{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T20:47:15Z","timestamp":1790801235455,"version":"4.1.0"},"update-to":[{"DOI":"10.1371\/journal.pntd.0009985","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2021,12,31]],"date-time":"2021-12-31T00:00:00Z","timestamp":1640908800000}}],"reference-count":42,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2021,12,17]],"date-time":"2021-12-17T00:00:00Z","timestamp":1639699200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100017923","name":"parafrap","doi-asserted-by":"crossref","award":["ANR-11-LABX-0024-PARAFRAP"],"award-info":[{"award-number":["ANR-11-LABX-0024-PARAFRAP"]}],"id":[{"id":"10.13039\/100017923","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100006251","name":"Universit\u00e9 de Bordeaux","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006251","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004794","name":"Centre National de la Recherche Scientifique","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004794","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007204","name":"Centre de Coop\u00e9ration Internationale en Recherche Agronomique pour le D\u00e9veloppement","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007204","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Centre international de Recherche-D\u00e9veloppement sur l'Elevage en zone Subhumide"}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>African trypanosomosis, a parasitic disease caused by protozoan parasites transmitted by tsetse flies, affects both humans and animals in sub-Saharan Africa. While the human form (HAT) is now limited to foci, the animal form (AAT) is widespread and affects the majority of sub-Saharan African countries, and constitutes a real obstacle to the development of animal breeding. The control of AAT is hampered by a lack of standardized and easy-to used diagnosis tools. This study aimed to evaluate the diagnostic potential of TbLysoPLA and TbGK proteins from <jats:italic>Trypanosoma brucei brucei<\/jats:italic> for AAT serodiagnosis in indirect ELISA using experimental and field sera, individually, in combination, and associated with the BiP C-terminal domain (C25) from <jats:italic>T<\/jats:italic>. <jats:italic>congolense<\/jats:italic>. These novel proteins were characterized <jats:italic>in silico<\/jats:italic>, and their sequence analysis showed strong identities with their orthologs in other trypanosomes (more than 60% for TbLysoPLA and more than 82% for TbGK). TbLysoPLA displays a low homology with cattle (&lt;35%) and <jats:italic>Piroplasma<\/jats:italic> (&lt;15%). However, TbGK shares more than 58% with cattle and between 45\u201355% with <jats:italic>Piroplasma<\/jats:italic>. We could identify seven predicted epitopes on TbLysoPLA sequence and 14 potential epitopes on TbGK. Both proteins were recombinantly expressed in <jats:italic>Escherichia coli<\/jats:italic>. Their diagnostic potential was evaluated by ELISA with sera from cattle experimentally infected with <jats:italic>T<\/jats:italic>. <jats:italic>congolense<\/jats:italic> and with <jats:italic>T<\/jats:italic>.<jats:italic>b<\/jats:italic>. <jats:italic>brucei<\/jats:italic>, sera from cattle naturally infected with <jats:italic>T<\/jats:italic>. <jats:italic>congolense<\/jats:italic>, <jats:italic>T<\/jats:italic>. <jats:italic>vivax<\/jats:italic> and <jats:italic>T<\/jats:italic>.<jats:italic>b<\/jats:italic>. <jats:italic>brucei<\/jats:italic>. Both proteins used separately had poor diagnostic performance. However, used together with the BiP protein, they showed 60% of sensitivity and between 87\u201396% of specificity, comparable to reference ELISA tests. In conclusion, we showed that the performance of the protein combinations is much better than the proteins tested individually for the diagnosis of AAT.<\/jats:p>","DOI":"10.1371\/journal.pntd.0009985","type":"journal-article","created":{"date-parts":[[2021,12,17]],"date-time":"2021-12-17T19:06:58Z","timestamp":1639768018000},"page":"e0009985","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":9,"title":["Novel protein candidates for serodiagnosis of African animal trypanosomosis: Evaluation of the diagnostic potential of lysophospholipase and glycerol kinase from Trypanosoma brucei"],"prefix":"10.1371","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4218-3269","authenticated-orcid":true,"given":"Magamba","family":"Tounkara","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain","family":"Boulang\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magali","family":"Thonnus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fr\u00e9d\u00e9ric","family":"Bringaud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7405-1626","authenticated-orcid":true,"given":"Adrien Marie Gaston","family":"B\u00e9lem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zakaria","family":"Bengaly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6059-5884","authenticated-orcid":true,"given":"Sophie","family":"Th\u00e9venon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3283-6588","authenticated-orcid":true,"given":"David","family":"Berthier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lo\u00efc","family":"Rivi\u00e8re","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,12,17]]},"reference":[{"key":"pntd.0009985.ref001","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2013\/194176","article-title":"Trypanosoma evansi and Surra: A Review and Perspectives on Origin, History, Distribution, Taxonomy, Morphology, Hosts, and Pathogenic Effects","volume":"2013","author":"M Desquesnes","year":"2013","journal-title":"BioMed Research International"},{"key":"pntd.0009985.ref002","first-page":"331","volume-title":"The TrypanosomiasesCAB International, Wallingford, UK. 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