","author":"WJ Chen","year":"1996","journal-title":"Am J Trop Med Hyg"},{"issue":"3","key":"pntd.0009312.ref075","doi-asserted-by":"crossref","first-page":"552","DOI":"10.3201\/eid1003.020518","article-title":"1998 dengue hemorrhagic fever epidemic in Taiwan","volume":"10","author":"DY Chao","year":"2004","journal-title":"Emerg Infect Dis"},{"key":"pntd.0009312.ref076","unstructured":"Taiwan National Infectious Disease Statistics System-ZIKA virus [Internet]. 2020. https:\/\/nidss.cdc.gov.tw\/nndss\/disease?id=ZIKAV."},{"issue":"1","key":"pntd.0009312.ref077","doi-asserted-by":"crossref","first-page":"884","DOI":"10.1186\/s12879-019-4491-4","article-title":"Low seroprevalence of Zika virus 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Infectious Diseases"},{"issue":"11","key":"pntd.0009312.ref081","doi-asserted-by":"crossref","first-page":"e3159","DOI":"10.1371\/journal.pntd.0003159","article-title":"Epidemiology of dengue disease in Malaysia (2000\u20132012): a systematic literature review","volume":"8","author":"AH Mohd-Zaki","year":"2014","journal-title":"PLoS Negl Trop Dis"},{"issue":"1","key":"pntd.0009312.ref082","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1017\/S0950268898001617","article-title":"Changing epidemiology of dengue hemorrhagic fever in Thailand","volume":"122","author":"O Chareonsook","year":"1999","journal-title":"Epidemiol Infect"},{"issue":"10","key":"pntd.0009312.ref083","doi-asserted-by":"crossref","first-page":"1297","DOI":"10.1093\/cid\/ciw511","article-title":"Effect of a Dengue Clinical Case Management Course on Physician Practices in Puerto Rico","volume":"63","author":"GS Han","year":"2016","journal-title":"Clin Infect 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