{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T20:08:15Z","timestamp":1792440495680,"version":"4.5.0"},"reference-count":69,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2022,4,26]],"date-time":"2022-04-26T00:00:00Z","timestamp":1650931200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fondo Mixto Consejo Nacional de Ciencia y Tecnologia (CONACYT) (Mexico)\u2013Gobierno del Estado de Yucatan","award":["YUC-2017-03-01-556"],"award-info":[{"award-number":["YUC-2017-03-01-556"]}]},{"DOI":"10.13039\/100000200","name":"United States Agency for International Development","doi-asserted-by":"publisher","award":["AID-OAA-F-16-00082"],"award-info":[{"award-number":["AID-OAA-F-16-00082"]}],"id":[{"id":"10.13039\/100000200","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Catedras-CONACYT program"}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      The combination of\n                      <jats:italic>Wolbachia<\/jats:italic>\n                      -based incompatible insect technique (IIT) and radiation-based sterile insect technique (SIT) can be used for population suppression of\n                      <jats:italic>Aedes aegypti<\/jats:italic>\n                      . Our main objective was to evaluate whether open-field mass-releases of\n                      <jats:italic>w<\/jats:italic>\n                      AlbB-infected\n                      <jats:italic>Ae<\/jats:italic>\n                      .\n                      <jats:italic>aegypti<\/jats:italic>\n                      males, as part of an Integrated Vector Management (IVM) plan led by the Mexican Ministry of Health, could suppress natural populations of\n                      <jats:italic>Ae<\/jats:italic>\n                      .\n                      <jats:italic>aegypti<\/jats:italic>\n                      in urbanized settings in south Mexico.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methodology\/Principal findings<\/jats:title>\n                    <jats:p>\n                      We implemented a controlled before-and-after quasi-experimental study in two suburban localities of Yucatan (Mexico): San Pedro Chimay (SPC), which received IIT-SIT, and San Antonio Tahdzibich\u00e9n used as control. Release of\n                      <jats:italic>w<\/jats:italic>\n                      AlbB\n                      <jats:italic>Ae<\/jats:italic>\n                      .\n                      <jats:italic>aegypti<\/jats:italic>\n                      males at SPC extended for 6 months (July-December 2019), covering the period of higher\n                      <jats:italic>Ae<\/jats:italic>\n                      .\n                      <jats:italic>aegypti<\/jats:italic>\n                      abundance. Entomological indicators included egg hatching rates and outdoor\/indoor adult females collected at the release and control sites. Approximately 1,270,000 lab-produced\n                      <jats:italic>w<\/jats:italic>\n                      AlbB-infected\n                      <jats:italic>Ae<\/jats:italic>\n                      .\n                      <jats:italic>aegypti<\/jats:italic>\n                      males were released in the 50-ha treatment area (2,000\n                      <jats:italic>w<\/jats:italic>\n                      AlbB\n                      <jats:italic>Ae<\/jats:italic>\n                      .\n                      <jats:italic>aegypti<\/jats:italic>\n                      males per hectare twice a week in two different release days, totaling 200,000 male mosquitoes per week). The efficacy of IIT-SIT in suppressing indoor female\n                      <jats:italic>Ae<\/jats:italic>\n                      .\n                      <jats:italic>aegypti<\/jats:italic>\n                      density (quantified from a generalized linear mixed model showing a statistically significant reduction in treatment versus control areas) was 90.9% a month after initiation of the suppression phase, 47.7% two months after (when number of released males was reduced in 50% to match local abundance), 61.4% four months after (when initial number of released males was re-established), 88.4% five months after and 89.4% at six months after the initiation of the suppression phase. A proportional, but lower, reduction in outdoor female\n                      <jats:italic>Ae<\/jats:italic>\n                      .