{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T18:43:20Z","timestamp":1787769800506,"version":"build-2784847793"},"update-to":[{"DOI":"10.1371\/journal.pntd.0009336","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2021,4,29]],"date-time":"2021-04-29T00:00:00Z","timestamp":1619654400000}}],"reference-count":68,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2021,4,19]],"date-time":"2021-04-19T00:00:00Z","timestamp":1618790400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100011102","name":"Seventh Framework Programme","doi-asserted-by":"publisher","award":["281803"],"award-info":[{"award-number":["281803"]}],"id":[{"id":"10.13039\/100011102","id-type":"DOI","asserted-by":"publisher"}]},{"name":"European Union\u2019s Horizon 2020 research and innovation program","award":["734548"],"award-info":[{"award-number":["734548"]}]},{"DOI":"10.13039\/501100004586","name":"Faperj","doi-asserted-by":"crossref","award":["E-26\/202.930\/2016"],"award-info":[{"award-number":["E-26\/202.930\/2016"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100006506","name":"Brazilian Ministry of Health","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100006506","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Background<\/jats:title>\n<jats:p>Serological diagnosis of Zika virus (ZIKV) infection is challenging because of the antibody cross-reactivity among flaviviruses. At the same time, the role of Nucleic Acid Testing (NAT) is limited by the low proportion of symptomatic infections and the low average viral load. Here, we compared the diagnostic performance of commercially available IgM, IgAM, and IgG ELISAs in sequential samples during the ZIKV and chikungunya (CHIKV) epidemics and co-circulation of dengue virus (DENV) in Brazil and Venezuela.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methodology\/Principal findings<\/jats:title>\n<jats:p>Acute (day of illness 1\u20135) and follow-up (day of illness \u2265 6) blood samples were collected from nine hundred and seven symptomatic patients enrolled in a prospective multicenter study between June 2012 and August 2016. Acute samples were tested by RT-PCR for ZIKV, DENV, and CHIKV. Acute and follow-up samples were tested for IgM, IgAM, and IgG antibodies to ZIKV using commercially available ELISAs. Among follow-up samples with a RT-PCR confirmed ZIKV infection, anti-ZIKV IgAM sensitivity was 93.5% (43\/46), while IgM and IgG exhibited sensitivities of 30.3% (10\/33) and 72% (18\/25), respectively. An additional 24% (26\/109) of ZIKV infections were detected via IgAM seroconversion in ZIKV\/DENV\/CHIKV RT-PCR negative patients. The specificity of anti-ZIKV IgM was estimated at 93% and that of IgAM at 85%.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Conclusions\/Significance<\/jats:title>\n<jats:p>Our findings exemplify the challenges of the assessment of test performance for ZIKV serological tests in the real-world setting, during co-circulation of DENV, ZIKV, and CHIKV. However, we can also demonstrate that the IgAM immunoassay exhibits superior sensitivity to detect ZIKV RT-PCR confirmed infections compared to IgG and IgM immunoassays. The IgAM assay also proves to be promising for detection of anti-ZIKV seroconversions in sequential samples, both in ZIKV PCR-positive as well as PCR-negative patients, making this a candidate assay for serological monitoring of pregnant women in future ZIKV outbreaks.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pntd.0009336","type":"journal-article","created":{"date-parts":[[2021,4,19]],"date-time":"2021-04-19T20:58:13Z","timestamp":1618865893000},"page":"e0009336","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":17,"title":["Diagnostic performance of anti-Zika virus IgM, IgAM and IgG ELISAs during co-circulation of Zika, dengue, and chikungunya viruses in Brazil and Venezuela"],"prefix":"10.1371","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6803-9284","authenticated-orcid":true,"given":"Ivonne","family":"Morales","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3459-3812","authenticated-orcid":true,"given":"Kerstin