{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T21:15:10Z","timestamp":1792271710523,"version":"4.4.0"},"update-to":[{"DOI":"10.1371\/journal.pntd.0009815","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2021,10,12]],"date-time":"2021-10-12T00:00:00Z","timestamp":1633996800000}}],"reference-count":112,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2021,9,30]],"date-time":"2021-09-30T00:00:00Z","timestamp":1632960000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI095491"],"award-info":[{"award-number":["R01AI095491"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Colciencias (minciencias.gov.co), Universidad de Antioquia and Max Planck Society cooperation grant","award":["566-1-2014"],"award-info":[{"award-number":["566-1-2014"]}]},{"name":"Cornell University Graduate School","award":["Sage Fellowship"],"award-info":[{"award-number":["Sage Fellowship"]}]},{"name":"Cornell University Einaudi Center","award":["International Research Travel Grant"],"award-info":[{"award-number":["International Research Travel Grant"]}]},{"DOI":"10.13039\/100000061","name":"Fogarty International Center","doi-asserted-by":"publisher","award":["2D43TW007120-11A1"],"award-info":[{"award-number":["2D43TW007120-11A1"]}],"id":[{"id":"10.13039\/100000061","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:sec id=\"sec001\"><jats:title>Background<\/jats:title><jats:p><jats:italic>Aedes aegypti<\/jats:italic>mosquitoes are globally distributed vectors of viruses that impact the health of hundreds of millions of people annually. Mating and blood feeding represent fundamental aspects of mosquito life history that carry important implications for vectorial capacity and for control strategies. Females transmit pathogens to vertebrate hosts and obtain essential nutrients for eggs during blood feeding. Further, because host-seeking<jats:italic>Ae<\/jats:italic>.<jats:italic>aegypti<\/jats:italic>females mate with males swarming near hosts, biological crosstalk between these behaviors could be important. Although mating influences nutritional intake in other insects, prior studies examining mating effects on mosquito blood feeding have yielded conflicting results.<\/jats:p><\/jats:sec><jats:sec id=\"sec002\"><jats:title>Methodology\/Principal findings<\/jats:title><jats:p>To resolve these discrepancies, we examined blood-feeding physiology and behavior in virgin and mated females and in virgins injected with male accessory gland extracts (MAG), which induce post-mating changes in female behavior. We controlled adult nutritional status prior to blood feeding by using water- and sugar-fed controls. Our data show that neither mating nor injection with MAG affect<jats:italic>Ae<\/jats:italic>.<jats:italic>aegypti<\/jats:italic>blood intake, digestion, or feeding avidity for an initial blood meal. However, sugar feeding, a common supplement in laboratory settings but relatively rare in nature, significantly affected all aspects of feeding and may have contributed to conflicting results among previous studies. Further, mating, MAG injection, and sugar intake induced declines in subsequent feedings after an initial blood meal, correlating with egg production and laying. Taking our evaluation to the field, virgin and mated mosquitoes collected in Colombia were equally likely to contain blood at the time of collection.<\/jats:p><\/jats:sec><jats:sec id=\"sec003\"><jats:title>Conclusions\/Significance<\/jats:title><jats:p>Mating, MAG, and sugar feeding impact a mosquito\u2019s estimated ability to transmit pathogens through both direct and indirect effects on multiple aspects of mosquito biology. Our results highlight the need to consider natural mosquito ecology, including diet, when assessing their physiology and behavior in the laboratory.