{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T20:01:18Z","timestamp":1791576078542,"version":"4.3.4"},"update-to":[{"DOI":"10.1371\/journal.pntd.0010523","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000}}],"reference-count":23,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2022,6,21]],"date-time":"2022-06-21T00:00:00Z","timestamp":1655769600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"ANR Agence Nationale de la Recherche","award":["Mitobothrops Grant ANR-18-CE17-0026-01"],"award-info":[{"award-number":["Mitobothrops Grant ANR-18-CE17-0026-01"]}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"crossref","award":["ANR-18-CE17-0026-03"],"award-info":[{"award-number":["ANR-18-CE17-0026-03"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Introduction<\/jats:title>\n<jats:p>Envenomations by <jats:italic>Bothrops<\/jats:italic> snakebites can induce overwhelming systemic inflammation ultimately leading to multiple organ system failure and death. Release of damage-associated molecular pattern molecules (DAMPs), in particular of mitochondrial origin, has been implicated in the pathophysiology of the deregulated innate immune response.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Objective<\/jats:title>\n<jats:p>To test whether whole <jats:italic>Bothrops lanceolatus<\/jats:italic> venom would induce mitochondrial dysfunction and DAMPs release in human heart preparations.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Methods<\/jats:title>\n<jats:p>Human atrial trabeculae were obtained during cannulation for cardiopulmonary bypass from patients who were undergoing routine coronary artery bypass surgery. Cardiac fibers were incubated with vehicle and whole <jats:italic>Bothrops lanceolatus<\/jats:italic> venom for 24hr before high-resolution respirometry, mitochondrial membrane permeability evaluation and quantification of mitochondrial DNA.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec004\">\n<jats:title>Results<\/jats:title>\n<jats:p>Compared with vehicle, incubation of human cardiac muscle with whole <jats:italic>Bothrops lanceolatus<\/jats:italic> venom for 24hr impaired respiratory control ratio and mitochondrial membrane permeability. Levels of mitochondrial DNA increased in the medium of cardiac cell preparation incubated with venom of <jats:italic>Bothrops lanceolatus<\/jats:italic>.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec005\">\n<jats:title>Conclusion<\/jats:title>\n<jats:p>Our study suggests that whole venom of <jats:italic>Bothrops lanceolatus<\/jats:italic> impairs mitochondrial oxidative phosphorylation capacity and increases mitochondrial membrane permeability. Cardiac mitochondrial dysfunction associated with mitochondrial DAMPs release may alter myocardium function and engage the innate immune response, which may both participate to the cardiotoxicity occurring in patients with severe envenomation.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pntd.0010523","type":"journal-article","created":{"date-parts":[[2022,6,21]],"date-time":"2022-06-21T17:38:01Z","timestamp":1655833081000},"page":"e0010523","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":12,"title":["Bothrops lanceolatus snake venom impairs mitochondrial respiration and induces DNA release in human heart 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