{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:52:09Z","timestamp":1792788729977,"version":"4.5.0"},"update-to":[{"DOI":"10.1371\/journal.pntd.0010249","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2022,7,27]],"date-time":"2022-07-27T00:00:00Z","timestamp":1658880000000}}],"reference-count":116,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2022,7,15]],"date-time":"2022-07-15T00:00:00Z","timestamp":1657843200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Cooperative and Cultural Action Service","award":["959139G"],"award-info":[{"award-number":["959139G"]}]},{"name":"Strasbourg Institute of Parasitology and Tropical Diseases"}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Undernutrition and schistosomiasis are public health problems and often occur in low and middle-income countries. Protein undernutrition can alter the host-parasite environment system and aggravate the course of schistosomiasis. This study aimed to assess the impact of a low-protein diet on the efficacy of praziquantel.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methodology\/Principal findings<\/jats:title>\n                    <jats:p>\n                      Thirty-day-old mice were fed with a low-protein diet, and 40 days later, they were individually infected with fifty\n                      <jats:italic>Schistosoma mansoni<\/jats:italic>\n                      cercariae. A 28-day-treatment with praziquantel at 100 mg\/kg for five consecutive days followed by distilled water begins on the 36\n                      <jats:sup>th<\/jats:sup>\n                      day post-infection. Mice were sacrificed on the 64\n                      <jats:sup>th<\/jats:sup>\n                      day post-infection. We determined the parasitological burden, liver and intestine histomorphometry, liver injury, and immunomodulation parameters. Praziquantel treatment of infected mice fed with a standard diet (IN-PZQ) resulted in a significant reduction of worm and egg burdens and a normalization of iron and calcium levels. The therapy also improved schistosomiasis-induced hepatopathy and oxidative stress. The anti-inflammatory and immunomodulatory activities of praziquantel were also significant in these mice. When infected mice receiving the low-protein diet were treated with praziquantel (ILP-PZQ), the body weight loss and hepatomegaly were not alleviated, and the worm and liver egg burdens were significantly higher than those of IN-PZQ mice (\n                      <jats:italic>P<\/jats:italic>\n                      &lt; 0.001). The treatment did not reduce the increased activities of ALT and \u03b3-GGT, the high malondialdehyde concentration, and the liver granuloma volume. The iron and calcium levels were not ameliorated and differed from those of IN-PZQ mice (\n                      <jats:italic>P<\/jats:italic>\n                      &lt; 0.001 and\n                      <jats:italic>P<\/jats:italic>\n                      &lt; 0.05). Moreover, in these mice, praziquantel treatment did not reverse the high level of IL-5 and the low mRNA expression of CCL3\/MIP-1\u03b1 and CXCL-10\/IP-10 induced by\n                      <jats:italic>S<\/jats:italic>\n                      .\n                      <jats:italic>mansoni<\/jats:italic>\n                      infection.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Conclusion\/Significance<\/jats:title>\n                    <jats:p>These results demonstrated that a low-protein diet reduced the schistosomicidal, antioxidant, anti-inflammatory, and immunomodulatory activities of praziquantel.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pntd.0010249","type":"journal-article","created":{"date-parts":[[2022,7,15]],"date-time":"2022-07-15T13:36:12Z","timestamp":1657892172000},"page":"e0010249","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":9,"title":["Protein undernutrition reduces the efficacy of praziquantel in a murine model of Schistosoma mansoni infection"],"prefix":"10.1371","volume":"16","author":[{"given":"Joseph Bertin","family":"Kadji Fassi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9838-8570","authenticated-orcid":true,"given":"Hermine","family":"Boukeng Jatsa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrich","family":"Membe Femoe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentin","family":"Greigert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Brunet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Cannet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian M\u00e9rim\u00e9","family":"Kenfack","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nestor","family":"Gipwe Feussom","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilienne","family":"Tienga Nkondo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed","family":"Abou-Bacar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander Wilhelm","family":"Pfaff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren\u00e9","family":"Kamgang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Kamtchouing","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louis-Albert","family":"Tchuem Tchuent\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,7,15]]},"reference":[{"key":"pntd.0010249.ref001","unstructured":"WHO. 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