D.","family":"Rosenberger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tereza","family":"Magalhaes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0887-8163","authenticated-orcid":true,"given":"Clarice N. L.","family":"Morais","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7862-6455","authenticated-orcid":true,"given":"Cynthia","family":"Braga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3826-9358","authenticated-orcid":true,"given":"Ernesto T. A.","family":"Marques","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3545-5238","authenticated-orcid":true,"given":"Guilherme Amaral","family":"Calvet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1724-598X","authenticated-orcid":true,"given":"Luana","family":"Damasceno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Brasil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9815-9022","authenticated-orcid":true,"given":"Ana Maria","family":"Bispo de Filippis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1918-9144","authenticated-orcid":true,"given":"Adriana","family":"Tami","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Bethencourt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayling","family":"Alvarez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9886-6448","authenticated-orcid":true,"given":"Pedro A.","family":"Mart\u00ednez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria G.","family":"Guzman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1697-3427","authenticated-orcid":true,"given":"Bruno","family":"Souza Benevides","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1972-8205","authenticated-orcid":true,"given":"Andrea","family":"Caprara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5417-2738","authenticated-orcid":true,"given":"Nguyen Than Ha","family":"Quyen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9039-7392","authenticated-orcid":true,"given":"Cameron P.","family":"Simmons","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9086-8804","authenticated-orcid":true,"given":"Bridget","family":"Wills","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"de Lamballerie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan Felix","family":"Drexler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8252-7444","authenticated-orcid":true,"given":"Thomas","family":"Jaenisch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"the IDAMS Clinical Study Group","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,4,19]]},"reference":[{"issue":"5","key":"pntd.0009336.ref001","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1016\/0035-9203(52)90042-4","article-title":"Zika virus. I. Isolations and serological specificity","volume":"46","author":"GW Dick","year":"1952","journal-title":"Trans R Soc Trop Med Hyg"},{"issue":"1","key":"pntd.0009336.ref002","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1038\/s41426-018-0082-5","article-title":"Re-visiting the evolution, dispersal and epidemiology of Zika virus in Asia","volume":"7","author":"JH Pettersson","year":"2018","journal-title":"Emerg Microbes Infect"},{"issue":"24","key":"pntd.0009336.ref003","doi-asserted-by":"crossref","first-page":"2536","DOI":"10.1056\/NEJMoa0805715","article-title":"Zika virus outbreak on Yap Island, Federated States of Micronesia","volume":"360","author":"MR Duffy","year":"2009","journal-title":"N Engl J Med"},{"issue":"6","key":"pntd.0009336.ref004","first-page":"1085","article-title":"Zika virus, French polynesia, South pacific, 2013","volume":"20","author":"VM Cao-Lormeau","year":"2014","journal-title":"Emerg Infect Dis"},{"issue":"2","key":"pntd.0009336.ref005","doi-asserted-by":"crossref","first-page":"381","DOI":"10.3201\/eid2102.141553","article-title":"Co-infection with Zika and dengue viruses in 2 patients, New Caledonia, 2014","volume":"21","author":"M Dupont-Rouzeyrol","year":"2015","journal-title":"Emerg Infect Dis"},{"issue":"3","key":"pntd.0009336.ref006","article-title":"Boletim