{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T05:10:05Z","timestamp":1783055405206,"version":"3.54.6"},"reference-count":65,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2022,1,11]],"date-time":"2022-01-11T00:00:00Z","timestamp":1641859200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NHRI, Taiwan","award":["grant no. NHRI-MR-110-GP-12"],"award-info":[{"award-number":["grant no. NHRI-MR-110-GP-12"]}]},{"name":"NHRI,Taiwan","award":["grant no. NHRI-MR-110-CO-03"],"award-info":[{"award-number":["grant no. NHRI-MR-110-CO-03"]}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>Dengue fever is one of the most severe viral diseases transmitted by <jats:italic>Aedes<\/jats:italic> mosquitoes, with traditional approaches of disease control proving insufficient to prevent significant disease burden. Release of <jats:italic>Wolbachia<\/jats:italic>-transinfected mosquitoes offers a promising alternative control methodologies; <jats:italic>Wolbachia<\/jats:italic>-transinfected female <jats:italic>Aedes aegypti<\/jats:italic> demonstrate reduced dengue virus transmission, whilst <jats:italic>Wolbachia<\/jats:italic>-transinfected males cause zygotic lethality when crossed with uninfected females, providing a method for suppressing mosquito populations. Although highly promising, the delicate nature of population control strategies and differences between local species populations means that controlled releases of <jats:italic>Wolbachia<\/jats:italic>-transinfected mosquitoes cannot be performed without extensive testing on specific local <jats:italic>Ae<\/jats:italic>. <jats:italic>aegypti<\/jats:italic> populations. In order to investigate the potential for using <jats:italic>Wolbachia<\/jats:italic> to suppress local <jats:italic>Ae<\/jats:italic>. <jats:italic>aegypti<\/jats:italic> populations in Taiwan, we performed lab-based and semi-field fitness trials. We first transinfected the <jats:italic>Wolbachia<\/jats:italic> strain <jats:italic>w<\/jats:italic>AlbB into a local <jats:italic>Ae<\/jats:italic>. <jats:italic>aegypti<\/jats:italic> population (<jats:italic>w<\/jats:italic>AlbB-Tw) and found no significant changes in lifespan, fecundity and fertility when compared to controls. In the laboratory, we found that as the proportion of released male mosquitoes carrying <jats:italic>Wolbachia<\/jats:italic> was increased, population suppression could reach up to 100%. Equivalent experiments in semi-field experiments found suppression rates of up to 70%. The release of different ratios of wAlbB-Tw males in the semi-field system provided an estimate of the optimal size of male releases. Our results indicate that <jats:italic>w<\/jats:italic>AlbB-Tw has significant potential for use in vector control strategies aimed at <jats:italic>Ae<\/jats:italic>. <jats:italic>aegypti<\/jats:italic> population suppression in Taiwan. Open field release trials are now necessary to confirm that <jats:italic>w<\/jats:italic>AlbB-Tw mediated suppression is feasible in natural environments.<\/jats:p>","DOI":"10.1371\/journal.pntd.0010084","type":"journal-article","created":{"date-parts":[[2022,1,11]],"date-time":"2022-01-11T18:34:32Z","timestamp":1641926072000},"page":"e0010084","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":25,"title":["Lab-scale characterization and semi-field trials of Wolbachia Strain wAlbB in a Taiwan Wolbachia introgressed Ae. aegypti strain"],"prefix":"10.1371","volume":"16","author":[{"given":"Wei-Liang","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5942-3444","authenticated-orcid":true,"given":"Hui-Ying","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Xuan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo-Yu","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiang Ning","family":"Leaw","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5444-3999","authenticated-orcid":true,"given":"Cheng-Han","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6186-211X","authenticated-orcid":true,"given":"Matthew-P.","family":"Su","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling-Shan","family":"Tsai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7749-7807","authenticated-orcid":true,"given":"Shin-Hong","family":"Shiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7786-012X","authenticated-orcid":true,"given":"Zhiyong","family":"Xi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna C-C","family":"Jang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6352-1370","authenticated-orcid":true,"given":"Chun-Hong","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,1,11]]},"reference":[{"key":"pntd.0010084.ref001","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1038\/nature12060","article-title":"The global distribution and burden of dengue","volume":"496","author":"S Bhatt","year":"2013","journal-title":"Nature"},{"key":"pntd.0010084.ref002","volume-title":"Comprehensive guidelines for prevention and control of dengue and dengue haemorrhagic fever","author":"World Health Organization. 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