{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T15:36:49Z","timestamp":1782488209588,"version":"3.54.5"},"reference-count":32,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T00:00:00Z","timestamp":1667347200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p><jats:italic>Madurella mycetomatis<\/jats:italic> is one of the main causative agents of mycetoma, a debilitating neglected tropical disease. Improved understanding of the genomic diversity of the fungal and bacterial causes of mycetoma is essential to advances in diagnosis and treatment. Here, we describe a high-quality genome assembly of <jats:italic>M<\/jats:italic>. <jats:italic>mycetomatis<\/jats:italic> and results of the whole genome sequence analysis of 26 isolates from Sudan. We demonstrate evidence of at least seven genetically diverse lineages and extreme clonality among isolates within these lineages. We also performed shotgun metagenomic analysis of DNA extracted from mycetoma grains and showed that <jats:italic>M<\/jats:italic>. <jats:italic>mycetomatis<\/jats:italic> reads were detected in all sequenced samples with the average of 11,317 reads (s.d. +\/- 21,269) per sample. In addition, 10 (12%) of the 81 tested grain samples contained bacterial reads including <jats:italic>Streptococcus<\/jats:italic> sp., <jats:italic>Staphylococcus<\/jats:italic> sp. and others.<\/jats:p>","DOI":"10.1371\/journal.pntd.0010787","type":"journal-article","created":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T18:02:35Z","timestamp":1667412155000},"page":"e0010787","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":7,"title":["Genomics and metagenomics of Madurella mycetomatis, a causative agent of black grain mycetoma in Sudan"],"prefix":"10.1371","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1086-8041","authenticated-orcid":true,"given":"Anastasia 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