\n                      <jats:italic>aegypti<\/jats:italic>\n                      was also quantified (range, 50.0\u201375.2% suppression).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Conclusions\/Significance<\/jats:title>\n                    <jats:p>\n                      Our study, the first open-field pilot implementation of\n                      <jats:italic>Wolbachia<\/jats:italic>\n                      IIT-SIT in Mexico and Latin-America, confirms that inundative male releases can significantly reduce natural populations of\n                      <jats:italic>Ae<\/jats:italic>\n                      .\n                      <jats:italic>aegypti<\/jats:italic>\n                      . More importantly, we present successful pilot results of the integration of\n                      <jats:italic>Wolbachia<\/jats:italic>\n                      IIT-SIT within a IVM plan implemented by Ministry of Health personnel.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pntd.0010324","type":"journal-article","created":{"date-parts":[[2022,4,26]],"date-time":"2022-04-26T13:27:54Z","timestamp":1650979674000},"page":"e0010324","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":81,"title":["Pilot trial using mass field-releases of sterile males produced with the incompatible and sterile insect techniques as part of integrated Aedes aegypti control in Mexico"],"prefix":"10.1371","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9303-2877","authenticated-orcid":true,"given":"Abdiel","family":"Mart\u00edn-Park","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Azael","family":"Che-Mendoza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yamili","family":"Contreras-Perera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"P\u00e9rez-Carrillo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henry","family":"Puerta-Guardo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1936-3624","authenticated-orcid":true,"given":"Josu\u00e9","family":"Villegas-Chim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillermo","family":"Guillermo-May","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anuar","family":"Medina-Barreiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2408-5128","authenticated-orcid":true,"given":"Hugo","family":"Delf\u00edn-Gonz\u00e1lez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa","family":"M\u00e9ndez-Vales","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Santos","family":"V\u00e1zquez-Narvaez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Palacio-Vargas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6193-1242","authenticated-orcid":true,"given":"Fabi\u00e1n","family":"Correa-Morales","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2829-6945","authenticated-orcid":true,"given":"Guadalupe","family":"Ayora-Talavera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norma","family":"Pav\u00eda-Ruz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Fu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3960-1036","authenticated-orcid":true,"given":"Dongjing","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohua","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Eugenia","family":"Toledo-Roman\u00ed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7786-012X","authenticated-orcid":true,"given":"Zhiyong","family":"Xi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0710-270X","authenticated-orcid":true,"given":"Gonzalo","family":"V\u00e1zquez-Prokopec","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0565-1342","authenticated-orcid":true,"given":"Pablo","family":"Manrique-Saide","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,4,26]]},"reference":[{"key":"pntd.0010324.ref001","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1038\/s41586-019-1407-9","article-title":"Incompatible and sterile insect techniques combined eliminate mosquitoes","volume":"572","author":"X Zheng","year":"2019","journal-title":"Nature"},{"key":"pntd.0010324.ref002","doi-asserted-by":"crossref","first-page":"2177","DOI":"10.1056\/NEJMoa2030243","article-title":"Efficacy of Wolbachia-Infected Mosquito Deployments for the Control of Dengue","volume":"10","author":"A Utarini","year":"2021","journal-title":"N Engl J Med"},{"key":"pntd.0010324.ref003","doi-asserted-by":"crossref","unstructured":"The Project Wolbachia\u2013Singapore Consortium, Ng Lee Ching. Wolbachia-mediated sterility suppresses Aedes aegypti populations in the urban tropics. medRxiv [Internet]. 