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Development of anti-<jats:italic>Trichinella<\/jats:italic>vaccine is needed to control<jats:italic>Trichinella<\/jats:italic>infection in food animals. In this study, two novel<jats:italic>T<\/jats:italic>.<jats:italic>spiralis<\/jats:italic>genes (calreticulin and serine protease 1.1) in combination were used to construct oral DNA vaccines, and their induced protective immunity was evaluated in a murine model.<\/jats:p><\/jats:sec><jats:sec id=\"sec002\"><jats:title>Methodology\/Principal findings<\/jats:title><jats:p>TsCRT+TsSP1.1, TsCRT and TsSP1.1 DNA were transformed into attenuated<jats:italic>Salmonella typhimurium<\/jats:italic>\u0394cyaSL1344. Oral vaccination of mice with TsCRT+TsSP1.1, TsCRT and TsSP1.1 DNA vaccines elicited a gut local mucosal sIgA response and systemic Th1\/Th2 mixed response. Oral vaccination with TsCRT+TsSP1.1 induced obviously higher level of serum specific antibodies, mucosal sIgA and cellular immune response than either of single TsCRT or TsSP1.1 DNA vaccination. Oral vaccination of mice with TsCRT+TsSP1.1 exhibited a 53.4% reduction of enteral adult worms and a 46.05% reduction of muscle larvae, conferred a higher immune protection than either of individual TsCRT (44.28 and 42.46%) or TsSP1.1 DNA vaccine (35.43 and 29.29%) alone. Oral vaccination with TsCRT+TsSP1.1, TsCRT and TsSP1.1 also obviously ameliorated inflammation of intestinal mucosa and skeletal muscles of vaccinated mice after challenge.<\/jats:p><\/jats:sec><jats:sec id=\"sec003\"><jats:title>Conclusions<\/jats:title><jats:p>TsCRT and TsSP1.1 might be regarded the novel potential targets for anti-<jats:italic>Trichinella<\/jats:italic>vaccines. Attenuated<jats:italic>Salmonella<\/jats:italic>-delivered DNA vaccine provided a prospective approach to control<jats:italic>T<\/jats:italic>.<jats:italic>spiralis<\/jats:italic>infection in food animals.<\/jats:p><\/jats:sec>","DOI":"10.1371\/journal.pntd.0010929","type":"journal-article","created":{"date-parts":[[2022,11,29]],"date-time":"2022-11-29T18:28:55Z","timestamp":1669746535000},"page":"e0010929","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":20,"title":["Oral vaccination of mice with attenuated Salmonella encoding Trichinella spiralis calreticulin and serine protease 1.1 confers protective immunity in BALB\/c mice"],"prefix":"10.1371","volume":"16","author":[{"given":"Sheng Jie","family":"Bai","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu Lu","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruo Dan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shao Rong","family":"Long","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8726-5498","authenticated-orcid":true,"given":"Jing","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhong Quan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,11,29]]},"reference":[{"issue":"1\u20132","key":"pntd.0010929.ref001","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.vetpar.2005.05.024","article-title":"The broad spectrum of Trichinella hosts: from cold- to warm-blooded animals","volume":"132","author":"E. 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