{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T23:12:06Z","timestamp":1789427526809,"version":"build-2803163510"},"reference-count":85,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2022,10,31]],"date-time":"2022-10-31T00:00:00Z","timestamp":1667174400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81871673,82172300"],"award-info":[{"award-number":["81871673,82172300"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Background<\/jats:title>\n<jats:p>Pyruvate kinase widely exists in many parasites and plays an important role in the energy production for the parasites. Pyruvate kinase might be a potential drug target for killing the parasites. The aim of the present study was to evaluate the biological characteristics and roles of <jats:italic>T<\/jats:italic>. <jats:italic>spiralis<\/jats:italic> pyruvate kinase M (TsPKM) in sugar metabolism, larval molting and development of <jats:italic>T<\/jats:italic>. <jats:italic>spiralis<\/jats:italic>.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methodology\/Principal findings<\/jats:title>\n<jats:p>TsPKM has two functional domains of pyruvate kinase and the tertiary structure of TsPKM is tetramer which has the enzyme active site constituted by 8 amino-acid residues (Arg71, Asn73, Asp110, Phe241, Lys267, Glu269, Asp293 and Thr325). Recombinant TsPKM (rTsPKM) was expressed and purified. The rTsPKM had good immunogenicity. RT-PCR and Western blot showed that TsPKM was transcribed and expressed at various developmental stages in <jats:italic>T<\/jats:italic>. <jats:italic>spiralis<\/jats:italic> lifecycle. Immunofluorescence test showed that TsPKM was principally located in the cuticle, muscle, stichosome, intestine and the intrauterine embryos of female adults. rTsPKM catalyzed the reaction of phosphoenolpyruvate (PEP) and adenosine diphosphate (ADP) to produce pyruvic acid and adenosine triphosphate (ATP). TsPKM played an important role in the metabolism and energy production of <jats:italic>T<\/jats:italic>. <jats:italic>spiralis<\/jats:italic>. After silencing of TsPKM gene by specific dsRNA-TsPKM2, protein expression and enzyme activity of TsPKM decreased by 50.91 and 26.06%, respectively. After treatment with RNAi, natural TsPKM enzyme activity, larval molting, sugar metabolism, growth and development of <jats:italic>T<\/jats:italic>. <jats:italic>spiralis<\/jats:italic> were significantly reduced.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Conclusions<\/jats:title>\n<jats:p>TsPKM participates in the larval molting, sugar metabolism, growth and development of <jats:italic>T<\/jats:italic>. <jats:italic>spiralis<\/jats:italic> and it might be a candidate target of therapeutic drug of trichinellosis.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pntd.0010881","type":"journal-article","created":{"date-parts":[[2022,10,31]],"date-time":"2022-10-31T17:37:18Z","timestamp":1667237838000},"page":"e0010881","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":17,"title":["Characterization of a novel pyruvate kinase from Trichinella spiralis and its participation in sugar metabolism, 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Quan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8726-5498","authenticated-orcid":true,"given":"Jing","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,10,31]]},"reference":[{"key":"pntd.0010881.ref001","doi-asserted-by":"crossref","first-page":"e00205","DOI":"10.1016\/j.parepi.2021.e00205","article-title":"Surveillance of foodborne parasitic diseases in Europe in a One Health approach","volume":"13","author":"J van der Giessen","year":"2021","journal-title":"Parasite Epidemiol Control"},{"key":"pntd.0010881.ref002","doi-asserted-by":"crossref","first-page":"106388","DOI":"10.1016\/j.actatropica.2022.106388","article-title":"Epidemiology of trichinellosis in the People\u2019s Republic of China during 2009\u20132020","volume":"229","author":"XZ 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