2021 Jun [cited 2021 Aug 12]. Available from: https:\/\/www.medrxiv.org\/content\/10.1101\/2021.06.16.21257922v1. https:\/\/doi.org\/10.1101\/2021.06.16.21257922.","DOI":"10.1101\/2021.06.16.21257922"},{"issue":"21","key":"pntd.0010324.ref004","first-page":"00164","article-title":"Wolbachia as translational science: controlling mosquito-borne pathogens","volume":"21S1471\u20134922","author":"EP Caragata","year":"2021","journal-title":"Trends Parasitol"},{"key":"pntd.0010324.ref005","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1038\/s41587-020-0471-x","article-title":"Efficient pro- duction of male Wolbachia-infected Aedes aegypti mosquitoes enables large-scale suppression of wild populations","volume":"38","author":"JE Crawford","year":"2020","journal-title":"Nat Biotechnol"},{"issue":"41","key":"pntd.0010324.ref006","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2106828118","article-title":"Releasing incompatible males drives strong suppression across populations of wild and Wolbachia carrying Aedes aegypti in Australia","volume":"118","author":"NW Beebe","year":"2021","journal-title":"PNAS"},{"key":"pntd.0010324.ref007","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1038\/nrmicro2968","article-title":"Beyond insecticides: new thinking on an ancient problem","volume":"11","author":"EA McGraw","year":"2013","journal-title":"Nat Rev Microbiol"},{"key":"pntd.0010324.ref008","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1038\/s41579-018-0025-0","article-title":"Controlling vector-borne diseases by releasing modified mosquitoes","volume":"16","author":"Flores HA","year":"2018","journal-title":"Nat Rev Microbiol"},{"key":"pntd.0010324.ref009","first-page":"tjab025","article-title":"When More is Less: Mosquito Population Suppression Using Sterile, Incompatible and Genetically Modified Male Mosquitoes","volume":"11","author":"SL Dobson","year":"2021","journal-title":"J Med Entomol"},{"key":"pntd.0010324.ref010","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1126\/science.1117607","article-title":"Wolbachia establishment and invasion in an Aedes aegypti laboratory population","volume":"310","author":"Z Xi","year":"2005","journal-title":"Sci"},{"key":"pntd.0010324.ref011","doi-asserted-by":"crossref","first-page":"28792","DOI":"10.1038\/srep28792","article-title":"The wMel strain of Wolbachia reduces transmission of Zika virus by Aedes aegypti","volume":"6","author":"MT Aliota","year":"2016a","journal-title":"Sci Rep"},{"issue":"4","key":"pntd.0010324.ref012","doi-asserted-by":"crossref","first-page":"e0004677","DOI":"10.1371\/journal.pntd.0004677","article-title":"The wMel strain of Wolbachia reduces transmission of chikungunya virus in Aedes aegypti","volume":"10","author":"MT Aliota","year":"2016b","journal-title":"PLoS Negl Trop Dis"},{"key":"pntd.0010324.ref013","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1016\/j.chom.2016.04.021","article-title":"Wolbachia blocks currently circulating Zika virus isolates in Brazilian Aedes aegypti mosquitoes","volume":"19","author":"HL Dutra","year":"2016","journal-title":"Cell Host Microbe"},{"key":"pntd.0010324.ref014","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1111\/aen.12127","article-title":"Rear and release: a new paradigm for dengue control","volume":"53","author":"S Ritchie","year":"2014","journal-title":"Aust Entomol"},{"key":"pntd.0010324.ref015","volume-title":"Dengue and Zika: Control and Antiviral Treatment Strategies. Advances in Experimental Medicine and Biology","author":"SL O\u2019Neill","year":"2018"},{"key":"pntd.0010324.ref016","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.pt.2017.11.011","article-title":"Mission Accomplished? We need a guide to the \u201bpost release\u02bc world of Wolbachia for Aedes-borne disease control","volume":"34","author":"SA Ritchie","year":"2018","journal-title":"Trends Parasitol"},{"key":"pntd.0010324.ref017","doi-asserted-by":"crossref","first-page":"e000777","DOI":"10.1371\/journal.pntd.0007771","article-title":"Combined sterile insect