epidemiol\u00f3gico: Monitoramento dos casos de dengue, febre de chikungunya e febre pelo v\u00edrus Zika at\u00e9 a Semana Epidemiol\u00f3gica 52, 2016","volume":"48","author":"SdVe Sa\u00fade","year":"2017","journal-title":"Minist\u00e9rio da Sa\u00fade do Brasil, 2017"},{"issue":"24","key":"pntd.0009336.ref007","doi-asserted-by":"crossref","first-page":"2321","DOI":"10.1056\/NEJMoa1602412","article-title":"Zika Virus Infection in Pregnant Women in Rio de Janeiro","volume":"375","author":"P Brasil","year":"2016","journal-title":"N Engl J Med"},{"issue":"4","key":"pntd.0009336.ref008","doi-asserted-by":"crossref","first-page":"598","DOI":"10.2105\/AJPH.2016.303115","article-title":"Initial Description of the Presumed Congenital Zika Syndrome","volume":"106","author":"B Miranda-Filho Dde","year":"2016","journal-title":"Am J Public Health"},{"issue":"10","key":"pntd.0009336.ref009","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1056\/NEJMoa1600651","article-title":"Zika Virus Associated with Microcephaly","volume":"374","author":"J Mlakar","year":"2016","journal-title":"N Engl J Med"},{"issue":"5","key":"pntd.0009336.ref010","doi-asserted-by":"crossref","first-page":"e172","DOI":"10.1016\/S1473-3099(17)30446-2","article-title":"Zika virus in French Polynesia 2013\u201314: anatomy of a completed outbreak","volume":"18","author":"D Musso","year":"2018","journal-title":"Lancet Infect Dis"},{"key":"pntd.0009336.ref011","unstructured":"Organization WH. WHO statement on the first meeting of the International Health Regulations (2005) (IHR 2005) Emergency Committee on Zika virus and observed increase in neurological disorders and neonatal malformations. 2016."},{"issue":"13","key":"pntd.0009336.ref012","doi-asserted-by":"crossref","DOI":"10.2807\/1560-7917.ES2014.19.13.20751","article-title":"Evidence of perinatal transmission of Zika virus, French Polynesia, December 2013 and February 2014","volume":"19","author":"M Besnard","year":"2014","journal-title":"Euro Surveill"},{"issue":"5","key":"pntd.0009336.ref013","doi-asserted-by":"crossref","first-page":"880","DOI":"10.3201\/eid1705.101939","article-title":"Probable non-vector-borne transmission of Zika virus, Colorado, USA","volume":"17","author":"BD Foy","year":"2011","journal-title":"Emerg Infect Dis"},{"issue":"6300","key":"pntd.0009336.ref014","doi-asserted-by":"crossref","first-page":"aaf8160","DOI":"10.1126\/science.aaf8160","article-title":"Assessing the global threat from Zika virus","volume":"353","author":"J Lessler","year":"2016","journal-title":"Science"},{"issue":"4","key":"pntd.0009336.ref015","doi-asserted-by":"crossref","first-page":"e0004636","DOI":"10.1371\/journal.pntd.0004636","article-title":"Zika Virus Outbreak in Rio de Janeiro, Brazil: Clinical Characterization, Epidemiological and Virological Aspects","volume":"10","author":"P Brasil","year":"2016","journal-title":"PLoS Negl Trop Dis"},{"issue":"6","key":"pntd.0009336.ref016","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1017\/S0950268818000419","article-title":"Zika virus epidemiology: from Uganda to world pandemic, an update","volume":"146","author":"C Talero-Gutierrez","year":"2018","journal-title":"Epidemiol Infect"},{"issue":"3","key":"pntd.0009336.ref017","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1016\/S1473-3099(19)30547-X","article-title":"Age-dependent manifestations and case definitions of paediatric Zika: a prospective cohort study","volume":"20","author":"R Burger-Calderon","year":"2020","journal-title":"Lancet Infect Dis"},{"issue":"2","key":"pntd.0009336.ref018","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1093\/cid\/ciz195","article-title":"Postnatally Acquired Zika Virus Disease Among Children, United States, 2016\u20132017","volume":"70","author":"NP Lindsey","year":"2020","journal-title":"Clin Infect Dis"},{"issue":"6","key":"pntd.0009336.ref019","doi-asserted-by":"crossref","first-page":"402","DOI":"10.2471\/BLT.17.201541","article-title":"Prevalence of asymptomatic Zika virus infection: a systematic