<\/jats:p><\/jats:sec>","DOI":"10.1371\/journal.pntd.0009815","type":"journal-article","created":{"date-parts":[[2021,9,30]],"date-time":"2021-09-30T19:47:02Z","timestamp":1633031222000},"page":"e0009815","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":56,"title":["The impact of mating and sugar feeding on blood-feeding physiology and behavior in the arbovirus vector mosquito Aedes aegypti"],"prefix":"10.1371","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1259-3857","authenticated-orcid":true,"given":"Garrett P.","family":"League","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3853-9151","authenticated-orcid":true,"given":"Ethan 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dengue","volume":"496","author":"S Bhatt","year":"2013","journal-title":"Nature"},{"key":"pntd.0009815.ref003","doi-asserted-by":"crossref","first-page":"894","DOI":"10.1126\/science.aat7115","article-title":"Genomic and epidemiological monitoring of yellow fever virus transmission potential","volume":"361","author":"NR Faria","year":"2018","journal-title":"Science"},{"key":"pntd.0009815.ref004","doi-asserted-by":"crossref","first-page":"e0005625","DOI":"10.1371\/journal.pntd.0005625","article-title":"Contemporary status of insecticide resistance in the major Aedes vectors of arboviruses infecting humans.","volume":"11","author":"CL Moyes","year":"2017","journal-title":"PLoS Negl Trop Dis"},{"key":"pntd.0009815.ref005","doi-asserted-by":"crossref","first-page":"e08347","DOI":"10.7554\/eLife.08347","article-title":"The global distribution of the arbovirus vectors Aedes aegypti and Ae. albopictus.","volume":"4","author":"MU Kraemer","year":"2015","journal-title":"Elife"},{"key":"pntd.0009815.ref006","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1146\/annurev-med-050715-105122","article-title":"Zika, chikungunya, and other emerging vector-borne viral diseases.","volume":"69","author":"SC Weaver","year":"2018","journal-title":"Annu Rev Med"},{"key":"pntd.0009815.ref007","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.jinsphys.2015.08.001","article-title":"Multiple factors contribute to anautogenous reproduction by the mosquito Aedes aegypti","volume":"82","author":"M Gulia-Nuss","year":"2015","journal-title":"J Insect Physiol"},{"key":"pntd.0009815.ref008","doi-asserted-by":"crossref","first-page":"780","DOI":"10.4269\/ajtmh.15-0893","article-title":"Polyandry depends on postmating time interval in the dengue vector Aedes aegypti","volume":"94","author":"EC Degner","year":"2016","journal-title":"Am J Trop Med 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I. Preliminary studies of physiological control mechanisms.","volume":"133","author":"CL Judson","year":"1967","journal-title":"Biol Bull"},{"key":"pntd.0009815.ref020","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1017\/S0007485300030881","article-title":"Variation in the hatching-response of Aedes eggs (Diptera: Culicidae).","volume":"46","author":"JD Gillett","year":"1955","journal-title":"Bull Entomol Res"},{"key":"pntd.0009815.ref021","first-page":"448","article-title":"Accessory gland substance as a stimulant for oviposition in Aedes aegypti and A. albopictus","volume":"25","author":"MG Leahy","year":"1965","journal-title":"Mosq News"},{"key":"pntd.0009815.ref022","doi-asserted-by":"crossref","first-page":"2217","DOI":"10.1016\/0022-1910(72)90250-8","article-title":"The effect of matrone on oviposition in the mosquito, Aedes aegypti","volume":"18","author":"EA Hiss","year":"1972","journal-title":"J Insect Physiol"},{"key":"pntd.0009815.ref023","doi-asserted-by":"crossref","first-page":"955","DOI":"10.4039\/Ent108955-9","article-title":"Functions of the male accessory gland secretions of Aedes mosquitoes (Diptera: Culicidae): Transplantation studies.","volume":"108","author":"S Ramalingam","year":"1976","journal-title":"Can Entomol"},{"key":"pntd.0009815.ref024","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1177\/16.12.765","article-title":"Nonspecific esterases in moquitos","volume":"16","author":"TA Freyvogel","year":"1968","journal-title":"J Histochem Cytochem"},{"key":"pntd.0009815.ref025","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1016\/0022-1910(91)90105-9","article-title":"Male