technique and incompatible insect technique: the first proof-of-concept to suppress Aedes aegypti vector populations in semi-rural settings in Thailand","volume":"13","author":"P Kittayapong","year":"2019","journal-title":"Plos Negl Trop Dis"},{"key":"pntd.0010324.ref018","doi-asserted-by":"crossref","first-page":"1296","DOI":"10.1093\/jme\/tjz051","article-title":"Localized control of Aedes aegypti (Diptera: Culicidae) in Miami, FL, via inundative releases of Wolbachia-infected male mosquitoes","volume":"56","author":"JW Mains","year":"2019","journal-title":"J Med Entomol"},{"key":"pntd.0010324.ref019","first-page":"36","volume":"2","author":"SL O\u2019Neill","year":"2019","journal-title":"Scaled deployment of Wolbachia to protect the community from dengue and other Aedes transmitted arboviruses [version 3; peer review: 2 approved] Gates Open Res"},{"key":"pntd.0010324.ref020","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.12688\/gatesopenres.13061.1","article-title":"Establishment of wMel Wolbachia in Aedes aegypti mosquitoes and reduction of local dengue transmission in Cairns and surrounding locations in northern Queensland, Australia","volume":"3","author":"P Ryan","year":"2019","journal-title":"Gates Open Res"},{"key":"pntd.0010324.ref021","doi-asserted-by":"crossref","first-page":"50","DOI":"10.12688\/gatesopenres.13122.1","article-title":"Reduced dengue incidence following deployments of Wolbachia-infected Aedes aegypti in Yogyakarta, Indonesia: a quasi-experimental trial using controlled interrupted time series analysis","volume":"4","author":"C Indriani","year":"2020","journal-title":"Gates Open Res"},{"issue":"1","key":"pntd.0010324.ref022","doi-asserted-by":"crossref","first-page":"10039","DOI":"10.1038\/s41598-021-89409-8","article-title":"Reduced competence to arboviruses following the sustainable invasion of Wolbachia into native Aedes aegypti from Southeastern Brazil","volume":"11","author":"J Gesto","year":"2021","journal-title":"Sci Rep"},{"issue":"24","key":"pntd.0010324.ref023","first-page":"4241","volume":"29","author":"WA Nazni","year":"2019","journal-title":"Establishment of Wolbachia Strain wAlbB in Malaysian Populations of Aedes aegypti for Dengue Control Curr Biol"},{"key":"pntd.0010324.ref024","unstructured":"worldmosquitoprogram.org [Internet]. Mexico: World Mosquito Program (WMP); c2021. [cited 2021 Jun 18]. Available from: https:\/\/www.worldmosquitoprogram.org\/es\/avances-nivel-mundial\/mexico\/la-paz."},{"issue":"4","key":"pntd.0010324.ref025","doi-asserted-by":"crossref","first-page":"1817","DOI":"10.1093\/jme\/tjab052","article-title":"Abundance and Seasonality of Aedes aegypti (Diptera: Culicidae) in Two Suburban Localities of South Mexico, With Implications for Wolbachia (Rickettsiales: Rickettsiaceae)-Carrying Male Releases for Population Suppression","volume":"58","author":"A Che-Mendoza","year":"2021","journal-title":"J Med Entomol"},{"issue":"8","key":"pntd.0010324.ref026","doi-asserted-by":"crossref","first-page":"e0135194","DOI":"10.1371\/journal.pone.0135194","article-title":"Combining the Sterile Insect Technique with Wolbachia-Based Approaches: II- A Safer Approach to Aedes albopictus Population Suppression Programmes, Designed to Minimize the Consequences of Inadvertent Female Release","volume":"10","author":"D Zhang","year":"2015","journal-title":"PLoS One"},{"issue":"3","key":"pntd.0010324.ref027","doi-asserted-by":"crossref","first-page":"e0151864","DOI":"10.1371\/journal.pone.0151864","article-title":"Combining the Sterile Insect Technique with the Incompatible Insect Technique: III-Robust Mating Competitiveness of Irradiated Triple Wolbachia-Infected Aedes albopictus Males under Semi-Field Conditions","volume":"11","author":"D Zhang","year":"2016","journal-title":"PLoS One"},{"key":"pntd.0010324.ref028","doi-asserted-by":"crossref","first-page":"1531","DOI":"10.1007\/s10340-021-01340-z","article-title":"Quality control of long-term mass-reared Aedes albopictus for population suppression","volume":"94","author":"Y Li","year":"2021","journal-title":"J Pest Sci"},{"key":"pntd.0010324.ref029","doi-asserted-by":"crossref","first-page":"507","DOI":"10.4269\/ajtmh.15-0608","article-title":"Fitness of wAlbB Wolbachia infection in Aedes aegypti: parameter estimates in an outcrossed background and