{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T11:30:11Z","timestamp":1767180611230,"version":"build-2238731810"},"update-to":[{"DOI":"10.1371\/journal.pntd.0010679","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2022,8,29]],"date-time":"2022-08-29T00:00:00Z","timestamp":1661731200000}}],"reference-count":54,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2022,8,17]],"date-time":"2022-08-17T00:00:00Z","timestamp":1660694400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007338","name":"Foundation for Barnes-Jewish Hospital","doi-asserted-by":"publisher","award":["615735"],"award-info":[{"award-number":["615735"]}],"id":[{"id":"10.13039\/100007338","id-type":"DOI","asserted-by":"publisher"}]},{"name":"WU Institute of Clinical and Translational Sciences","award":["NCATS UL1 TR000448"],"award-info":[{"award-number":["NCATS UL1 TR000448"]}]},{"name":"Mass Spectrometry Research Resource","award":["NIGMS P41 GM103422; R24GM136766"],"award-info":[{"award-number":["NIGMS P41 GM103422; R24GM136766"]}]},{"name":"Siteman Comprehensive Cancer Center Support Grant","award":["NCI P30 CA091842"],"award-info":[{"award-number":["NCI P30 CA091842"]}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>\n                    <jats:italic>Paragonimus kellicotti<\/jats:italic>\n                    is a zoonotic lung fluke infection, the agent of North American paragonimiasis, and an excellent model for other\n                    <jats:italic>Paragonimus<\/jats:italic>\n                    infections. The excretory\/secretory proteins (ESP) released by parasites and presented at the parasite-host interface are frequently proposed to be useful targets for drugs and\/or vaccines In vitro culture conditions may alter ESP compared to those produced in vivo. In order to investigate ESPs produced in vivo we took advantage of the fact that adult\n                    <jats:italic>P<\/jats:italic>\n                    .\n                    <jats:italic>kellicotti<\/jats:italic>\n                    reproduce in the lungs of experimentally infected gerbils in tissue cysts. We performed a mass-spectrometric analysis of adult\n                    <jats:italic>P<\/jats:italic>\n                    .\n                    <jats:italic>kellicotti<\/jats:italic>\n                    soluble somatic protein (SSPs) extracts, excreted\/secreted proteins (ESPs) produced by adult worms during\n                    <jats:italic>in vitro<\/jats:italic>\n                    culture, and lung cyst fluid proteins (CFPs) from experimentally infected gerbils. We identified 2,137\n                    <jats:italic>P<\/jats:italic>\n                    .\n                    <jats:italic>kellicotti<\/jats:italic>\n                    proteins that were present in at least two of three biological replicates and supported by at least two peptides. Among those were 1,914 proteins found in SSP, 947 in ESP and 37 in CFP.\n                    <jats:italic>In silico<\/jats:italic>\n                    analysis predicted that only 141 of the total 2,137 proteins were secreted via classical or non-classical pathways. The most abundant functional categories in SSP were storage and oxidative metabolism. The most abundant categories in ESP were proteins related to metabolism and signal transduction. The 37 parasite-related proteins in CFP belonged to 11 functional categories. The largest groups were proteins with unknown function, cytoskeletal proteins and proteasome machinery. 29 of these 37 proteins were shared among all three sample types. To our knowledge, this is the first study that compares\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    ESP for any\n                    <jats:italic>Paragonimus<\/jats:italic>\n                    species. This study has provided new insights into ESPs of food-borne trematodes that are produced and released\n                    <jats:italic>in vivo<\/jats:italic>\n                    . Proteins released at the host-parasite interface may help the parasite evade host immunity and may represent new targets for novel treatments or diagnostic tests for paragonimiasis.\n                  <\/jats:p>","DOI":"10.1371\/journal.pntd.0010679","type":"journal-article","created":{"date-parts":[[2022,8,17]],"date-time":"2022-08-17T13:43:32Z","timestamp":1660743812000},"page":"e0010679","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":7,"title":["Comparative proteomics of adult Paragonimus kellicotti excretion\/secretion products released in vitro or present in the lung cyst nodule"],"prefix":"10.1371","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9558-4157","authenticated-orcid":true,"given":"Lucia S.","family":"Di Maggio","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kurt C.","family":"Curtis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petra","family":"Erdmann-Gilmore","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert S. 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