{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T07:28:51Z","timestamp":1790494131054,"version":"4.1.0"},"reference-count":72,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2021,8,17]],"date-time":"2021-08-17T00:00:00Z","timestamp":1629158400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>\n                    Visceral leishmaniasis is a protozoan disease associated with high fatality rate in developing countries. Although the drug pipeline is constantly improving, available treatments are costly and live-threatening side effects are not uncommon. Moreover, an approved vaccine against human leishmaniasis does not exist yet. Using whole antigens from\n                    <jats:italic>Leishmania donovani<\/jats:italic>\n                    promastigotes (LdAg), we investigated the protective potential of a novel adjuvant-free vaccine strategy. Immunization of mice with LdAg\n                    <jats:italic>via<\/jats:italic>\n                    the intradermal or the intranasal route prior to infection decreases the parasitic burden in primary affected internal organs, including the liver, spleen, and bone marrow. Interestingly, the intranasal route is more efficient than the intradermal route, leading to better parasite clearance and remarkable induction of adaptive immune cells, notably the helper and cytotoxic T cells.\n                    <jats:italic>In vitro<\/jats:italic>\n                    restimulation experiments with\n                    <jats:italic>Leishmania<\/jats:italic>\n                    antigens led to significant IFN-\u03b3 secretion by splenocytes; therefore, exemplifying specificity of the adaptive immune response. To improve mucosal delivery and the immunogenic aspects of our vaccine strategy, we used polysaccharide-based nanoparticles (NP) that carry the antigens. The NP-LdAg formulation is remarkably taken up by dendritic cells and induces their maturation\n                    <jats:italic>in vitro<\/jats:italic>\n                    , as revealed by the increased expression of CD80, CD86 and MHC II. Intranasal immunization with NP-LdAg does not improve the parasite clearance in our experimental timeline; however, it does increase the percentage of effector and memory T helper cells in the spleen, suggesting a potential induction of long-term memory. Altogether, this study provides a simple and cost-effective vaccine strategy against visceral leishmaniasis based on LdAg administration\n                    <jats:italic>via<\/jats:italic>\n                    the intranasal route, which could be applicable to other parasitic diseases.\n                  <\/jats:p>","DOI":"10.1371\/journal.pntd.0009627","type":"journal-article","created":{"date-parts":[[2021,8,17]],"date-time":"2021-08-17T14:29:14Z","timestamp":1629210554000},"page":"e0009627","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":21,"title":["Intranasal vaccine from whole Leishmania donovani antigens provides protection and induces specific immune response against visceral leishmaniasis"],"prefix":"10.1371","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5835-9214","authenticated-orcid":true,"given":"Doumet Georges","family":"Helou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2975-9275","authenticated-orcid":true,"given":"Aur\u00e9lie","family":"Mauras","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Fasquelle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7290-3263","authenticated-orcid":true,"given":"Juliane Sousa","family":"Lanza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8542-6793","authenticated-orcid":true,"given":"Philippe M.","family":"Loiseau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"Betbeder","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandrine","family":"Cojean","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,8,17]]},"reference":[{"key":"pntd.0009627.ref001","unstructured":"Leishmaniasis. 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Dewitte","year":"2014","journal-title":"J Control Release"},{"key":"pntd.0009627.ref046","doi-asserted-by":"crossref","DOI":"10.1155\/2018\/3495086","article-title":"Optimization of Ex Vivo Murine Bone Marrow Derived Immature Dendritic Cells: A Comparative Analysis of Flask Culture Method and Mouse CD11c Positive Selection Kit Method.","volume":"2018","author":"RA Gosavi","year":"2018","journal-title":"Bone Marrow Res."},{"key":"pntd.0009627.ref047","doi-asserted-by":"crossref","first-page":"1323","DOI":"10.4161\/hv.21350","article-title":"Dendritic cell-based in vitro assays for vaccine immunogenicity","volume":"8","author":"RJ Vandebriel","year":"2012","journal-title":"Hum Vaccin Immunother"},{"key":"pntd.0009627.ref048","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1038\/nature01004","article-title":"T-cell engagement of dendritic cells rapidly rearranges MHC class II transport","volume":"418","author":"M Boes","year":"2002","journal-title":"Nature"},{"key":"pntd.0009627.ref049","doi-asserted-by":"crossref","first-page":"8820","DOI":"10.1073\/pnas.1202977109","article-title":"MHC class II distribution in dendritic cells and B cells is determined by ubiquitin chain length","volume":"109","author":"JK Ma","year":"2012","journal-title":"Proc Natl Acad Sci U S A"},{"key":"pntd.0009627.ref050","doi-asserted-by":"crossref","first-page":"4521","DOI":"10.1128\/IAI.72.8.4521-4527.2004","article-title":"Intranasal Vaccination against Cutaneous Leishmaniasis with a Particulated Leishmanial Antigen or DNA Encoding LACK","volume":"72","author":"EF Pinto","year":"2004","journal-title":"Infection and Immunity"},{"key":"pntd.0009627.ref051","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1590\/0037-8682-0120-2016","article-title":"Recent updates and perspectives on approaches for the development of vaccines against visceral leishmaniasis","volume":"49","author":"MC Duarte","year":"2016","journal-title":"Rev Soc Bras Med Trop"},{"key":"pntd.0009627.ref052","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1007\/s12639-018-0986-y","article-title":"Using proteomics as a powerful tool to develop a vaccine against Mediterranean visceral leishmaniasis","volume":"42","author":"S Rashidi","year":"2018","journal-title":"J Parasit Dis"},{"key":"pntd.0009627.ref053","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1186\/s13071-020-04064-8","article-title":"An immunoinformatic approach driven by experimental proteomics: in silico design of a subunit candidate vaccine targeting secretory proteins of Leishmania donovani amastigotes.","volume":"13","author":"MAA Khan","year":"2020","journal-title":"Parasites & Vectors."},{"key":"pntd.0009627.ref054","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1590\/S0037-86822003000200001","article-title":"Identification and purification of immunogenic proteins from nonliving promastigote polyvalent Leishmania vaccine (Leishvacin).","volume":"36","author":"SRA Cardoso","year":"2003","journal-title":"Rev Soc Bras Med Trop."},{"key":"pntd.0009627.ref055","article-title":"Antigenicity, Immunogenicity and Protective Efficacy of Three Proteins Expressed in the Promastigote and Amastigote Stages of Leishmania infantum against Visceral Leishmaniasis","volume":"10","author":"VT Martins","year":"2015","journal-title":"PLoS One"},{"key":"pntd.0009627.ref056","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1186\/s13071-020-04138-7","article-title":"Investigation of the antigenicity and protective efficacy of Leishmania promastigote membrane antigens in search of potential diagnostic and vaccine candidates against visceral leishmaniasis.","volume":"13","author":"SA Ejazi","year":"2020","journal-title":"Parasites & Vectors."},{"key":"pntd.0009627.ref057","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1038\/s41598-019-57245-6","article-title":"Intranasal 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