review","volume":"96","author":"MM Haby","year":"2018","journal-title":"Bull World Health Organ"},{"key":"pntd.0009336.ref020","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.jviromet.2018.10.011","article-title":"Validation of serological and molecular methods for diagnosis of zika virus infections","volume":"263","author":"AJ Jaaskelainen","year":"2019","journal-title":"J Virol Methods"},{"key":"pntd.0009336.ref021","unstructured":"PAHO. Zika Resources: Case Definitions 2016 [updated April 1, 2016. Available from: https:\/\/www.paho.org\/hq\/index.php?option=com_content&view=article&id=11117:zika-resources-case-definitions&Itemid=41532&lang=en."},{"issue":"1","key":"pntd.0009336.ref022","doi-asserted-by":"crossref","first-page":"4012","DOI":"10.1038\/s41598-018-22159-2","article-title":"Efficient detection of Zika virus RNA in patients\u2019 blood from the 2016 outbreak in Campinas, Brazil","volume":"8","author":"CC Judice","year":"2018","journal-title":"Sci Rep"},{"issue":"1","key":"pntd.0009336.ref023","doi-asserted-by":"crossref","first-page":"60","DOI":"10.5858\/arpa.2016-0406-SA","article-title":"Laboratory Diagnosis of Zika Virus Infection","volume":"141","author":"ML Landry","year":"2017","journal-title":"Arch Pathol Lab Med"},{"issue":"suppl_10","key":"pntd.0009336.ref024","doi-asserted-by":"crossref","first-page":"S951","DOI":"10.1093\/infdis\/jix502","article-title":"Diagnosis of Zika Virus Infections: Challenges and Opportunities","volume":"216","author":"JL Munoz-Jordan","year":"2017","journal-title":"J Infect Dis"},{"issue":"12","key":"pntd.0009336.ref025","doi-asserted-by":"crossref","first-page":"880","DOI":"10.2471\/BLT.16.175950","article-title":"Assay optimization for molecular detection of Zika virus","volume":"94","author":"VM Corman","year":"2016","journal-title":"Bull World Health Organ"},{"issue":"18","key":"pntd.0009336.ref026","doi-asserted-by":"crossref","first-page":"475","DOI":"10.15585\/mmwr.mm6518e2","article-title":"Comparison of Test Results for Zika Virus RNA in Urine, Serum, and Saliva Specimens from Persons with Travel-Associated Zika Virus Disease\u2014Florida, 2016","volume":"65","author":"AM Bingham","year":"2016","journal-title":"MMWR Morb Mortal Wkly Rep"},{"issue":"1","key":"pntd.0009336.ref027","doi-asserted-by":"crossref","first-page":"84","DOI":"10.3201\/eid2101.140894","article-title":"Detection of Zika virus in urine","volume":"21","author":"AC Gourinat","year":"2015","journal-title":"Emerg Infect Dis"},{"issue":"26","key":"pntd.0009336.ref028","doi-asserted-by":"crossref","DOI":"10.2807\/1560-7917.ES.2016.21.26.30269","article-title":"Detection of Zika virus RNA in whole blood of imported Zika virus disease cases up to 2 months after symptom onset, Israel, December 2015 to April 2016","volume":"21","author":"Y Lustig","year":"2016","journal-title":"Euro Surveill"},{"issue":"5","key":"pntd.0009336.ref029","doi-asserted-by":"crossref","first-page":"863","DOI":"10.3201\/eid2305.161631","article-title":"Zika Virus Infection and Prolonged Viremia in Whole-Blood Specimens","volume":"23","author":"JM Mansuy","year":"2017","journal-title":"Emerg Infect Dis"},{"key":"pntd.0009336.ref030","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.jcv.2015.04.021","article-title":"Detection of Zika virus in saliva","volume":"68","author":"D Musso","year":"2015","journal-title":"J Clin Virol"},{"issue":"13","key":"pntd.0009336.ref031","doi-asserted-by":"crossref","first-page":"1234","DOI":"10.1056\/NEJMoa1613108","article-title":"Persistence of Zika Virus in Body Fluids\u2014Final Report","volume":"379","author":"G Paz-Bailey","year":"2018","journal-title":"N Engl J Med"},{"issue":"3","key":"pntd.0009336.ref032","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1016\/j.jinf.2017.05.021","article-title":"Comparison of Zika virus (ZIKV) RNA detection in plasma, whole blood and urine\u2014Case series of travel-associated ZIKV infection imported to Italy, 2016","volume":"75","author":"G Rossini","year":"2017","journal-title":"J