{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T13:38:52Z","timestamp":1774359532423,"version":"3.50.1"},"reference-count":44,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2021,6,11]],"date-time":"2021-06-11T00:00:00Z","timestamp":1623369600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AI089992"],"award-info":[{"award-number":["AI089992"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AI127865"],"award-info":[{"award-number":["AI127865"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000011","name":"Howard Hughes Medical Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000011","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>\n                    Dengue virus (DENV) is a flavivirus that causes marked human morbidity and mortality worldwide, and is transmitted to humans by\n                    <jats:italic>Aedes aegypti<\/jats:italic>\n                    mosquitoes. Habitat expansion of\n                    <jats:italic>Aedes<\/jats:italic>\n                    , mainly due to climate change and increasing overlap between urban and wild habitats, places nearly half of the world\u2019s population at risk for DENV infection. After a bloodmeal from a DENV-infected host, the virus enters the mosquito midgut. Next, the virus migrates to, and replicates in, other tissues, like salivary glands. Successful viral transmission occurs when the infected mosquito takes another blood meal on a susceptible host and DENV is released from the salivary gland via saliva into the skin. During viral dissemination in the mosquito and transmission to a new mammalian host, DENV interacts with a variety of vector proteins, which are uniquely important during each phase of the viral cycle. Our study focuses on the interaction between DENV particles and protein components in the\n                    <jats:italic>A<\/jats:italic>\n                    .\n                    <jats:italic>aegypti<\/jats:italic>\n                    vector. We performed a mass spectrometry assay where we identified a set of\n                    <jats:italic>A<\/jats:italic>\n                    .\n                    <jats:italic>aegypti<\/jats:italic>\n                    salivary gland proteins which potentially interact with the DENV virion. Using dsRNA to silence gene expression, we analyzed the role of these proteins in viral infectivity. Two of these candidates, a synaptosomal-associated protein (AeSNAP) and a calcium transporter ATPase (ATPase) appear to play a role in viral replication both\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    , observing a ubiquitous expression of these proteins in the mosquito. These findings suggest that AeSNAP plays a protective role during DENV infection of mosquitoes and that ATPase protein is required for DENV during amplification within the vector.\n                  <\/jats:p>","DOI":"10.1371\/journal.pntd.0009442","type":"journal-article","created":{"date-parts":[[2021,6,11]],"date-time":"2021-06-11T13:24:24Z","timestamp":1623417864000},"page":"e0009442","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":13,"title":["Aedes aegypti SNAP and a calcium transporter ATPase influence dengue virus dissemination"],"prefix":"10.1371","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2840-1722","authenticated-orcid":true,"given":"Alejandro","family":"Marin-Lopez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4905-9348","authenticated-orcid":true,"given":"Junjun","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8494-6124","authenticated-orcid":true,"given":"Yuchen","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongguo","family":"Cao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tyler","family":"MacNeil","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew K.","family":"Hastings","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5884-6047","authenticated-orcid":true,"given":"Erol","family":"Fikrig","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2021,6,11]]},"reference":[{"issue":"4","key":"pntd.0009442.ref001","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1016\/S0188-4409(02)00378-8","article-title":"The global emergence\/resurgence of arboviral diseases as public health problems","volume":"33","author":"DJ Gubler","year":"2002","journal-title":"Arch Med Res"},{"issue":"12","key":"pntd.0009442.ref002","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1016\/S1473-3099(12)70246-3","article-title":"Lessons from malaria control to help meet the rising challenge of dengue","volume":"12","author":"KL Anders","year":"2012","journal-title":"Lancet Infect Dis"},{"issue":"4","key":"pntd.0009442.ref003","doi-asserted-by":"crossref","first-page":"3","DOI":"10.2149\/tmh.2011-S05","article-title":"Dengue, Urbanization and Globalization: The Unholy Trinity of the 21(st) Century","volume":"39","author":"DJ Gubler","year":"2011","journal-title":"Trop Med Health"},{"issue":"4","key":"pntd.0009442.ref004","doi-asserted-by":"crossref","first-page":"17","DOI":"10.2149\/tmh.2011-S04","article-title":"Dengue Vectors and their Spatial Distribution","volume":"39","author":"Y. 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