potential for population invasion","volume":"94","author":"JK Axford","year":"2016","journal-title":"Am J Trop Med Hyg"},{"issue":"1","key":"pntd.0010324.ref030","doi-asserted-by":"crossref","first-page":"e1006006","DOI":"10.1371\/journal.ppat.1006006","article-title":"Wolbachia Infections in Aedes aegypti Differ Markedly in Their Response to Cyclical Heat Stress","volume":"13","author":"PA Ross","year":"2017","journal-title":"PLoS Pathog"},{"key":"pntd.0010324.ref031","unstructured":"dof.gob.mx [Internet]. M\u00e9xico: Diario Oficial de la Federaci\u00f3n. Norma Oficial Mexicana NOM-032-SSA2-2014, Para la vigilancia epidemiol\u00f3gica, promoci\u00f3n, prevenci\u00f3n y control de las enfermedades transmitidas por vectores; c2015. [cited 2021 Feb 15]. Available from: http:\/\/www.dof.gob.mx\/nota_detalle.php?codigo = 5389045&fecha = 16\/04\/2015."},{"issue":"4","key":"pntd.0010324.ref032","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.pt.2020.01.004","article-title":"Phased Conditional Approach for Mosquito Management Using Sterile Insect Technique","volume":"36","author":"J Bouyer","year":"2020","journal-title":"Trends Parasitol"},{"key":"pntd.0010324.ref033","article-title":"Guidance framework for testing the sterile insect technique (SIT) as a vector control tool against aedes-borne diseases","author":"IAEA\/WHO","year":"2020","journal-title":"Geneva & Vienna: World Health Organization and the International Atomic Energy Agency"},{"key":"pntd.0010324.ref034","doi-asserted-by":"crossref","first-page":"191","DOI":"10.3390\/insects12030191","article-title":"Sterile Insect Technique (SIT) against Aedes Species Mosquitoes: A Roadmap and Good Practice Framework for Designing, Implementing and Evaluating Pilot Field Trials","volume":"12","author":"CF Oliva","year":"2021","journal-title":"Insects"},{"key":"pntd.0010324.ref035","unstructured":"smn.conagua.gob.mx [Internet]. M\u00e9xico: Normales climatol\u00f3gicas per\u00edodo: 1981\u20132010. ESTACI\u00d3N: 00031019 MERIDA (OBS) CONAGUA; c2020. [cited 2021 Jan 8]. Available from: https:\/\/smn.conagua.gob.mx\/es\/climatologia\/informacion-climatologica\/informacion-estadistica-climatologica."},{"key":"pntd.0010324.ref036","volume-title":"Progress and prospects for the use of genetically modified mosquitoes to inhibit disease transmission","author":"World Health Organization","year":"2010"},{"issue":"6","key":"pntd.0010324.ref037","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1603\/033.046.0602","article-title":"A new, cost-effective, battery-powered aspirator for adult mosquito collections","volume":"46","author":"GM Vazquez-Prokopec","year":"2009","journal-title":"J Med Entomol"},{"key":"pntd.0010324.ref038","unstructured":"www.gob.mx [Internet]. M\u00e9xico: Productos recomendados por el CENAPRECE para el combate de insectos vectores de enfermedades a partir de 2020; c2020. [cited 2021 Jun 22]. Available from: https:\/\/www.gob.mx\/cms\/uploads\/attachment\/file\/546678\/Productos_Recomendados_por_el_CENAPRECE_para_el_Combate_de_los_Insectos_Vectores_2020.pdf."},{"issue":"6","key":"pntd.0010324.ref039","doi-asserted-by":"crossref","first-page":"e0005656","DOI":"10.1371\/journal.pntd.0005656","article-title":"Deltamethrin resistance in Aedes aegypti results in treatment failure in Merida, Mexico","volume":"11","author":"G Vazquez-Prokopec","year":"2017","journal-title":"PLoS Negl Trop Dis"},{"issue":"1","key":"pntd.0010324.ref040","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1186\/s13071-020-04093-3","article-title":"Impact of deltamethrin selection on kdr mutations and insecticide detoxifying enzymes in Aedes aegypti from Mexico","volume":"13","author":"Y Contreras-Perera","year":"2020","journal-title":"Parasit Vectors"},{"key":"pntd.0010324.ref041","unstructured":"www.gob.mx [Internet]. M\u00e9xico: CENAPRECE. Gu\u00eda metodol\u00f3gica para vigilancia entomol\u00f3gica con ovitrampas; c2015a. [cited 2021 May 21]. Available from: https:\/\/www.gob.mx\/cms\/uploads\/attachment\/file\/43289\/GuiaMetodologicaVigilanciaEntomologicaOvitrampas.pdf."