accessory gland substances activate egg development in nutritionally stressed Aedes aegypti mosquitoes","volume":"37","author":"MJ Klowden","year":"1991","journal-title":"J Insect Physiol"},{"key":"pntd.0009815.ref026","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1093\/jmedent\/29.3.467","article-title":"Reproductive and metabolic differences between Aedes aegypti and Ae. albopictus (Diptera: Culicidae).","volume":"29","author":"MJ Klowden","year":"1992","journal-title":"J Med Entomol"},{"key":"pntd.0009815.ref027","doi-asserted-by":"crossref","first-page":"R196","DOI":"10.1016\/j.cub.2019.02.005","article-title":"Male accessory gland molecules inhibit harmonic convergence in the mosquito Aedes aegypti","volume":"29","author":"GP League","year":"2019","journal-title":"Curr Biol"},{"key":"pntd.0009815.ref028","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1111\/j.1365-3032.1981.tb00275.x","article-title":"The programming of circadian flight-activity in relation to mating and the gonotrophic cycle in the mosquito, Aedes aegypti","volume":"6","author":"MDR Jones","year":"1981","journal-title":"Physiol Entomol"},{"key":"pntd.0009815.ref029","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1111\/j.1365-3032.1989.tb00939.x","article-title":"Changes in the circadian flight activity of the mosquito Anopheles stephensi associated with insemination, blood-feeding, oviposition and nocturnal light intensity","volume":"14","author":"M. Rowland","year":"1989","journal-title":"Physiol Entomol"},{"key":"pntd.0009815.ref030","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1111\/j.1365-3032.1978.tb00151.x","article-title":"Changes in the circadian flight activity of the mosquito Anopheles gambiae in relation to insemination, feeding and oviposition","volume":"3","author":"MDR Jones","year":"1978","journal-title":"Physiol Entomol"},{"key":"pntd.0009815.ref031","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1111\/j.1365-3032.1979.tb00626.x","article-title":"Modification of female circadian flight-activity by a male accessory gland pheromone in the mosquito, Culex pipiens quinquefasciatus","volume":"4","author":"MDR Jones","year":"1979","journal-title":"Physiol Entomol"},{"key":"pntd.0009815.ref032","first-page":"895","article-title":"Insemination-dependent modification of circadian activity of the mosquito, Culex pipiens pallens","volume":"7","author":"Y Chiba","year":"1990","journal-title":"Zool Sci"},{"key":"pntd.0009815.ref033","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1111\/j.1365-3032.1992.tb01013.x","article-title":"A comparative study on insemination dependency of circadian activity pattern in mosquitoes","volume":"17","author":"Y Chiba","year":"1992","journal-title":"Physiol Entomol"},{"key":"pntd.0009815.ref034","first-page":"4","article-title":"A male accessory gland protein that modulates female mosquito (Diptera: Culicidae) host-seeking behavior.","volume":"15","author":"J-J Lee","year":"1999","journal-title":"J Am Mosq Control Assoc"},{"key":"pntd.0009815.ref035","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1016\/0022-1910(95)00048-Y","article-title":"Male accessory gland substances modify the host-seeking behavior of gravid Aedes aegypti mosquitoes","volume":"41","author":"NM Fernandez","year":"1995","journal-title":"J Insect Physiol"},{"key":"pntd.0009815.ref036","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/0022-1910(79)90048-9","article-title":"Humoral inhibition of host-seeking in Aedes aegypti during o\u00f6cyte maturation","volume":"25","author":"MJ Klowden","year":"1979","journal-title":"J Insect Physiol"},{"key":"pntd.0009815.ref037","doi-asserted-by":"crossref","first-page":"1781","DOI":"10.1038\/1811781a0","article-title":"Biting behaviour of mated and unmated females of an African strain of Aedes aegypti","volume":"181","author":"MMJ Lavoipierre","year":"1958","journal-title":"Nature"},{"key":"pntd.0009815.ref038","doi-asserted-by":"crossref","first-page":"1567","DOI":"10.1038\/1821567a0","article-title":"Presence