{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T03:16:08Z","timestamp":1791515768807,"version":"4.3.4"},"update-to":[{"DOI":"10.1371\/journal.pntd.0010139","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2022,4,25]],"date-time":"2022-04-25T00:00:00Z","timestamp":1650844800000}}],"reference-count":70,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T00:00:00Z","timestamp":1649808000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"crossref","award":["1132412 and 1118640"],"award-info":[{"award-number":["1132412 and 1118640"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>\n                    The arbovirus vector\n                    <jats:italic>Aedes albopictus<\/jats:italic>\n                    (Asian tiger mosquito) is common throughout the Indo-Pacific region, where most global dengue transmission occurs. We analysed population genomic data and tested for cryptic species in 160\n                    <jats:italic>Ae<\/jats:italic>\n                    .\n                    <jats:italic>albopictus<\/jats:italic>\n                    sampled from 16 locations across this region. We found no evidence of cryptic\n                    <jats:italic>Ae<\/jats:italic>\n                    .\n                    <jats:italic>albopictus<\/jats:italic>\n                    but found multiple intraspecific COI haplotypes partitioned into groups representing three Asian lineages: East Asia, Southeast Asia and Indonesia. Papua New Guinea (PNG), Vanuatu and Christmas Island shared recent coancestry, and Indonesia and Timor-Leste were likely invaded from East Asia. We used a machine learning trained on morphologically sexed samples to classify sexes using multiple genetic features and then characterized the\n                    <jats:italic>w<\/jats:italic>\n                    AlbA and\n                    <jats:italic>w<\/jats:italic>\n                    AlbB\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    infections in 664 other samples. The\n                    <jats:italic>w<\/jats:italic>\n                    AlbA and\n                    <jats:italic>w<\/jats:italic>\n                    AlbB infections as detected by qPCR showed markedly different patterns in the sexes. For females, most populations had a very high double infection incidence, with 67% being the lowest value (from Timor-Leste). For males, the incidence of double infections ranged from 100% (PNG) to 0% (Vanuatu). Only 6 females were infected solely by the\n                    <jats:italic>w<\/jats:italic>\n                    AlbA infection, while rare uninfected mosquitoes were found in both sexes. The\n                    <jats:italic>w<\/jats:italic>\n                    AlbA and\n                    <jats:italic>w<\/jats:italic>\n                    AlbB densities varied significantly among populations. For mosquitoes from Torres Strait and Vietnam, the\n                    <jats:italic>w<\/jats:italic>\n                    AlbB density was similar in single-infected and superinfected (\n                    <jats:italic>w<\/jats:italic>\n                    AlbA and\n                    <jats:italic>w<\/jats:italic>\n                    AlbB) mosquitoes. There was a positive association between\n                    <jats:italic>w<\/jats:italic>\n                    AlbA and\n                    <jats:italic>w<\/jats:italic>\n                    AlbB infection densities in superinfected\n                    <jats:italic>Ae<\/jats:italic>\n                    .\n                    <jats:italic>albopictus<\/jats:italic>\n                    . Our findings provide no evidence of cryptic species of\n                    <jats:italic>Ae<\/jats:italic>\n                    .\n                    <jats:italic>albopictus<\/jats:italic>\n                    in the region and suggest site-specific factors influencing the incidence of\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    infections and their densities. We also demonstrate the usefulness of ddRAD tag depths as sex-specific mosquito markers. The results provide baseline data for the exploitation of\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    -induced cytoplasmic incompatibility (CI) in dengue control.\n                  <\/jats:p>","DOI":"10.1371\/journal.pntd.0010139","type":"journal-article","created":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T13:54:13Z","timestamp":1649858053000},"page":"e0010139","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":15,"title":["Sex-specific distribution and classification of Wolbachia infections and mitochondrial DNA haplogroups in Aedes albopictus from the 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