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Polyvalent antivenom therapy in the management of such envenomation cases is finite due to its poor venom neutralization capabilities as well as diagnostic ramifications manifested as untoward immunological reactions. For precise molecular diagnosis of elapid venoms of the big four snakes, we have developed a lateral flow kit using a monoclonal antibody (AB1; IgG<jats:sub>1<\/jats:sub> \u2013 \u03ba chain; Kd: 31 nM) generated against recombinant cytotoxin-7 (rCTX-7; 7.7 kDa) protein of the elapid venom. The monoclonal antibody specifically detected the venoms of <jats:italic>Naja naja<\/jats:italic> (p &lt; 0.0001) and <jats:italic>Bungarus caeruleus<\/jats:italic> (p&lt;0.0001), without showing any immunoreactivity against the viperidae snakes in big four venomous snakes. The kit developed attained the limit of quantitation of 170 pg\/\u03bcL and 2.1 ng\/\u03bcL in spiked buffer samples and 28.7 ng\/\u03bcL and 110 ng\/\u03bcL in spiked serum samples for detection of <jats:italic>N<\/jats:italic>. <jats:italic>naja<\/jats:italic> and <jats:italic>B<\/jats:italic>. <jats:italic>caeruleus<\/jats:italic> venoms, respectively. This kit holds enormous potential in identification of elapid venom of the big four snakes for effective prognosis of an envenomation; as per the existing medical guidelines.<\/jats:p>","DOI":"10.1371\/journal.pntd.0009841","type":"journal-article","created":{"date-parts":[[2021,10,11]],"date-time":"2021-10-11T21:14:36Z","timestamp":1633986876000},"page":"e0009841","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":11,"title":["Cytotoxin antibody-based colourimetric sensor for field-level differential detection of elapid among big four snake venom"],"prefix":"10.1371","volume":"15","author":[{"given":"Sherin","family":"Kaul","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Sai Keerthana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0500-0256","authenticated-orcid":true,"given":"Pankaj","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2810-7224","authenticated-orcid":true,"given":"Komal","family":"Birader","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yathirajarao","family":"Tammineni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deepali","family":"Rawat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9887-6753","authenticated-orcid":true,"given":"Pankaj","family":"Suman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,10,11]]},"reference":[{"key":"pntd.0009841.ref001","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1186\/s40409-017-0127-6","article-title":"Snakebite envenomation turns again into a neglected tropical disease!","volume":"23","author":"JP Chippaux","year":"2017","journal-title":"The journal of venomous animals and toxins including tropical diseases"},{"key":"pntd.0009841.ref002","doi-asserted-by":"crossref","first-page":"17063","DOI":"10.1038\/nrdp.2017.63","article-title":"Snakebite envenoming","volume":"3","author":"J Guti\u00e9rrez","year":"2017","journal-title":"Nat Rev Dis Primers"},{"issue":"12","key":"pntd.0009841.ref003","doi-asserted-by":"crossref","first-page":"e0007899","DOI":"10.1371\/journal.pntd.0007899","article-title":"Beyond the \u2018big four\u2019: Venom profiling of the medically important yet neglected Indian snakes reveals disturbing antivenom deficiencies","volume":"13","author":"RR Senji Laxme","year":"2019","journal-title":"PLoS Negl Trop Dis"},{"issue":"1","key":"pntd.0009841.ref004","first-page":"10","article-title":"Green medicine as a harmonizing tool to antivenom therapy for the clinical management of snakebite: the road ahead","volume":"136","author":"AK Mukherjee","year":"2012","journal-title":"The Indian journal of medical research"},{"key":"pntd.0009841.ref005","unstructured":"Geneva. 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