Infect"},{"issue":"5","key":"pntd.0009336.ref033","doi-asserted-by":"crossref","DOI":"10.3201\/eid2405.171747","article-title":"External Quality Assessment for Zika Virus Molecular Diagnostic Testing, Brazil","volume":"24","author":"C Fischer","year":"2018","journal-title":"Emerg Infect Dis"},{"issue":"8","key":"pntd.0009336.ref034","doi-asserted-by":"crossref","first-page":"1232","DOI":"10.3201\/eid1408.080287","article-title":"Genetic and serologic properties of Zika virus associated with an epidemic, Yap State, Micronesia, 2007","volume":"14","author":"RS Lanciotti","year":"2008","journal-title":"Emerg Infect Dis"},{"issue":"12 Suppl","key":"pntd.0009336.ref035","doi-asserted-by":"crossref","first-page":"S30","DOI":"10.1038\/nrmicro2459","article-title":"Evaluation of diagnostic tests: dengue","volume":"8","author":"RW Peeling","year":"2010","journal-title":"Nat Rev Microbiol"},{"issue":"21","key":"pntd.0009336.ref036","doi-asserted-by":"crossref","first-page":"543","DOI":"10.15585\/mmwr.mm6521e1","article-title":"Interim Guidance for Interpretation of Zika Virus Antibody Test Results","volume":"65","author":"IB Rabe","year":"2016","journal-title":"MMWR Morb Mortal Wkly Rep"},{"issue":"4","key":"pntd.0009336.ref037","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.01972-17","article-title":"Diagnostic Testing for Zika Virus: a Postoutbreak Update","volume":"56","author":"ES Theel","year":"2018","journal-title":"J Clin Microbiol"},{"issue":"1","key":"pntd.0009336.ref038","doi-asserted-by":"crossref","first-page":"ofy352","DOI":"10.1093\/ofid\/ofy352","article-title":"Clinical, Virologic, and Immunologic Characteristics of Zika Virus Infection in a Cohort of US Patients: Prolonged RNA Detection in Whole Blood","volume":"6","author":"HM El Sahly","year":"2019","journal-title":"Open Forum Infect Dis"},{"key":"pntd.0009336.ref039","article-title":"Kinetics of anti-Zika virus antibodies after acute infection in pregnant women","author":"B Hoen","year":"2019","journal-title":"J Clin Microbiol"},{"issue":"1","key":"pntd.0009336.ref040","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1186\/s12985-017-0740-6","article-title":"Viral and serological kinetics in Zika virus-infected patients in South Korea","volume":"14","author":"YE Jeong","year":"2017","journal-title":"Virol J"},{"issue":"6","key":"pntd.0009336.ref041","doi-asserted-by":"crossref","first-page":"1894","DOI":"10.1128\/JCM.00346-17","article-title":"Sensitivity and Kinetics of an NS1-Based Zika Virus Enzyme-Linked Immunosorbent Assay in Zika Virus-Infected Travelers from Israel, the Czech Republic, Italy, Belgium, Germany, and Chile","volume":"55","author":"Y Lustig","year":"2017","journal-title":"J Clin Microbiol"},{"issue":"5","key":"pntd.0009336.ref042","doi-asserted-by":"crossref","first-page":"872","DOI":"10.1002\/jmv.25366","article-title":"Kinetics of antigen-specific IgM\/IgG\/IgA antibody responses during Zika virus natural infection in two patients","volume":"91","author":"LZ Zhao","year":"2019","journal-title":"J Med Virol"},{"issue":"2","key":"pntd.0009336.ref043","doi-asserted-by":"crossref","first-page":"299","DOI":"10.3201\/eid2502.181286","article-title":"Zika Virus IgM Detection and Neutralizing Antibody Profiles 12\u201319 Months after Illness Onset","volume":"25","author":"I Griffin","year":"2019","journal-title":"Emerg Infect Dis"},{"issue":"12","key":"pntd.0009336.ref044","doi-asserted-by":"crossref","first-page":"2264","DOI":"10.3201\/eid2512.191022","article-title":"Zika Virus IgM 25 Months after Symptom Onset, Miami-Dade County, Florida, USA","volume":"25","author":"I Griffin","year":"2019","journal-title":"Emerg Infect Dis"},{"key":"pntd.0009336.ref045","first-page":"9","article-title":"Zika seroprevalence declines and neutralizing antibodies wane in adults following outbreaks in French Polynesia and Fiji","author":"AD Henderson","year":"2020","journal-title":"Elife"},{"issue":"5","key":"pntd.0009336.ref046","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1007\/s11262-020-01772-2","article-title":"Rapid decline