},{"issue":"8","key":"pntd.0010324.ref042","doi-asserted-by":"crossref","first-page":"e70231","DOI":"10.1371\/journal.pone.0070231","article-title":"Nation-wide, web-based, geographic information system for the integrated surveillance and control of dengue fever in Mexico","volume":"8","author":"JE Hern\u00e1ndez-\u00c1vila","year":"2013","journal-title":"PLoS ONE"},{"key":"pntd.0010324.ref043","unstructured":"www.cenaprece.salud.gob.mx [Internet]. CENAPRECE. Gu\u00eda para la instalaci\u00f3n y mantenimiento del insectario de Aedes aegypti (Diptera: Culicidae); c2015b. [cited 2021 May21]. Available from: http:\/\/www.cenaprece.salud.gob.mx\/programas\/interior\/vectores\/descargas\/pdf\/GuiaInstalacionMantenimientoInsectarioAedesAegypti.pdf."},{"key":"pntd.0010324.ref044","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1111\/eea.12892","article-title":"Aedes aegypti lines for combined sterile insect technique and incompatible insect technique applications: the importance of host genomic background","volume":"168","author":"DO Carvalho","year":"2020","journal-title":"Entomol Exp Appl"},{"key":"pntd.0010324.ref045","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1186\/s13071-017-2511-z","article-title":"Establishment of a medium-scale mosquito facility: optimization of the larval mass-rearing unit for Aedes albopictus (Diptera: Culicidae)","volume":"10","author":"D. Zhang","year":"2017","journal-title":"Parasit Vectors"},{"key":"pntd.0010324.ref046","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1186\/s13071-018-2750-7","article-title":"Establishment of a medium-scale mosquito facility: tests on mass production cages for Aedes albopictus (Diptera: Culicidae)","volume":"11","author":"D Zhang","year":"2018","journal-title":"Parasit Vectors"},{"issue":"3","key":"pntd.0010324.ref047","first-page":"144","article-title":"A mechanical device for separating the developmental stages, sexes and species of mosquitoes","volume":"19","author":"RW Fay","year":"1959","journal-title":"Mosq News"},{"issue":"1","key":"pntd.0010324.ref048","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1186\/s13071-019-3398-7","article-title":"Use of mechanical and behavioural methods to eliminate female Aedes aegypti and Aedes albopictus for sterile insect technique and incompatible insect technique applications","volume":"12","author":"N Gunathilaka","year":"2019","journal-title":"Parasit Vectors"},{"key":"pntd.0010324.ref049","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1038\/nbt.2350","article-title":"Successful suppression of a field mosquito population by sustained release of engineered male mosquitoes","volume":"30","author":"AF Harris","year":"2012","journal-title":"Nat Biotechnol"},{"key":"pntd.0010324.ref050","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1007\/1-4020-4051-2_2","volume-title":"Sterile Insect Technique. Principles and Practice in Area-wide Integrated Pest Management","author":"W. Klassen","year":"2005"},{"issue":"Suppl 2","key":"pntd.0010324.ref051","doi-asserted-by":"crossref","first-page":"S2","DOI":"10.1186\/1475-2875-8-S2-S2","article-title":"Historical applications of induced sterilization in field populations of mosquitoes","volume":"8","author":"DA Dame","year":"2009","journal-title":"Malar J"},{"key":"pntd.0010324.ref052","volume-title":"The basic principles of insect population suppression and management","author":"EF Knipling","year":"1979"},{"key":"pntd.0010324.ref053","doi-asserted-by":"crossref","first-page":"624","DOI":"10.1603\/ME12201","article-title":"Novel Estimates of Aedes aegypti (Diptera: Culicidae) Population Size and Adult Survival Based on Wolbachia Releases","volume":"50","author":"S Ritchie","year":"2013","journal-title":"J Med Entomol"},{"issue":"3","key":"pntd.0010324.ref054","doi-asserted-by":"crossref","first-page":"210","DOI":"10.2987\/18-6797.1","article-title":"New record of Aedes albopictus in a suburban area of Merida, Yucatan, Mexico","volume":"35","author":"Y Contreras-Perera","year":"2019","journal-title":"J Am Mosq Control Assoc"},{"issue":"1","key":"pntd.0010324.ref055","doi-asserted-by":"crossref","first-page":"153","DOI":"10.7705\/biomedica.5525","article-title":"Detecci\u00f3n de Aedes (Stegomyia) albopictus (Skuse) en ovitrampas en M\u00e9rida, M\u00e9xico","volume":"41","author":"G Gonz\u00e1lez-Olvera","year":"2021","journal-title":"Biom\u00e9dica"},{"key":"pntd.0010324.ref056","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1007\/978-0-387-68636-3_4","volume-title":"In: Design and Analysis of Vaccine Studies","author":"ME Halloran","year":"2010"},{"key":"pntd.0010324.ref057","unstructured":"prvectorcontrol.org [Internet]. Puerto Rico: Puerto Rico Vector Control Unit (PRVCU); c2021 [cited 2021 May 27]. Available from: https:\/\/prvectorcontrol.org\/."