{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T23:47:14Z","timestamp":1788824834441,"version":"build-2803163510"},"reference-count":53,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2022,10,13]],"date-time":"2022-10-13T00:00:00Z","timestamp":1665619200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Brazilian Council for Scientific and Technological Development","award":["405205\/2016-3"],"award-info":[{"award-number":["405205\/2016-3"]}]},{"name":"Financier of Studies and Projects","award":["01.16.0057.00"],"award-info":[{"award-number":["01.16.0057.00"]}]},{"name":"Amazonas State Research Support Foundation","award":["134\/2018"],"award-info":[{"award-number":["134\/2018"]}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:sec id=\"sec001\"><jats:title>Background<\/jats:title><jats:p>In the clinical course of diseases such as arboviruses, skin rashes may appear, as is often seen in other infectious diseases. The aim of this study was to estimate the prevalence of arboviruses and other infectious causes of skin rash in a tertiary health unit in Manaus, Amazonas state, Western Brazilian Amazon<\/jats:p><\/jats:sec><jats:sec id=\"sec002\"><jats:title>Methodology\/Principal findings<\/jats:title><jats:p>This was a cross-sectional study of patients presenting with rash who sought care at<jats:italic>Funda\u00e7\u00e3o de Medicina Tropical Dr<\/jats:italic>.<jats:italic>Heitor Vieira Dourado<\/jats:italic>(FMT-HVD) from February 2018 to May 2019. Individuals of either gender, aged over 18 years, were invited to participate voluntarily. Infection by Zika virus (ZIKV), dengue virus (DENV), chikungunya virus (CHIKV), Mayaro virus (MAYV), Oropouche virus (OROV) and measles was evaluated using RT-qPCR (real-time polymerase chain reaction). Immunodiagnostic tests for EBV, CMV, HIV, syphilis, rubella and measles were also performed. A total of 340 participants were included, most were female (228, 67.1%) with an average age of 36.5 years (SD \u00b1 12.2 years). The highest prevalence was of ZIKV monoinfections (65.3%, 222\/340), followed by DENV (0.9%, 3\/340) and CHIKV infection (0.3%, 1\/340). No cases of MAYV, OROV or rubella were found. Other causes of skin rash were detected: measles (2.9%, 10\/340), parvovirus B19 (0.9% 3\/340), HIV (0.3%, 1\/340) and syphilis 0.6% (2\/340). The co-infections identified were ZIKV+HIV (0.3%, 1\/340), ZIKV+measles (0.3%, 1\/340), ZIKV+parvovirus B19 (0.3%, 1\/340), ZIKV+EBV (0.3%, 1\/340), EBV+parvovirus B19 (0.3%, 1\/340), CMV+parvovirus B19 (0.6%, 2\/340), CMV+syphilis (0.3%, 1\/340), ZIKV+EBV+parvovirus B19 (0.3%, 1\/340) and CMV+EBV+parvovirus B19 (0.9%, 3\/340). Approximately one quarter of patients had no defined cause for their skin rash (25.3%, 86\/340).<\/jats:p><\/jats:sec><jats:sec id=\"sec003\"><jats:title>Conclusions<\/jats:title><jats:p>Despite the benign clinical evolution of most of the diseases diagnosed in this series of cases, syndromic surveillance of diseases such as syphilis and HIV are of utmost importance. Periodic serosurveillance might also aid in evaluating the trends of endemic diseases and eventual outbreaks.<\/jats:p><\/jats:sec>","DOI":"10.1371\/journal.pntd.0010727","type":"journal-article","created":{"date-parts":[[2022,10,13]],"date-time":"2022-10-13T18:00:13Z","timestamp":1665684013000},"page":"e0010727","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":9,"title":["Prevalence of arboviruses and other infectious causes of skin rash in patients treated at a tertiary health unit in the Brazilian Amazon"],"prefix":"10.1371","volume":"16","author":[{"given":"Luiz Henrique","family":"Gon\u00e7alves Maciel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cosmo","family":"Vieira da Rocha Neto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasmin","family":"Ferreira Martins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francielen","family":"de Azevedo Furtado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P\u00e2mela","family":"Cunha Teixeira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maianne Yasmin","family":"Oliveira Dias","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanka Karolinna","family":"Batista Rodrigues","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isa Cristina","family":"Ribeiro Piauilino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9rgio","family":"Damasceno