of a factor inhibiting biting in Aedes aegypti","volume":"182","author":"MMJ Lavoipierre","year":"1958","journal-title":"Nature"},{"key":"pntd.0009815.ref039","first-page":"847","article-title":"Observations on the mating behaviour of Aedes aegypti in nature","volume":"45","author":"WK Hartberg","year":"1971","journal-title":"Bull World Health Organ"},{"key":"pntd.0009815.ref040","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jinsphys.2018.05.001","article-title":"Male contributions during mating increase female survival in the disease vector mosquito Aedes aegypti","volume":"108","author":"SM Villarreal","year":"2018","journal-title":"J Insect Physiol"},{"key":"pntd.0009815.ref041","doi-asserted-by":"crossref","first-page":"e0004451","DOI":"10.1371\/journal.pntd.0004451","article-title":"Mating-induced transcriptome changes in the reproductive tract of female Aedes aegypti.","volume":"10","author":"C Alfonso-Parra","year":"2016","journal-title":"PLoS Negl Trop Dis"},{"key":"pntd.0009815.ref042","article-title":"Seminal fluid proteins induce transcriptome changes in the Aedes aegypti female lower reproductive tract, including up-regulation of immunity-related RNAs.","author":"IA Amaro","year":"2021","journal-title":"Sci Rep."},{"key":"pntd.0009815.ref043","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1146\/annurev-ento-120709-144823","article-title":"Insect seminal fluid proteins: Identification and function","volume":"56","author":"FW Avila","year":"2011","journal-title":"Annu Rev Entomol"},{"key":"pntd.0009815.ref044","doi-asserted-by":"crossref","first-page":"692","DOI":"10.1016\/j.cub.2006.02.064","article-title":"Allocrine modulation of feeding behavior by the sex peptide of Drosophila","volume":"16","author":"GB Carvalho","year":"2006","journal-title":"Curr Biol"},{"key":"pntd.0009815.ref045","first-page":"277","volume-title":"Vector olfaction and behavior.","author":"MJ Klowden","year":"2005"},{"key":"pntd.0009815.ref046","doi-asserted-by":"crossref","first-page":"S12","DOI":"10.1016\/j.actatropica.2013.11.021","article-title":"Male reproductive biology of Aedes mosquitoes","volume":"132","author":"CF Oliva","year":"2014","journal-title":"Acta Trop"},{"key":"pntd.0009815.ref047","doi-asserted-by":"crossref","DOI":"10.1079\/9780851993133.0000","volume-title":"The Biology of Mosquitoes: Sensory, Reception, and Behaviour","author":"AN Clements","year":"1999"},{"key":"pntd.0009815.ref048","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1603\/ME11019","article-title":"Behavioral observations and sound recordings of free-flight mating swarms of Ae. aegypti (Diptera: Culicidae) in Thailand.","volume":"48","author":"LJ Cator","year":"2011","journal-title":"J Med Entomol"},{"key":"pntd.0009815.ref049","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1016\/j.aspen.2018.12.025","article-title":"Sex before or after blood feeding: Mating activities of Aedes aegypti males under conditions of different densities and female blood feeding opportunities","volume":"22","author":"H Dieng","year":"2019","journal-title":"J Asia Pac Entomol"},{"key":"pntd.0009815.ref050","first-page":"213","article-title":"The check is in the male: Male mosquitoes affect female physiology and behavior.","volume":"15","author":"MJ Klowden","year":"1999","journal-title":"Joumal Am Mosq Control Assoc"},{"key":"pntd.0009815.ref051","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1146\/annurev.ento.48.091801.112657","article-title":"Male accessory gland secretions: Modulators of female reproductive physiology and behavior","volume":"48","author":"C. 