{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T18:14:36Z","timestamp":1776708876773,"version":"3.51.2"},"update-to":[{"DOI":"10.1371\/journal.pntd.0010284","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2022,5,2]],"date-time":"2022-05-02T00:00:00Z","timestamp":1651449600000}}],"reference-count":21,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2022,4,20]],"date-time":"2022-04-20T00:00:00Z","timestamp":1650412800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Tahija Foundation"},{"name":"Tahija Foundation"},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["GNT1042782"],"award-info":[{"award-number":["GNT1042782"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>The Applying <jats:italic>Wolbachia<\/jats:italic> to Eliminate Dengue (AWED) trial was a parallel cluster randomised trial that demonstrated <jats:italic>Wolbachia<\/jats:italic> (<jats:italic>w<\/jats:italic>Mel) introgression into <jats:italic>Ae<\/jats:italic>. <jats:italic>aegypti<\/jats:italic> populations reduced dengue incidence. In this predefined substudy, we compared between treatment arms, the relative abundance of <jats:italic>Ae<\/jats:italic>. <jats:italic>aegypti<\/jats:italic> and <jats:italic>Ae<\/jats:italic>. <jats:italic>albopictus<\/jats:italic> before, during and after <jats:italic>w<\/jats:italic>Mel-introgression. Between March 2015 and March 2020, 60,084 BG trap collections yielded 478,254 <jats:italic>Ae<\/jats:italic>. <jats:italic>aegypti<\/jats:italic> and 17,623 <jats:italic>Ae<\/jats:italic>. <jats:italic>albopictus<\/jats:italic>. Between treatment arms there was no measurable difference in <jats:italic>Ae<\/jats:italic>. <jats:italic>aegypti<\/jats:italic> relative abundance before or after <jats:italic>w<\/jats:italic>Mel-deployments, with a count ratio of 0.96 (95% CI 0.76, 1.21) and 1.00 (95% CI 0.85, 1.17) respectively. More <jats:italic>Ae<\/jats:italic>. <jats:italic>aegypti<\/jats:italic> were caught per trap per week in the <jats:italic>w<\/jats:italic>Mel-intervention arm compared to the control arm during <jats:italic>w<\/jats:italic>Mel deployments (count ratio 1.23 (95% CI 1.03, 1.46)). Between treatment arms there was no measurable difference in the <jats:italic>Ae<\/jats:italic>. <jats:italic>albopictus<\/jats:italic> population size before, during or after <jats:italic>w<\/jats:italic>Mel-deployment (overall count ratio 1.10 (95% CI 0.89, 1.35)). We also compared insecticide resistance phenotypes of <jats:italic>Ae<\/jats:italic>. <jats:italic>aegypti<\/jats:italic> in the first and second years after <jats:italic>w<\/jats:italic>Mel-deployments. <jats:italic>Ae<\/jats:italic>. <jats:italic>aegypti<\/jats:italic> field populations from <jats:italic>w<\/jats:italic>Mel-treated and untreated arms were similarly resistant to malathion (0.8%), permethrin (1.25%) and cyfluthrin (0.15%) in year 1 and year 2 of the trial. In summary, we found no between-arm differences in the relative abundance of <jats:italic>Ae<\/jats:italic>. <jats:italic>aegypti<\/jats:italic> or <jats:italic>Ae<\/jats:italic>. <jats:italic>albopictus<\/jats:italic> prior to or after <jats:italic>w<\/jats:italic>Mel introgression, and no between-arm difference in <jats:italic>Ae<\/jats:italic>. <jats:italic>aegypti<\/jats:italic> insecticide resistance phenotypes. These data suggest neither <jats:italic>Aedes<\/jats:italic> abundance, nor insecticide resistance, confounded the epidemiological outcomes of the AWED trial.<\/jats:p>","DOI":"10.1371\/journal.pntd.0010284","type":"journal-article","created":{"date-parts":[[2022,4,20]],"date-time":"2022-04-20T17:37:06Z","timestamp":1650476226000},"page":"e0010284","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":17,"title":["Aedes aegypti abundance and insecticide resistance profiles in the Applying Wolbachia to Eliminate Dengue trial"],"prefix":"10.1371","volume":"16","author":[{"given":"Warsito","family":"Tantowijoyo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephanie K.","family":"Tanamas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Indah","family":"Nurhayati","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sigit","family":"Setyawan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nida","family":"Budiwati","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iva","family":"Fitriana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Inggrid","family":"Ernesia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dwi Satria","family":"Wardana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Endah","family":"Supriyati","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7462-7904","authenticated-orcid":true,"given":"Eggi","family":"Arguni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yeti","family":"Meitika","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Equatori","family":"Prabowo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8511-0748","authenticated-orcid":true,"given":"Bekti","family":"Andari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benjamin R.","family":"Green","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lauren","family":"Hodgson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edwige","family":"Ranc\u00e8s","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0674-4441","authenticated-orcid":true,"given":"Peter A.","family":"Ryan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott L.","family":"O\u2019Neill","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0485-5826","authenticated-orcid":true,"given":"Katherine L.","family":"Anders","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3517-613X","authenticated-orcid":true,"given":"M. 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