{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T16:34:34Z","timestamp":1788366874851,"version":"build-2803163510"},"update-to":[{"DOI":"10.1371\/journal.pntd.0010535","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2022,7,22]],"date-time":"2022-07-22T00:00:00Z","timestamp":1658448000000}}],"reference-count":48,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2022,7,7]],"date-time":"2022-07-07T00:00:00Z","timestamp":1657152000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Inova Fiocruz","award":["VPPCB-007-FIO-18-2-72"],"award-info":[{"award-number":["VPPCB-007-FIO-18-2-72"]}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["311539\/2020-3"],"award-info":[{"award-number":["311539\/2020-3"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"publisher","award":["E-26\/203.031\/2018"],"award-info":[{"award-number":["E-26\/203.031\/2018"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>\n                    A recurring question concerning\n                    <jats:italic>Trypanosoma cruzi<\/jats:italic>\n                    DNA detection\/quantification is related to the fact that DNA amplification, by itself, does not differentiate between viable or dead parasites. On the other hand, RNA can be considered a potential molecular marker of pathogens viability. Herein, we developed a quantitative real-time PCR with reverse Transcription (RT-qPCR) to quantify viable\n                    <jats:italic>T<\/jats:italic>\n                    .\n                    <jats:italic>cruzi<\/jats:italic>\n                    in artificially infected\n                    <jats:italic>Rhodnius prolixus<\/jats:italic>\n                    whilst evaluating differences between DNA and mRNA quantification along the insect midgut during 5, 9, 15 and 29 days after feeding. The RT-qPCR presented an improved performance with linearities ranging from 10\n                    <jats:sup>7<\/jats:sup>\n                    to 10\n                    <jats:sup>2<\/jats:sup>\n                    parasites equivalents and 3 to 0.0032 intestine unit equivalents, and efficiencies of 100.3% and 102.8% for both\n                    <jats:italic>T<\/jats:italic>\n                    .\n                    <jats:italic>cruzi<\/jats:italic>\n                    and triatomine targets, respectively. Comparing both RT-qPCR and qPCR, we confirmed that RNA is faster degraded, no longer being detected at day 1 after parasite lysis, while DNA detection was stable, with no decrease in parasite load over the days, even after parasite lysis. We also observed statistical differences between the quantification of the parasite load by DNA and by RNA on day 15 after feeding of experimentally infected\n                    <jats:italic>R<\/jats:italic>\n                    .\n                    <jats:italic>prolixus<\/jats:italic>\n                    . When assessing different portions of the digestive tract, by RT-qPCR, we could detect a statistically significant reduction in the parasite amount in the anterior midgut. Oppositely, there was a statistically significant increase of the parasite load in the hindgut. In conclusion, for this study parasite\u2019s viability in R. prolixus digestive tract were assessed targeting\n                    <jats:italic>T<\/jats:italic>\n                    .\n                    <jats:italic>cruzi<\/jats:italic>\n                    mRNA. In addition, differences between DNA and RNA detection observed herein, raise the possibility that RNA is a potential molecular viability marker, which could contribute to understanding the dynamics of the parasite infection in invertebrate hosts.\n                  <\/jats:p>","DOI":"10.1371\/journal.pntd.0010535","type":"journal-article","created":{"date-parts":[[2022,7,7]],"date-time":"2022-07-07T13:41:46Z","timestamp":1657201306000},"page":"e0010535","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":9,"title":["RNA as a feasible marker of Trypanosoma cruzi viability during the parasite interaction with the triatomine vector Rhodnius prolixus (Hemiptera, Triatominae)"],"prefix":"10.1371","volume":"16","author":[{"given":"Paula","family":"Finamore-Araujo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel Lucio","family":"Silva da Fonseca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cec\u00edlia Stahl","family":"Vieira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniele Pereira","family":"de Castro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5340-8697","authenticated-orcid":true,"given":"Otacilio Cruz","family":"Moreira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,7,7]]},"reference":[{"key":"pntd.0010535.ref001","author":"Organization WH","year":"2020","journal-title":"Chagas disease (also known as American trypanosomiasis) [Internet]"},{"issue":"7","key":"pntd.0010535.ref002","doi-asserted-by":"crossref","first-page":"856","DOI":"10.1590\/0074-0276140236","article-title":"Ecoepidemiology, Short history and control of chagas disease in the endemic countries and the new challenge for non-endemic countries","volume":"109","author":"JR Coura","year":"2014","journal-title":"Mem Inst Oswaldo Cruz"},{"issue":"SUPPL. 1","key":"pntd.0010535.ref003","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1590\/S0074-02762009000900007","article-title":"Elimination of chagas disease transmission: Perspectives","volume":"104","author":"JCP Dias","year":"2009","journal-title":"Mem Inst Oswaldo Cruz"},{"issue":"1","key":"pntd.0010535.ref004","first-page":"1","article-title":"The multiple and complex and changeable scenarios of the Trypanosoma cruzi transmission cycle in the sylvatic environment","volume":"151","author":"AM Jansen","year":"2015","journal-title":"Acta Trop [Internet]."},{"key":"pntd.0010535.ref005","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.actatropica.2017.09.017","article-title":"Trypanosoma cruzi genetic diversity: Something new for something known about Chagas disease manifestations, serodiagnosis and drug sensitivity","volume":"184","author":"B. 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