Pinto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aline Cristiane","family":"C\u00f4rte Alencar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o Bosco","family":"de Lima Gimaque","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Paula","family":"Gomes Mour\u00e3o","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcus Vinicius","family":"Guimar\u00e3es Lacerda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e1rcia","family":"da Costa Castilho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6867-4931","authenticated-orcid":true,"given":"Camila","family":"B\u00f4tto-Menezes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,10,13]]},"reference":[{"issue":"2","key":"pntd.0010727.ref001","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.antiviral.2009.10.008","article-title":"Present and Future Arboviral Threaths.","volume":"85","author":"SC Weaver","year":"2010","journal-title":"Antiviral Research"},{"key":"pntd.0010727.ref002","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.actatropica.2018.11.010","article-title":"Molecular epidemiology of dengue, yellow fever, Zika and Chikungunya arboviruses: An update","volume":"190","author":"A Higuera","year":"2019","journal-title":"Acta Trop [Internet]."},{"key":"pntd.0010727.ref003","first-page":"1","article-title":"Emerging arboviruses and public health challenges in Brazil.","volume":"50","author":"TN Lima-Camara","year":"2016","journal-title":"Rev Saude Publica"},{"key":"pntd.0010727.ref004","first-page":"1","article-title":"Emergence of chikungunya and Zika in a municipality endemic to dengue, Santa Luzia, MG, Brazil, 2015\u20132017.","author":"F Liliana","year":"2019","journal-title":"Rev. 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Manual T\u00e9cnico para Diagn\u00f3stico da S\u00edfilis \/ Minist\u00e9rio da Sa\u00fade, Secretaria de Vigil\u00e2ncia em Sa\u00fade, Departamento de Vigil\u00e2ncia, Preven\u00e7\u00e3o e Controle das Doen\u00e7as Sexualmente Transmiss\u00edveis, Aids e Hepatites Virais","year":"2016"},{"key":"pntd.0010727.ref021","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jcv.2017.11.006","article-title":"Real-time RT-PCR for Mayaro virus detection in plasma and urine.","volume":"98","author":"JJ Waggoner","year":"2018","journal-title":"J Clin Virol."},{"issue":"7","key":"pntd.0010727.ref022","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1590\/0074-02760160062","article-title":"Multiplexed reverse transcription real-time polymerase chain reaction for simultaneous detection of Mayaro, Oropouche, and Oropouche-like viruses","volume":"112","author":"FG Naveca","year":"2017","journal-title":"Mem Inst Oswaldo Cruz"},{"issue":"1\u20132","key":"pntd.0010727.ref023","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/j.jviromet.2005.10.006","article-title":"Development of quantitative gene-specific real-time RT-PCR assays for the detection of measles virus in clinical specimens","volume":"132","author":"KB Hummel","year":"2006","journal-title":"J Virol Methods"},{"key":"pntd.0010727.ref024","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.jcv.2017.09.002","article-title":"Clinical, laboratory and virological data from suspected ZIKV patients in an endemic arbovirus area.","volume":"96","author":"TE Colombo","year":"2017","journal-title":"J Clin Virol"},{"issue":"4","key":"pntd.0010727.ref025","first-page":"287","article-title":"Caracter\u00edsticas epidemiol\u00f3gicas da dengue na comunidade S\u00e3o Janu\u00e1rio II na cidade de Campina Grande\u2013PB Epidemiological characteristics of dengue in the community Janu\u00e1rio II in the city of Campina","volume":"92","author":"WD Cavalcante","year":"2011","journal-title":"Brazilian Journal of Pharmacy"},{"issue":"4","key":"pntd.0010727.ref026","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0004636","article-title":"Zika Virus Outbreak in Rio de Janeiro, Brazil: Clinical Characterization, Epidemiological and Virological Aspects.","volume":"10","author":"P Brasil","year":"2016","journal-title":"PLoS Negl Trop Dis."},{"issue":"40","key":"pntd.0010727.ref027","first-page":"1","article-title":"Epidemiological profile of Zika, Dengue and Chikungunya virus infections identified by medical and molecular evaluations in Rondonia, Brazil.","volume":"61","author":"DS Vieira","year":"2019","journal-title":"Rev. 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