of Zika virus NS1 antigen-specific antibody responses, northeastern Brazil","volume":"56","author":"A Moreira-Soto","year":"2020","journal-title":"Virus Genes"},{"issue":"5","key":"pntd.0009336.ref047","doi-asserted-by":"crossref","first-page":"773","DOI":"10.3201\/eid2305.161630","article-title":"Lack of Durable Cross-Neutralizing Antibodies Against Zika Virus from Dengue Virus Infection","volume":"23","author":"MH Collins","year":"2017","journal-title":"Emerg Infect Dis"},{"issue":"8","key":"pntd.0009336.ref048","doi-asserted-by":"crossref","first-page":"1485","DOI":"10.3201\/eid2508.190113","article-title":"Cross-Protection of Dengue Virus Infection against Congenital Zika Syndrome, Northeastern Brazil","volume":"25","author":"C Pedroso","year":"2019","journal-title":"Emerg Infect Dis"},{"issue":"50","key":"pntd.0009336.ref049","doi-asserted-by":"crossref","DOI":"10.2807\/1560-7917.ES.2016.21.50.30426","article-title":"Serodiagnosis of Zika virus (ZIKV) infections by a novel NS1-based ELISA devoid of cross-reactivity with dengue virus antibodies: a multicohort study of assay performance, 2015 to 2016","volume":"21","author":"K Steinhagen","year":"2016","journal-title":"Euro Surveill"},{"issue":"2","key":"pntd.0009336.ref050","doi-asserted-by":"crossref","first-page":"311","DOI":"10.3201\/eid2502.180166","article-title":"Differential Shedding and Antibody Kinetics of Zika and Chikungunya Viruses, Brazil","volume":"25","author":"FA Bozza","year":"2019","journal-title":"Emerg Infect Dis"},{"key":"pntd.0009336.ref051","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.jviromet.2019.02.005","article-title":"Added value of IgA antibodies against Zika virus non-structural protein 1 in the diagnosis of acute Zika virus infections","volume":"267","author":"JM Warnecke","year":"2019","journal-title":"J Virol Methods"},{"issue":"2","key":"pntd.0009336.ref052","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1128\/CDLI.10.2.317-322.2003","article-title":"Diagnosis of dengue virus infection by detection of specific immunoglobulin M (IgM) and IgA antibodies in serum and saliva","volume":"10","author":"A Balmaseda","year":"2003","journal-title":"Clin Diagn Lab Immunol"},{"issue":"13","key":"pntd.0009336.ref053","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1016\/S1286-4579(99)00208-7","article-title":"Diagnostic value of dengue virus-specific IgA and IgM serum antibody detection","volume":"1","author":"J Groen","year":"1999","journal-title":"Microbes Infect"},{"key":"pntd.0009336.ref054","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1186\/s12879-016-1440-3","article-title":"Clinical evaluation of dengue and identification of risk factors for severe disease: protocol for a multicentre study in 8 countries","volume":"16","author":"T Jaenisch","year":"2016","journal-title":"BMC Infect Dis"},{"key":"pntd.0009336.ref055","unstructured":"WHO. Situation Report-Zika virus, microcephaly and Guillain-Barr\u00e9 syndrome 2016 [Available from: http:\/\/origin.searo.who.int\/entity\/bhutan\/who-zika-28-7-16.pdf."},{"issue":"suppl 5","key":"pntd.0009336.ref056","doi-asserted-by":"crossref","first-page":"S441","DOI":"10.1093\/infdis\/jiw390","article-title":"Epidemiology of Chikungunya in the Americas","volume":"214","author":"S Yactayo","year":"2016","journal-title":"J Infect Dis"},{"issue":"11","key":"pntd.0009336.ref057","doi-asserted-by":"crossref","first-page":"e0006055","DOI":"10.1371\/journal.pntd.0006055","article-title":"Zika virus displacement by a chikungunya outbreak in Recife, Brazil","volume":"11","author":"T Magalhaes","year":"2017","journal-title":"PLoS Negl Trop Dis"},{"issue":"5","key":"pntd.0009336.ref058","doi-asserted-by":"crossref","first-page":"764","DOI":"10.3201\/eid1305.070015","article-title":"Chikungunya virus in US travelers returning from India, 2006","volume":"13","author":"RS Lanciotti","year":"2007","journal-title":"Emerg Infect Dis"},{"issue":"2","key":"pntd.0009336.ref059","doi-asserted-by":"crossref","first-page":"e1002548","DOI":"10.1371\/journal.ppat.1002548","article-title":"The