{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T09:03:35Z","timestamp":1784192615858,"version":"3.55.0"},"reference-count":54,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2021,4,16]],"date-time":"2021-04-16T00:00:00Z","timestamp":1618531200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81802031"],"award-info":[{"award-number":["81802031"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31771286"],"award-info":[{"award-number":["31771286"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2018A030310074"],"award-info":[{"award-number":["2018A030310074"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Social science and technology research project of Zhongshan","award":["2018B1001"],"award-info":[{"award-number":["2018B1001"]}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p><jats:italic>Trichomonas vaginalis<\/jats:italic> is a common protozoan parasite, which causes trichomoniasis associated with severe adverse reproductive outcomes. However, the underlying pathogenesis has not been fully understood. As the first line of defense against invading pathogens, the vaginal epithelial cells are highly responsive to environmental stimuli and contribute to the formation of the optimal luminal fluid microenvironment. The cystic fibrosis transmembrane conductance regulator (CFTR), an anion channel widely distributed at the apical membrane of epithelial cells, plays a crucial role in mediating the secretion of Cl<jats:sup>\u2212<\/jats:sup> and HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>. In this study, we investigated the effect of <jats:italic>T<\/jats:italic>. <jats:italic>vaginalis<\/jats:italic> on vaginal epithelial ion transport elicited by prostaglandin E<jats:sub>2<\/jats:sub> (PGE<jats:sub>2<\/jats:sub>), a major prostaglandin in the semen. Luminal administration of PGE<jats:sub>2<\/jats:sub> triggered a remarkable and sustained increase of short-circuit current (<jats:italic>I<\/jats:italic><jats:sub>SC<\/jats:sub>) in rat vaginal epithelium, which was mainly due to Cl<jats:sup>\u2212<\/jats:sup> and HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> secretion mediated by the cAMP-activated CFTR. However, <jats:italic>T<\/jats:italic>. <jats:italic>vaginalis<\/jats:italic> infection significantly abrogated the <jats:italic>I<\/jats:italic><jats:sub>SC<\/jats:sub> response evoked by PGE<jats:sub>2<\/jats:sub>, indicating impaired transepithelial anion transport via CFTR. Using a primary cell culture system of rat vaginal epithelium and a human vaginal epithelial cell line, we demonstrated that the expression of CFTR was significantly down-regulated after <jats:italic>T<\/jats:italic>. <jats:italic>vaginalis<\/jats:italic> infection. In addition, defective Cl<jats:sup>\u2212<\/jats:sup> transport function of CFTR was observed in <jats:italic>T<\/jats:italic>. <jats:italic>vaginalis<\/jats:italic>-infected cells by measuring intracellular Cl<jats:sup>\u2212<\/jats:sup> signals. Conclusively, <jats:italic>T<\/jats:italic>. <jats:italic>vaginalis<\/jats:italic> restrained exogenous PGE<jats:sub>2<\/jats:sub>-induced anion secretion through down-regulation of CFTR in vaginal epithelium. These results provide novel insights into the intervention of reproductive complications associated with <jats:italic>T<\/jats:italic>. <jats:italic>vaginalis<\/jats:italic> infection such as infertility and disequilibrium in vaginal fluid microenvironment.<\/jats:p>","DOI":"10.1371\/journal.pntd.0009319","type":"journal-article","created":{"date-parts":[[2021,4,16]],"date-time":"2021-04-16T17:28:32Z","timestamp":1618594112000},"page":"e0009319","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":7,"title":["Trichomonas vaginalis infection impairs anion secretion in vaginal 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