{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T11:30:11Z","timestamp":1767180611020,"version":"build-2238731810"},"update-to":[{"DOI":"10.1371\/journal.pntd.0010994","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2023,1,13]],"date-time":"2023-01-13T00:00:00Z","timestamp":1673568000000}}],"reference-count":19,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2023,1,3]],"date-time":"2023-01-03T00:00:00Z","timestamp":1672704000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006378","name":"Universitas Indonesia","doi-asserted-by":"crossref","award":["Hibah TADOK 2019 (476\/SK\/R\/UI\/2019)"],"award-info":[{"award-number":["Hibah TADOK 2019 (476\/SK\/R\/UI\/2019)"]}],"id":[{"id":"10.13039\/501100006378","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Adipose derived stromal vascular fraction (SVF) contains a heterogeneous population of mononuclear cells, progenitor cells and about 1\u201310% are mesenchymal stromal cells. These cells are an ideal candidate for regenerative medicine for peripheral neuropathy. Leprosy is a disabling disorder with neuropathy, usually with consequences of permanent disability of the extremities. We conducted a preliminary study to evaluate the cell yield, its characteristics and clinical outcomes after SVF injections in four leprosy patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Four post leprosy patients were recruited and evaluated for sensory testing (warm detection, cold detection, vibration, pain and sensation) on the ulnar area of the hand. Liposuction was done and adipose tissue was processed into SVF with a closed system and injected to the ulnar area of the hand at the dorsal and palmar side. Evaluation of sensory testing was done after 3 days, 1 week, 1 month and 3 months following SVF injection. SVF was also characterized using flow cytometry, cell counting, sterility and presence of mycobacteria.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The results showed that leprosy patients had a low count of mesenchymal cells and a high amount of CD34\/CD45 positive cells. One patient was positive for mycobacteria from his adipose tissue and SVF. Sensory examination after SVF injection showed an improvement in temperature and pain sensation in the palmar and superficial branch. Meanwhile, touch sensation improved on the dorsal branch, and there was no improvement for vibration in all patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec004\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The results showed that SVF had a potential to improve sensory loss in leprosy patients.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pntd.0010994","type":"journal-article","created":{"date-parts":[[2023,1,3]],"date-time":"2023-01-03T14:38:40Z","timestamp":1672756720000},"page":"e0010994","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Effect of adipose derived stromal vascular fraction on leprosy neuropathy: A Preliminary report"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5167-5399","authenticated-orcid":true,"given":"Sondang P.","family":"Sirait","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kusmarinah","family":"Bramono","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sri Linuwih","family":"Menaldi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeanne Adiwinata","family":"Pawitan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wresti","family":"Indriatmi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tiara","family":"Aninditha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2023,1,3]]},"reference":[{"key":"pntd.0010994.ref001","doi-asserted-by":"crossref","first-page":"255","DOI":"10.2147\/JPR.S63361","article-title":"A preliminary report on stem cell therapy for neuropathic pain in humans","volume":"7","author":"ER Vickers","year":"2014","journal-title":"Journal of Pain Research"},{"key":"pntd.0010994.ref002","doi-asserted-by":"crossref","first-page":"2175","DOI":"10.1136\/annrheumdis-2014-205681","article-title":"Safety, tolerability and potential efficacy of injection of autologous adipose-derived stromal vascular fraction in the fingers of patients with systemic sclerosis: an open-label phase I trial","volume":"74","author":"B Granel","year":"2015","journal-title":"Ann Rheum Dis"},{"issue":"6","key":"pntd.0010994.ref003","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1016\/j.jcyt.2013.02.006","article-title":"Stromal cells from the adipose tissue-derived stromal vascular fraction and culture expanded adipose tissue-derived stromal.","volume":"15","author":"P Bourin","year":"2013","journal-title":"Cytotherapy"},{"issue":"1","key":"pntd.0010994.ref004","doi-asserted-by":"crossref","first-page":"107","DOI":"10.15283\/ijsc18101","article-title":"Possibility of Injecting Adipose-Derived Stromal Vascular Fraction Cells to Accelerate Microcirculation in Ischemic Diabetic Feet: A Pilot Study.","volume":"12","author":"KC Moon","year":"2019","journal-title":"International Journal of Stem Cells"},{"key":"pntd.0010994.ref005","article-title":"Disability in people affected by leprosy: the role of impairment, activity, social participation, stigma and discrimination.","author":"WH van Brakel","year":"2012","journal-title":"Global Health Action."},{"issue":"11","key":"pntd.0010994.ref006","doi-asserted-by":"crossref","first-page":"e328","DOI":"10.1371\/journal.pntd.0000328","article-title":"Enumeration of Mycobacterium leprae Using Real-Time PCR.","volume":"2","author":"RW Truman","year":"2008","journal-title":"PLoS Negl Trop Dis"},{"key":"pntd.0010994.ref007","doi-asserted-by":"crossref","first-page":"14","DOI":"10.47276\/lr.76.1.14","article-title":"The INFIR cohort study: investigating prediction, detection and pathogenesis of neuropathy and reactions in leprosy. 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Catalog No. 341071. CD45\/CD34. Monoclonal Antibodies Detecting Human Antigens. Retrieved from: https:\/\/www.bd.com\/resource.aspx?idx=1116(n.d.). 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