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Handel E","year":"1994","journal-title":"J Am Mosq Control Assoc"},{"key":"pntd.0009815.ref071","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1093\/jmedent\/34.6.589","article-title":"Influence of plant abundance on nectar feeding by Aedes aegypti (Diptera: Culicidae) in southern Mexico.","volume":"34","author":"JA Martinez-Ibarra","year":"1997","journal-title":"J Med Entomol"},{"key":"pntd.0009815.ref072","first-page":"234","article-title":"An experimental study on the detection of fructose in Aedes aegypti","volume":"14","author":"A Costero","year":"1998","journal-title":"J Am Mosq Control Assoc"},{"key":"pntd.0009815.ref073","doi-asserted-by":"crossref","first-page":"177","DOI":"10.2987\/8756-971X(2005)21[177:FVITDV]2.0.CO;2","article-title":"Fructose variation in the dengue vector, Aedes aegypti, during high and low transmission seasons in the Mae Sot region of Thailand","volume":"21","author":"CY Spencer","year":"2005","journal-title":"J Am Mosq Control Assoc"},{"key":"pntd.0009815.ref074","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1093\/jme\/tjaa005","article-title":"Sugar feeding patterns for Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae) mosquitoes in South Texas.","volume":"57","author":"MF Olson","year":"2020","journal-title":"J Med Entomol"},{"key":"pntd.0009815.ref075","doi-asserted-by":"crossref","first-page":"235","DOI":"10.4269\/ajtmh.1997.57.235","article-title":"A fitness advantage for Aedes aegypti and the viruses it transmits when females feed only on human blood","volume":"57","author":"TW Scott","year":"1997","journal-title":"Am J Trop Med Hyg"},{"key":"pntd.0009815.ref076","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1603\/0022-2585-38.3.411","article-title":"Why do female Aedes aegypti (Diptera: Culicidae) feed preferentially and frequently on human blood?","volume":"38","author":"LC Harrington","year":"2001","journal-title":"J Med Entomol"},{"key":"pntd.0009815.ref077","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1093\/jmedent\/42.5.844","article-title":"Blood feeding patterns of Aedes aegypti and Aedes albopictus in Thailand","volume":"42","author":"A Ponlawat","year":"2005","journal-title":"J Med Entomol"},{"key":"pntd.0009815.ref078","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1603\/0022-2585-37.1.89","article-title":"Longitudinal studies of Aedes aegypti (Diptera: Culicidae) in Thailand and Puerto Rico: Blood feeding frequency.","volume":"37","author":"TW Scott","year":"2000","journal-title":"J Med Entomol"},{"key":"pntd.0009815.ref079","doi-asserted-by":"crossref","first-page":"716","DOI":"10.1093\/jmedent\/27.4.716","article-title":"Relationship of wing length to adult dry weight in several mosquito species (Diptera: Culicidae).","volume":"27","author":"RS Nasci","year":"1990","journal-title":"J Med Entomol"},{"key":"pntd.0009815.ref080","first-page":"188","volume-title":"Relazione della campagna antianofelica di Fiumicino (1919), con speciale riguardo alla biologia degli anofeli ed agli anofeli infetti (con undici tavole e dieci figure inserite nel testo). Seconda relazione della lotta antimalarica a Fiumicino (Roma).","author":"M Sella","year":"1920"},{"key":"pntd.0009815.ref081","first-page":"13","article-title":"Age-grouping methods in Diptera of medical importance with special reference to some vectors of malaria.","volume":"47","author":"TS Detinova","year":"1962","journal-title":"Monogr Ser World Heal Organ"},{"key":"pntd.0009815.ref082","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH Image to ImageJ: 25 years of image analysis.","volume":"9","author":"CA Schneider","year":"2012","journal-title":"Nat Methods."},{"key":"pntd.0009815.ref083","doi-asserted-by":"crossref","first-page":"e3048","DOI":"10.1371\/journal.pntd.0003048","article-title":"Heterogeneous feeding patterns of the dengue vector, Aedes aegypti, on individual human hosts in rural Thailand.","volume":"8","author":"LC Harrington","year":"2014","journal-title":"PLoS Negl Trop Dis"},{"key":"pntd.0009815.ref084","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1603\/033.046.0602","article-title":"A new, cost-effective, battery-powered aspirator for adult mosquito collections","volume":"46","author":"GM Vazquez-Prokopec","year":"2009","journal-title":"J