},{"key":"pntd.0010324.ref058","unstructured":"oecd-nea.org [Internet]. Nuclear Energy Agency (NEA); c2021 [cited 2021 May 27]. Available from: https:\/\/www.oecd-nea.org\/."},{"issue":"2","key":"pntd.0010324.ref059","doi-asserted-by":"crossref","first-page":"e0009179","DOI":"10.1371\/journal.pntd.0009179","article-title":"Infertility and fecundity loss of Wolbachia-infected Aedes aegypti hatched from quiescent eggs is expected to alter invasion dynamics","volume":"15","author":"M-J Lau","year":"2021","journal-title":"PloS Negl Trop Dis"},{"key":"pntd.0010324.ref060","volume-title":"Dengue Virus. Curr Top Microbiol Immunol","author":"TW Scott","year":"2010"},{"issue":"1","key":"pntd.0010324.ref061","doi-asserted-by":"crossref","first-page":"e0008972","DOI":"10.1371\/journal.pntd.0008972","article-title":"Natural arbovirus infection rate and detectability of indoor female Aedes aegypti from M\u00e9rida, Yucat\u00e1n, Mexico","volume":"15","author":"OD Kirstein","year":"2021","journal-title":"PloS Negl Trop Dis"},{"key":"pntd.0010324.ref062","volume-title":"Evaluation of Innovative Strategies for Aedes aegypti Control: Challenges for their Introduction and Impact Assessment","author":"Pan American Health Organization","year":"2019"},{"issue":"3","key":"pntd.0010324.ref063","doi-asserted-by":"crossref","first-page":"275","DOI":"10.2987\/moco-31-03-275-277.1","article-title":"Arbovirus surveillance and first report of chikungunya virus in wild populations of Aedes aegypti from Guerrero, Mexico","volume":"31","author":"F Dzul-Manzanilla","year":"2015","journal-title":"J Am Mosq Control Assoc"},{"issue":"2","key":"pntd.0010324.ref064","first-page":"141","article-title":"Evidence of vertical transmission and co-circulation of chikungunya and dengue viruses in field populations of Aedes aegypti (L.) from Guerrero, Mexico","volume":"110","author":"F Dzul-Manzanilla","year":"2016","journal-title":"Trans R Soc Trop Med Hyg"},{"issue":"7","key":"pntd.0010324.ref065","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1093\/trstmh\/trx056","article-title":"Detection of Zika virus in Aedes mosquitoes from Mexico","volume":"111","author":"H Huerta","year":"2017","journal-title":"Trans R Soc Trop Med Hyg"},{"issue":"7","key":"pntd.0010324.ref066","doi-asserted-by":"crossref","first-page":"e0003864","DOI":"10.1371\/journal.pntd.0003864","article-title":"Suppression of a Field Population of Aedes aegypti in Brazil by Sustained Release of Transgenic Male Mosquitoes","volume":"9","author":"DO Carvalho","year":"2015","journal-title":"PloS Negl Trop Dis"},{"issue":"2","key":"pntd.0010324.ref067","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1111\/1744-7917.12291","article-title":"Satyrization and satyrization-resistance in competitive displacements of invasive mosquito species","volume":"23","author":"IE Bargielowski","year":"2016","journal-title":"Insect Sci"},{"key":"pntd.0010324.ref068","volume-title":"Fifth meeting of the vector control advisory group","author":"World Health Organization","year":"2016"},{"issue":"5","key":"pntd.0010324.ref069","doi-asserted-by":"crossref","first-page":"e277","DOI":"10.1016\/S2542-5196(21)00030-9","article-title":"Identifying urban hotspots of dengue, chikungunya, and Zika transmission in Mexico to support risk stratification efforts: a spatial analysis","volume":"5","author":"F Dzul-Manzanilla","year":"2021","journal-title":"Lancet Planet Health"}],"container-title":["PLOS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0010324","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,3]],"date-time":"2023-02-03T10:54:51Z","timestamp":1675421691000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0010324"}},"subtitle":[],"editor":[{"given":"Geraldine Marie","family":"Foster","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2022,4,26]]},"references-count":69,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2022,4,26]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0010324","relation":{},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,4,26]]}}}