{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T10:57:53Z","timestamp":1790506673405,"version":"4.1.0"},"update-to":[{"DOI":"10.1371\/journal.pntd.0010175","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2022,2,10]],"date-time":"2022-02-10T00:00:00Z","timestamp":1644451200000}}],"reference-count":46,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2022,1,31]],"date-time":"2022-01-31T00:00:00Z","timestamp":1643587200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81860361"],"award-info":[{"award-number":["81860361"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82060371"],"award-info":[{"award-number":["82060371"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32060223"],"award-info":[{"award-number":["32060223"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Xinjiang Uyghur Autonomous Region Key Laboratory Open Research Program Project","award":["2020D04028"],"award-info":[{"award-number":["2020D04028"]}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:sec id=\"sec001\"><jats:title>Background<\/jats:title><jats:p>Alveolar echinococcosis (AE) can cause severe liver fibrosis and could be fatal if left untreated. Currently, there are no effective therapeutic options for AE-induced liver fibrosis. In view of the therapeutic potential of adipose-derived stem cells (ADSCs), we investigated whether ADSCs transplantation has the ability to control or reverse fibrosis progression in the liver of<jats:italic>Echinococcus multilocularis<\/jats:italic>(<jats:italic>E<\/jats:italic>.<jats:italic>multilocularis<\/jats:italic>) infected mice.<\/jats:p><\/jats:sec><jats:sec id=\"sec002\"><jats:title>Methodology\/Principal findings<\/jats:title><jats:p>C57BL\/6 mice infected with<jats:italic>E<\/jats:italic>.<jats:italic>multilocularis<\/jats:italic>through portal vein inoculation were intravenously injected with ADSCs isolated from inguinal adipose tissues of 6\u20138 weeks old mice. Histopathological analysis including heamatoxylin &amp; eosin staining as well as Masson\u2019s trichrome staining, and Sirius red staining were performed to access the degree of liver fibrosis. Histopathological examination 30 days after ADSCs transplantation revealed that ADSCs significantly decreased the degree of liver fibrosis in<jats:italic>E<\/jats:italic>.<jats:italic>multilocularis<\/jats:italic>infected mice by inhibiting the expressions of \u03b1-SMA and type 1 collagen deposition. In addition, compared to the non-transplanted group, ADSCs transplantation reduced fibrotic areas in<jats:italic>E<\/jats:italic>.<jats:italic>multilocularis<\/jats:italic>infected mice. We also found that ADSCs transplantation significantly down-regulated TGF-\u03b21 and TGF-\u03b2R expressions, while up-regulating Smad7 expression in the TGF-\u03b2\/Smad signaling pathway.<\/jats:p><\/jats:sec><jats:sec id=\"sec003\"><jats:title>Conclusions<\/jats:title><jats:p>ADSCs can alleviate<jats:italic>Echinococcus multilocularis<\/jats:italic>infection-induced liver fibrosis by modulating the activity level of the TGF-\u03b2\/Smad7 signaling pathway and provide a potential therapeutic approach for<jats:italic>E<\/jats:italic>.<jats:italic>multilocularis<\/jats:italic>-induced fibrosis.<\/jats:p><\/jats:sec>","DOI":"10.1371\/journal.pntd.0010175","type":"journal-article","created":{"date-parts":[[2022,1,31]],"date-time":"2022-01-31T18:35:59Z","timestamp":1643654159000},"page":"e0010175","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":27,"title":["Transplantation of adipose-derived stem cells ameliorates Echinococcus multilocularis-induced liver fibrosis in mice"],"prefix":"10.1371","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7457-6307","authenticated-orcid":true,"given":"Ning","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenmei","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1207-4480","authenticated-orcid":true,"given":"Ying","family":"Ke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Chu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guodong","family":"L\u00fc","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojuan","family":"Bi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6210-4719","authenticated-orcid":true,"given":"Renyong","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,1,31]]},"reference":[{"issue":"5","key":"pntd.0010175.ref001","doi-asserted-by":"crossref","first-page":"e0007640","DOI":"10.1371\/journal.pntd.0007640","article-title":"Regulation of hepatic microRNAs in response to early stage Echinococcus multilocularis egg infection in C57BL\/6 mice.","volume":"14","author":"G Boubaker","year":"2020","journal-title":"PLoS Negl Trop Dis"},{"issue":"10","key":"pntd.0010175.ref002","doi-asserted-by":"crossref","first-page":"e1877","DOI":"10.1371\/journal.pntd.0001877","article-title":"Sensitive and specific immunohistochemical diagnosis of human alveolar echinococcosis with the monoclonal antibody Em2G11","volume":"6","author":"TF Barth","year":"2012","journal-title":"PLoS Negl Trop Dis"},{"issue":"1","key":"pntd.0010175.ref003","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.jhep.2008.03.023","article-title":"Alveolar echinococcosis: from a deadly disease to a well-controlled infection. 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