{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T14:29:30Z","timestamp":1792160970898,"version":"4.4.0"},"update-to":[{"DOI":"10.1371\/journal.pntd.0010991","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2022,12,30]],"date-time":"2022-12-30T00:00:00Z","timestamp":1672358400000}}],"reference-count":48,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2022,12,16]],"date-time":"2022-12-16T00:00:00Z","timestamp":1671148800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["U19 AI110820"],"award-info":[{"award-number":["U19 AI110820"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Intramural Research Program of the National Institute of Allergy and Infectious Diseases, National Institutes of Health."}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>\n                    <jats:italic>Plasmodium vivax<\/jats:italic>\n                    infections often consist of heterogenous populations of parasites at different developmental stages and with distinct transcriptional profiles, which complicates gene expression analyses. The advent of single cell RNA sequencing (scRNA-seq) enabled disentangling this complexity and has provided robust and stage-specific characterization of\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    gene expression. However, scRNA-seq information is typically derived from the end of each mRNA molecule (usually the 3\u2019-end) and therefore fails to capture the diversity in transcript isoforms documented in bulk RNA-seq data. Here, we describe the sequencing of scRNA-seq libraries using Pacific Biosciences (PacBio) chemistry to characterize full-length\n                    <jats:italic>Plasmodium vivax<\/jats:italic>\n                    transcripts from single cell parasites. Our results show that many\n                    <jats:italic>P<\/jats:italic>\n                    .\n                    <jats:italic>vivax<\/jats:italic>\n                    genes are transcribed into multiple isoforms, primarily through variations in untranslated region (UTR) length or splicing, and that the expression of many isoforms is developmentally regulated. Our findings demonstrate that long read sequencing can be used to characterize mRNA molecules at the single cell level and provides an additional resource to better understand the regulation of gene expression throughout the\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    life cycle.\n                  <\/jats:p>","DOI":"10.1371\/journal.pntd.0010991","type":"journal-article","created":{"date-parts":[[2022,12,16]],"date-time":"2022-12-16T13:32:58Z","timestamp":1671197578000},"page":"e0010991","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":28,"title":["Long read single cell RNA sequencing reveals the isoform diversity of Plasmodium vivax transcripts"],"prefix":"10.1371","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1518-6210","authenticated-orcid":true,"given":"Brittany","family":"Hazzard","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana M.","family":"S\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela C.","family":"Ellis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tales V.","family":"Pascini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuchi","family":"Amin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas E.","family":"Wellems","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2446-9095","authenticated-orcid":true,"given":"David","family":"Serre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,12,16]]},"reference":[{"key":"pntd.0010991.ref001","unstructured":"World Malaria Report 2021. 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