{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T19:10:53Z","timestamp":1791573053198,"version":"4.3.4"},"update-to":[{"DOI":"10.1371\/journal.pntd.0010251","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2022,3,24]],"date-time":"2022-03-24T00:00:00Z","timestamp":1648080000000}}],"reference-count":43,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2022,3,14]],"date-time":"2022-03-14T00:00:00Z","timestamp":1647216000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Merck Sharp and Dohme Animal Health GmbH"},{"name":"Bahia State Research Support Foundation (FAPESB) and the State University of Santa Cruz"},{"DOI":"10.13039\/501100020648","name":"State University of Santa Cruz","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100020648","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Coordination for the Improvement of Higher Education Personnel - Brazil","award":["Finance Code 001"],"award-info":[{"award-number":["Finance Code 001"]}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>The sand flea <jats:italic>Tunga penetrans<\/jats:italic> is one of the zoonotic agents of tungiasis, a parasitic skin disease of humans and animals. The dog is one of its main reservoirs. This negatively controlled, randomized, double-masked clinical trial evaluated the therapeutic and residual efficacy of fluralaner for treatment of dogs naturally infested with <jats:italic>T<\/jats:italic>. <jats:italic>penetrans<\/jats:italic>. Sixty-two dogs from an endemically affected community in Brazil were randomly assigned to either receive oral fluralaner (Bravecto chewable tablets) at a dose of 25 to 56 mg fluralaner\/kg body weight, or no treatment (31 dogs per group). Dogs were clinically examined using a severity score for acute canine tungiasis (SCADT), parasitological examinations as defined by the Fortaleza classification, and pictures of lesions on days 0 (inclusion and treatment), 7 \u00b1 2, 14 \u00b1 2, 21 \u00b1 2, 28 \u00b1 2, 60 \u00b1 7, 90 \u00b1 7, 120 \u00b1 7 and 150 \u00b1 7. The percentage of parasite-free dogs after treatment was &gt;90% between days 14 and 90 post-treatment with 100% efficacy on study days 21, 28 and 60. Sand flea counts on fluralaner treated dogs were significantly lower (p&lt;0.025) than control dogs on all counts from day 7 to 120. The number of live sand fleas on treated dogs was reduced by &gt; 90% on day 7, &gt; 95% on days 14 and 90, and 100% from day 21 to 60, and with a significant difference between groups from day 7 to 120. From day 7 to day 120, mean SCADT scores were significantly reduced in treated dogs with a mean of 0.10 compared to 1.54 on day 120 in untreated dogs. Therefore, a single oral fluralaner administration is effective for treating and achieving long lasting (&gt; 12 weeks) prevention for tungiasis in dogs.<\/jats:p>","DOI":"10.1371\/journal.pntd.0010251","type":"journal-article","created":{"date-parts":[[2022,3,14]],"date-time":"2022-03-14T17:53:57Z","timestamp":1647280437000},"page":"e0010251","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":11,"title":["Efficacy of oral fluralaner (Bravecto) against Tunga penetrans in dogs: A negative control, randomized field study in an endemic community in Brazil"],"prefix":"10.1371","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8940-2548","authenticated-orcid":true,"given":"Katharine Costa","family":"dos Santos","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael Marin","family":"Chiummo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4098-6989","authenticated-orcid":true,"given":"Anja Regina","family":"Heckeroth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Zschiesche","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2961-8069","authenticated-orcid":true,"given":"Paula Elisa","family":"Brand\u00e3o Guedes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8118-7713","authenticated-orcid":true,"given":"Tatiani Vitor","family":"Harvey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6260-0541","authenticated-orcid":true,"given":"Anderson Vieira","family":"de Jesus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anai\u00e1","family":"da Paix\u00e3o Sev\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0353-0731","authenticated-orcid":true,"given":"Joana Thaisa Santos","family":"de Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zelina","family":"dos Santos Freire","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00fcrgen","family":"Kr\u00fccken","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8888-6269","authenticated-orcid":true,"given":"Fernando","family":"de Almeida Borges","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6043-2038","authenticated-orcid":true,"given":"Georg","family":"von Samson-Himmelstjerna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renata Santiago","family":"Alberto Carlos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,3,14]]},"reference":[{"issue":"1","key":"pntd.0010251.ref001","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1586\/14787210.4.1.151","article-title":"Revision on tungiasis: Treatment options and prevention","volume":"4","author":"J. 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