{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T18:23:57Z","timestamp":1792002237600,"version":"4.4.0"},"reference-count":57,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2022,3,23]],"date-time":"2022-03-23T00:00:00Z","timestamp":1647993600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"publisher","award":["2015CB150300"],"award-info":[{"award-number":["2015CB150300"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the Yunnan Expert Workstation","award":["202005AF150041"],"award-info":[{"award-number":["202005AF150041"]}]},{"name":"the Veterinary Public Health Innovation Team of Yunnan Province","award":["202105AE160014"],"award-info":[{"award-number":["202105AE160014"]}]},{"name":"the Fund for Shanxi \u201c1331 Project\u201d","award":["20211331-13"],"award-info":[{"award-number":["20211331-13"]}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>The tropical liver fluke<jats:italic>Fasciola gigantica<\/jats:italic>is a parasitic helminth that has been frequently reported to infect mammals, typically involving water buffaloes. In this study, we characterized the tissue transcriptional landscape of buffaloes following infection by<jats:italic>F<\/jats:italic>.<jats:italic>gigantica<\/jats:italic>. RNAs were isolated from hepatic lymph nodes (hLNs), peripheral blood lymphocytes (pBLs), and spleen at 3-, 42- and 70-days post-infection (dpi), and all samples were subjected to RNA sequencing analyses. At 3 dpi, 2603, 460, and 162 differentially expressed transcripts (DETs) were detected in hLNs, pBLs, and spleen, respectively. At 42 dpi, 322, 937, and 196 DETs were detected in hLNs, pBLs, and spleen, respectively. At 70 dpi, 376, 334, and 165 DETs were detected in hLNs, pBLs, and spleen, respectively. Functional enrichment analysis identified upregulated immune-related pathways in the infected tissues involved in innate and adaptive immune responses, especially in hLNs at 42 and 70 dpi, and pBLs at 3 and 42 dpi. The upregulated transcripts in spleen were not enriched in any immune-related pathway. Co-expression network analysis further identified transcriptional changes associated with immune response to<jats:italic>F<\/jats:italic>.<jats:italic>gigantica<\/jats:italic>infection. Receiver operating characteristic (ROC) curve analysis showed that 107 genes in hLNs, 32 genes in pBLs, and 36 genes in spleen correlated with<jats:italic>F<\/jats:italic>.<jats:italic>gigantica<\/jats:italic>load. These findings provide new insight into molecular mechanisms and signaling pathways associated with<jats:italic>F<\/jats:italic>.<jats:italic>gigantica<\/jats:italic>infection in buffaloes.<\/jats:p>","DOI":"10.1371\/journal.pntd.0010286","type":"journal-article","created":{"date-parts":[[2022,3,23]],"date-time":"2022-03-23T17:34:05Z","timestamp":1648056845000},"page":"e0010286","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":7,"title":["Transcriptomic landscape of hepatic lymph nodes, peripheral blood lymphocytes and spleen of swamp buffaloes infected with the tropical liver fluke Fasciola 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