{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T16:45:22Z","timestamp":1789231522506,"version":"build-2803163510"},"update-to":[{"DOI":"10.1371\/journal.pntd.0010171","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2022,2,3]],"date-time":"2022-02-03T00:00:00Z","timestamp":1643846400000}}],"reference-count":28,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2022,1,24]],"date-time":"2022-01-24T00:00:00Z","timestamp":1642982400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007843","name":"Norwegian Agency for Development Cooperation","doi-asserted-by":"crossref","award":["RAF-3058 KEN-18\/0005"],"award-info":[{"award-number":["RAF-3058 KEN-18\/0005"]}],"id":[{"id":"10.13039\/100007843","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100007843","name":"Norad","doi-asserted-by":"crossref","award":["CAP-Africa project"],"award-info":[{"award-number":["CAP-Africa project"]}],"id":[{"id":"10.13039\/100007843","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Wellcome Trust International Intermediate Fellowship","award":["222005\/Z\/20\/Z"],"award-info":[{"award-number":["222005\/Z\/20\/Z"]}]},{"DOI":"10.13039\/100009131","name":"Swiss Agency for Development and Cooperation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100009131","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100004441","name":"Swedish International Development Cooperation Agency","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100004441","id-type":"DOI","asserted-by":"crossref"}]},{"name":"UK Aid, Government of the United Kingdom"},{"name":"Ministry of Higher Education, Science and Technology, Kenya"},{"name":"Government of the Federal Democratic Republic of Ethiopia"}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p><jats:italic>Aedes simpsoni<\/jats:italic> complex has a wide distribution in Africa and comprises at least three described sub-species including the yellow fever virus (YFV) vector <jats:italic>Ae<\/jats:italic>. <jats:italic>bromeliae<\/jats:italic>. To date, the distribution and relative contributions of the sub-species and\/or subpopulations including bionomic characteristics in relation to YF transmission dynamics remain poorly studied. In this study conducted in two areas with divergent ecosystems: peri-urban (coastal Rabai) and rural (Rift Valley Kerio Valley) in Kenya, survival rate was estimated by parity in <jats:italic>Ae<\/jats:italic>. <jats:italic>simpsoni s<\/jats:italic>.<jats:italic>l<\/jats:italic>. mosquitoes sampled using CO<jats:sub>2<\/jats:sub>-baited BG Sentinel traps. We then applied PCR targeting the nuclear internal transcribed spacer 2 (ITS2), region followed by sequencing and phylogenetic analytics to identify the sibling species in the <jats:italic>Ae<\/jats:italic>. <jats:italic>simpsoni<\/jats:italic> complex among parous and blood fed cohorts. Our results show that <jats:italic>Ae<\/jats:italic>. <jats:italic>bromeliae<\/jats:italic> was the most dominant sub-species in both areas, exhibiting high survival rates, human blood-feeding, and potentially, high vectorial capacity for pathogen transmission. We document for the first time the presence of <jats:italic>Ae<\/jats:italic>. <jats:italic>lilii<\/jats:italic> in Kenya and potentially yet-to-be described species in the complex displaying human feeding tendencies. We also infer a wide host feeding range on rodents, reptile, and domestic livestock besides humans especially for <jats:italic>Ae<\/jats:italic>. <jats:italic>bromeliae<\/jats:italic>. This feeding trend could likely expose humans to various zoonotic pathogens. Taken together, we highlight the utility of genotype-based analyses to generate precision surveillance data of vector populations for enhanced disease risk prediction and to guide cost-effective interventions (e.g. YF vaccinations).<\/jats:p>","DOI":"10.1371\/journal.pntd.0010171","type":"journal-article","created":{"date-parts":[[2022,1,24]],"date-time":"2022-01-24T18:37:28Z","timestamp":1643049448000},"page":"e0010171","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":15,"title":["Survival rate, blood feeding habits and sibling species composition of Aedes simpsoni complex: Implications for arbovirus transmission risk in East Africa"],"prefix":"10.1371","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8890-6277","authenticated-orcid":true,"given":"Winnie W.","family":"Kamau","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosemary","family":"Sang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4415-8366","authenticated-orcid":true,"given":"Edwin O.","family":"Ogola","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilbert","family":"Rotich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5990-9492","authenticated-orcid":true,"given":"Caroline","family":"Getugi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5480-6865","authenticated-orcid":true,"given":"Sheila B.","family":"Agha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nelson","family":"Menza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5080-9903","authenticated-orcid":true,"given":"Baldwyn","family":"Torto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9910-4704","authenticated-orcid":true,"given":"David P.","family":"Tchouassi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,1,24]]},"reference":[{"key":"pntd.0010171.ref001","unstructured":"WHO Disease Outbreak News (DONs). https:\/\/www.who.int\/emergencies\/disease-outbreak-news\/4."},{"key":"pntd.0010171.ref002","unstructured":"WHO Regional Office for Africa. Yellow fever. https:\/\/www.afro.who.int\/health-topics\/yellow-fever."},{"issue":"5","key":"pntd.0010171.ref003","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1002\/rmv.584","article-title":"The enigma of yellow fever in East Africa.","volume":"18","author":"BR Ellis","year":"2008","journal-title":"Rev Med Virol"},{"issue":"4","key":"pntd.0010171.ref004","doi-asserted-by":"crossref","first-page":"650","DOI":"10.4269\/ajtmh.1998.59.650","article-title":"First recorded outbreak of Yellow fever in Kenya, 1992\u201393 II. 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