{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T02:11:40Z","timestamp":1787623900717,"version":"build-2736575974"},"update-to":[{"DOI":"10.1371\/journal.pntd.0010392","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2022,5,20]],"date-time":"2022-05-20T00:00:00Z","timestamp":1653004800000}}],"reference-count":48,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2022,5,10]],"date-time":"2022-05-10T00:00:00Z","timestamp":1652140800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["155904\/2019-1"],"award-info":[{"award-number":["155904\/2019-1"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>Q fever and brucellosis are zoonoses that cause fever and other systemic clinical signs in humans; their occurrences are neglected and the differential diagnosis for some diseases is disregarded. This study aimed to investigate the seropositivity for<jats:italic>Coxiella burnetii<\/jats:italic>and<jats:italic>Brucella<\/jats:italic>spp. antibodies in patients suspected of dengue from 38 municipalities in the state of S\u00e3o Paulo, Brazil. The samples (n = 604) were obtained by convenience from the Adolfo Lutz Institute serum bank. Sera were subjected to an indirect immunofluorescence assay (IFA) using<jats:italic>in-house<\/jats:italic>and commercial diagnostic protocols to evaluate<jats:italic>C<\/jats:italic>.<jats:italic>burnetii<\/jats:italic>positivity. For<jats:italic>Brucella<\/jats:italic>spp., sera were subjected to rapid plate serum agglutination with buffered acidified antigen (AAT), slow tube serum agglutination (SAL), and 2-mercaptoethanol (2-ME) techniques. Associations and statistical inferences of the results were performed by logistic regression according to the clinical and demographic variables collected from the patients. Statistical analyses were performed using Statistical Analysis Software (SAS) and associations were considered when p value was &lt;0.05. In all, 129 patients showed positive results for Q fever, indicating a seropositivity of 21.4% (95% CI 18.15\u201324.85). Patients with 14\u201320 days of symptoms had 2.12 (95% CI 1.34\u20133.35) times more chances of being seropositive for Q fever than patients with 7\u201313 days, and patients with 21\u201327 days of fever had 2.62 (95% CI 1.27\u20135.41) times more chances of being seropositive for Q fever than patients with 7\u201313 days. For the other variables analyzed, there were no significant associations between the groups. No positivity for brucellosis was observed. This is the most comprehensive study of people seropositive for Q fever in S\u00e3o Paulo state and provides additional data for the medical community in Brazil. It is suggested that Q fever may be an important differential diagnosis of febrile illnesses in the region, demanding the government\u2019s attention and investment in health.<\/jats:p>","DOI":"10.1371\/journal.pntd.0010392","type":"journal-article","created":{"date-parts":[[2022,5,10]],"date-time":"2022-05-10T18:01:23Z","timestamp":1652205683000},"page":"e0010392","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":25,"title":["Seropositivity for Coxiella burnetii in suspected patients with dengue in S\u00e3o Paulo state, Brazil"],"prefix":"10.1371","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1178-5643","authenticated-orcid":true,"given":"Danilo Alves de","family":"Fran\u00e7a","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7886-1570","authenticated-orcid":true,"given":"Mateus de Souza Ribeiro","family":"Mioni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1995-7243","authenticated-orcid":true,"given":"Felipe","family":"Fornazari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2480-9042","authenticated-orcid":true,"given":"Ana \u00cdris de Lima","family":"Dur\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5365-4329","authenticated-orcid":true,"given":"Marcos Vinicius Ferreira","family":"Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0118-6164","authenticated-orcid":true,"given":"F\u00e1bio Sossai","family":"Possebon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8312-7397","authenticated-orcid":true,"given":"Virg\u00ednia Bodel\u00e3o","family":"Richini-Pereira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5127-0762","authenticated-orcid":true,"given":"Helio","family":"Langoni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6540-7157","authenticated-orcid":true,"given":"Jane","family":"Megid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,5,10]]},"reference":[{"issue":"9","key":"pntd.0010392.ref001","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.tifs.2017.11.004","article-title":"Q fever and prevalence of Coxiella burnetii in milk","volume":"71","author":"A Pexara","year":"2018","journal-title":"Trends Food Sci Technol"},{"issue":"4","key":"pntd.0010392.ref002","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/S1473-3099(05)70052-9","article-title":"Natural history and pathophysiology of Q fever","volume":"5","author":"D Raoult","year":"2005","journal-title":"Lancet Infect Dis"},{"issue":"1","key":"pntd.0010392.ref003","doi-asserted-by":"crossref","first-page":"127","DOI":"10.11606\/issn.2358-792X.v7i1p127-131","article-title":"Investiga\u00e7\u00f5es sobre a febre \u201cq\u201d em S\u00e3o Paulo\u2014estudo sorol\u00f3gico em oper\u00e1rios de um frigor\u00edfico.","volume":"7","author":"H Brand\u00e3o","year":"1953","journal-title":"Arq Fac Hig Saude Publica Univ Sao Paulo"},{"key":"pntd.0010392.ref004","first-page":"199","article-title":"Febre Q no Rio de Janeiro.","volume":"6","author":"J Travassos","year":"1954","journal-title":"Ci\u00eanc Cult."},{"issue":"1","key":"pntd.0010392.ref005","doi-asserted-by":"crossref","first-page":"167","DOI":"10.11606\/issn.2358-792X.v9i1-2p167-180","article-title":"Investiga\u00e7\u00e3o sobre a febre Q em S\u00e3o Paulo: estudo em tratadores de gado e em bovinos.","volume":"9","author":"LB Valle","year":"1955","journal-title":"Arq Fac Hig Saude Publica Univ Sao Paulo"},{"key":"pntd.0010392.ref006","first-page":"424","article-title":"Serological evidence of Q fever in herders in Brazil.","volume":"60","author":"DR Borges","year":"1962","journal-title":"Rev Paul Med"},{"key":"pntd.0010392.ref007","first-page":"255","article-title":"Q fever study in S\u00e3o Paulo: III. 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