Med Entomol"},{"key":"pntd.0009815.ref085","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1365-3032.1984.tb00675.x","article-title":"Ovarian development in mosquitoes: Stages of growth and arrest, and follicular resorption","volume":"9","author":"AN Clements","year":"1984","journal-title":"Physiol Entomol"},{"key":"pntd.0009815.ref086","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1038\/s41564-018-0214-7","article-title":"Vector biology meets disease control: Using basic research to fight vector-borne diseases","volume":"4","author":"WR Shaw","year":"2019","journal-title":"Nat Microbiol"},{"key":"pntd.0009815.ref087","first-page":"9","article-title":"Genetic specificity and potential for local adaptation between dengue viruses and mosquito vectors","author":"L Lambrechts","year":"2009","journal-title":"BMC Evol Biol"},{"key":"pntd.0009815.ref088","doi-asserted-by":"crossref","first-page":"689","DOI":"10.4269\/ajtmh.12-0488","article-title":"Reduction of Aedes aegypti vector competence for dengue virus under large temperature fluctuations","volume":"88","author":"LB Carrington","year":"2013","journal-title":"Am J Trop Med Hyg"},{"key":"pntd.0009815.ref089","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.meegid.2018.11.009","article-title":"Aedes aegypti vector competence studies: A review","volume":"67","author":"JA Souza-Neto","year":"2019","journal-title":"Infect Genet Evol"},{"key":"pntd.0009815.ref090","doi-asserted-by":"crossref","first-page":"e2190","DOI":"10.1371\/journal.pntd.0002190","article-title":"Fluctuations at a low mean temperature accelerate dengue virus transmission by Aedes aegypti.","volume":"7","author":"LB Carrington","year":"2013","journal-title":"PLoS Negl Trop Dis."},{"key":"pntd.0009815.ref091","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1603\/0022-2585-38.4.537","article-title":"Analysis of survival of young and old Aedes aegypti (Diptera: Culicidae) from Puerto Rico and Thailand.","volume":"38","author":"LC Harrington","year":"2001","journal-title":"J Med Entomol"},{"key":"pntd.0009815.ref092","doi-asserted-by":"crossref","first-page":"e2207","DOI":"10.1371\/journal.pntd.0002207","article-title":"Extrinsic incubation period of dengue: Knowledge, backlog, and applications of temperature dependence.","volume":"7","author":"NB Tjaden","year":"2013","journal-title":"PLoS Negl Trop Dis"},{"key":"pntd.0009815.ref093","first-page":"40","volume-title":"Power analysis. Handbook of biological statistics","author":"JH McDonald","year":"2014","edition":"3"},{"key":"pntd.0009815.ref094","unstructured":"SPSS library: How do I handle interactions of continuous and categorical variables? In: UCLA: Statistical Consulting Group [Internet]. Available: https:\/\/stats.idre.ucla.edu\/spss\/library\/spss-libraryhow-do-i-handle-interactions-of-continuous-andcategorical-variables\/."},{"key":"pntd.0009815.ref095","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1089\/vbz.2007.0216","article-title":"Mortality and reproductive dynamics of Aedes aegypti (Diptera: Culicidae) fed human blood.","volume":"7","author":"LM Styer","year":"2007","journal-title":"Vector Borne Zoonotic Dis"},{"key":"pntd.0009815.ref096","doi-asserted-by":"crossref","first-page":"909","DOI":"10.4269\/ajtmh.1956.5.909","article-title":"The influence of mating on egg production by Aedes aegypti","volume":"5","author":"CA Lang","year":"1956","journal-title":"Am J Trop Med Hyg"},{"key":"pntd.0009815.ref097","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1093\/jmedent\/5.1.21","article-title":"Physiology of feeding and oviposition behavior in Aedes aegypti (L.): Experimental dissociation of feeding and oogenesis","volume":"5","author":"CL Judson","year":"1968","journal-title":"J Med Entomol"},{"key":"pntd.0009815.ref098","doi-asserted-by":"crossref","first-page":"2009","DOI":"10.1093\/jee\/63.6.2009","article-title":"A study of the probing response of Aedes aegypti III. 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