{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T02:11:00Z","timestamp":1792289460991,"version":"4.4.0"},"update-to":[{"DOI":"10.1371\/journal.pntd.0009793","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2022,5,5]],"date-time":"2022-05-05T00:00:00Z","timestamp":1651708800000}}],"reference-count":18,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2022,4,25]],"date-time":"2022-04-25T00:00:00Z","timestamp":1650844800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP20fk0108138"],"award-info":[{"award-number":["JP20fk0108138"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012319","name":"National Center for Global Health and Medicine","doi-asserted-by":"crossref","award":["21A1002"],"award-info":[{"award-number":["21A1002"]}],"id":[{"id":"10.13039\/100012319","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Background<\/jats:title>\n<jats:p>Amebiasis, caused by <jats:italic>Entamoeba histolytica<\/jats:italic>, is spreading in developing countries and in many developed countries as a sexually transmitted infection. Here, we evaluated the efficacy of serological screening to identify asymptomatic <jats:italic>E<\/jats:italic>. <jats:italic>histolytica<\/jats:italic> infection as a potential epidemiological control measure to limit its spread.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methodology\/Principal findings<\/jats:title>\n<jats:p>This cross-sectional study was carried out between January and March 2021 in an HIV-negative men who have sex with men (MSM) cohort at the National Center for Global Health and Medicine. Serological screening was performed using a commercially available ELISA kit. For seropositive individuals, we performed stool polymerase chain reaction (PCR) to determine current <jats:italic>E<\/jats:italic>. <jats:italic>histolytica<\/jats:italic> infection. We performed <jats:italic>E<\/jats:italic>. <jats:italic>histolytica<\/jats:italic> serological screening of 312 participants. None had a history of <jats:italic>E<\/jats:italic>. <jats:italic>histolytica<\/jats:italic> infection prior to the study. The overall <jats:italic>E<\/jats:italic>. <jats:italic>histolytica<\/jats:italic> seropositivity was 6.7% (21\/312), which was similar to that found by the rapid plasma reagin test (17\/312). We identified current infection in 8 of 20 seropositive participants (40.0%) by stool PCR.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Conclusions\/Significance<\/jats:title>\n<jats:p>Our serological screening approach constitutes a potentially practical epidemiological strategy. Active epidemiological surveys, in combination with an effective screening strategy for asymptomatically infected individuals, should be applied to help reduce sexually transmitted <jats:italic>E<\/jats:italic>. <jats:italic>histolytica<\/jats:italic> infections.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pntd.0009793","type":"journal-article","created":{"date-parts":[[2022,4,25]],"date-time":"2022-04-25T17:33:44Z","timestamp":1650908024000},"page":"e0009793","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":15,"title":["Identification of asymptomatic Entamoeba histolytica infection by a serological screening test: A cross-sectional study of an HIV-negative men who have sex with men cohort in Japan"],"prefix":"10.1371","volume":"16","author":[{"given":"Yasuaki","family":"Yanagawa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rieko","family":"Shimogawara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5059-110X","authenticated-orcid":true,"given":"Misao","family":"Takano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takahiro","family":"Aoki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisuke","family":"Mizushima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Gatanaga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshimi","family":"Kikuchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9283-2167","authenticated-orcid":true,"given":"Shinichi","family":"Oka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenji","family":"Yagita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3190-8439","authenticated-orcid":true,"given":"Koji","family":"Watanabe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,4,25]]},"reference":[{"issue":"9859","key":"pntd.0009793.ref001","doi-asserted-by":"crossref","first-page":"2095","DOI":"10.1016\/S0140-6736(12)61728-0","article-title":"Global and regional mortality from 235 causes of death for 20 age groups in 1990 and 2010: a systematic analysis for the Global Burden of Disease Study 2010","volume":"380","author":"R Lozano","year":"2012","journal-title":"Lancet"},{"issue":"5","key":"pntd.0009793.ref002","doi-asserted-by":"crossref","first-page":"e228942","DOI":"10.1136\/bcr-2018-228942","article-title":"An underestimated sexually transmitted infection: amoebiasis","volume":"12","author":"AC Billet","year":"2019","journal-title":"BMJ Case Rep"},{"issue":"9","key":"pntd.0009793.ref003","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1016\/S1473-3099(12)70147-0","article-title":"Entamoeba histolytica infection in men who have sex with men","volume":"12","author":"CC Hung","year":"2012","journal-title":"Lancet Infect Dis"},{"issue":"30","key":"pntd.0009793.ref004","doi-asserted-by":"crossref","first-page":"30581","DOI":"10.2807\/1560-7917.ES.2017.22.30.30581","article-title":"Outbreak of intestinal amoebiasis among men who have sex with men, Barcelona (Spain), October 2016 and January 2017","volume":"22","author":"L Escola-Verge","year":"2017","journal-title":"Euro Surveill"},{"key":"pntd.0009793.ref005","doi-asserted-by":"crossref","first-page":"1008","DOI":"10.4269\/ajtmh.15-0560","article-title":"Epidemiology of Domestically Acquired Amebiasis in Japan, 2000\u20132013","volume":"94","author":"M Ishikane","year":"2016","journal-title":"Am J Trop Med Hyg"},{"key":"pntd.0009793.ref006","doi-asserted-by":"crossref","first-page":"266","DOI":"10.7883\/yoken.JJID.2015.420","article-title":"A strong correlation between the annual incidence of amebiasis and homosexual human immunodeficiency virus type infection in men","volume":"69","author":"H. 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