relative importance of innate immune priming in Wolbachia-mediated dengue interference","volume":"8","author":"E Rances","year":"2012","journal-title":"PLoS Pathog"},{"issue":"4","key":"pntd.0009336.ref060","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1002\/jmv.2176","article-title":"Single rapid TaqMan fluorogenic probe based PCR assay that detects all four dengue serotypes","volume":"66","author":"D Warrilow","year":"2002","journal-title":"J Med Virol"},{"issue":"4","key":"pntd.0009336.ref061","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1017\/S0950268805005753","article-title":"Specific IgM and IgG responses in primary and secondary dengue virus infections determined by enzyme-linked immunosorbent assay","volume":"134","author":"A Sa-Ngasang","year":"2006","journal-title":"Epidemiol Infect"},{"issue":"1","key":"pntd.0009336.ref062","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1186\/s12985-018-1015-6","article-title":"Diagnostic performance of commercial IgM and IgG enzyme-linked immunoassays (ELISAs) for diagnosis of Zika virus infection","volume":"15","author":"M Kikuti","year":"2018","journal-title":"Virol J"},{"issue":"6","key":"pntd.0009336.ref063","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.3201\/eid2506.180361","article-title":"Performance of 2 Commercial Serologic Tests for Diagnosing Zika Virus Infection","volume":"25","author":"S Matheus","year":"2019","journal-title":"Emerg Infect Dis"},{"issue":"1","key":"pntd.0009336.ref064","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.diagmicrobio.2017.09.001","article-title":"Kinetics of anti-ZIKV antibodies after Zika infection using two commercial enzyme-linked immunoassays","volume":"90","author":"C Pasquier","year":"2018","journal-title":"Diagn Microbiol Infect Dis"},{"issue":"3","key":"pntd.0009336.ref065","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/j.diagmicrobio.2017.04.002","article-title":"Evaluation of a commercially available Zika virus IgM ELISA: specificity in focus","volume":"88","author":"K Kadkhoda","year":"2017","journal-title":"Diagn Microbiol Infect Dis"},{"key":"pntd.0009336.ref066","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.jviromet.2018.06.018","article-title":"Multi-laboratory comparison of three commercially available Zika IgM enzyme-linked immunosorbent assays","volume":"260","author":"AJ Basile","year":"2018","journal-title":"J Virol Methods"},{"issue":"8","key":"pntd.0009336.ref067","doi-asserted-by":"crossref","first-page":"2462","DOI":"10.1128\/JCM.00442-17","article-title":"Evaluation of Euroimmun Anti-Zika Virus IgM and IgG Enzyme-Linked Immunosorbent Assays for Zika Virus Serologic Testing","volume":"55","author":"AG L\u2019Huillier","year":"2017","journal-title":"J Clin Microbiol"},{"issue":"2","key":"pntd.0009336.ref068","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.diagmicrobio.2019.01.004","article-title":"Establishment of a comprehensive and high throughput serological algorithm for Zika virus diagnostic testing","volume":"94","author":"EJ Mendoza","year":"2019","journal-title":"Diagn Microbiol Infect Dis"}],"updated-by":[{"DOI":"10.1371\/journal.pntd.0009336","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2021,4,29]],"date-time":"2021-04-29T00:00:00Z","timestamp":1619654400000}}],"container-title":["PLOS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0009336","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,4,29]],"date-time":"2021-04-29T18:03:46Z","timestamp":1619719426000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0009336"}},"subtitle":[],"editor":[{"given":"William B.","family":"Messer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2021,4,19]]},"references-count":68,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2021,4,19]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0009336","relation":{"new_version":[{"id-type":"doi","id":"10.1371\/journal.pntd.0009336","asserted-by":"object"}]},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4,19]]}}}