{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T00:34:25Z","timestamp":1787790865448,"version":"build-2784847793"},"reference-count":63,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2023,1,6]],"date-time":"2023-01-06T00:00:00Z","timestamp":1672963200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministery of Defence","award":["V1802"],"award-info":[{"award-number":["V1802"]}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Background<\/jats:title>\n<jats:p><jats:italic>Burkholderia mallei<\/jats:italic> and <jats:italic>Burkholderia pseudomallei<\/jats:italic> are both potential biological threat agents. Melioidosis caused by <jats:italic>B<\/jats:italic>. <jats:italic>pseudomallei<\/jats:italic> is endemic in Southeast Asia and Northern Australia, while glanders caused by <jats:italic>B<\/jats:italic>. <jats:italic>mallei<\/jats:italic> infections are rare. Here we studied the proteomes of different <jats:italic>B<\/jats:italic>. <jats:italic>mallei<\/jats:italic> and <jats:italic>B<\/jats:italic>. <jats:italic>pseudomallei<\/jats:italic> isolates to determine species specific characteristics.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methods<\/jats:title>\n<jats:p>The expressed proteins of 5 <jats:italic>B<\/jats:italic>. <jats:italic>mallei<\/jats:italic> and 6 <jats:italic>B<\/jats:italic>. <jats:italic>pseudomallei<\/jats:italic> strains were characterized using liquid chromatography high-resolution tandem mass spectrometry (LC-HRMS\/MS). Subsequently, expression of potential resistance and virulence related characteristics were analyzed and compared.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Results<\/jats:title>\n<jats:p>Proteome analysis can be used for the identification of <jats:italic>B<\/jats:italic>. <jats:italic>mallei<\/jats:italic> and <jats:italic>B<\/jats:italic>. <jats:italic>pseudomallei<\/jats:italic>. Both species were identified based on &gt;60 discriminative peptides. Expression of proteins potentially involved in antimicrobial resistance, AmrAB\u2013OprA, BpeAB\u2013OprB, BpeEF\u2013OprC, PenA as well as several other efflux pump related proteins and putative \u03b2-lactamases was demonstrated. Despite, the fact that efflux pump BpeAB\u2013OprB was expressed in all isolates, no clear correlation with an antimicrobial phenotype and the efflux-pump could be established. Also consistent with the phenotypes, no amino acid mutations in PenA known to result in \u03b2-lactam resistance could be identified.<\/jats:p>\n<jats:p>In all studied isolates, the expression of virulence (related) factors Capsule-1 and T2SS was demonstrated. The expression of T6SS-1 was demonstrated in all 6 <jats:italic>B<\/jats:italic>. <jats:italic>pseudomallei<\/jats:italic> isolates and in 2 of the 5 <jats:italic>B<\/jats:italic>. <jats:italic>mallei<\/jats:italic> isolates. In all, except one <jats:italic>B<\/jats:italic>. <jats:italic>pseudomallei<\/jats:italic> isolate, poly-beta-1,6 N-acetyl-D-glucosamine export porin (Pga), important for biofilm formation, was detected, which were absent in the proteomes of <jats:italic>B<\/jats:italic>. <jats:italic>mallei<\/jats:italic>.<\/jats:p>\n<jats:p>Siderophores, iron binding proteins, malleobactin and malleilactone are possibly expressed in both species under standard laboratory growth conditions. Expression of multiple proteins from both the malleobactin and malleilactone polyketide synthase (PKS) and non-ribosomal peptide synthetase (NRPS) clusters was demonstrated in both species. All <jats:italic>B<\/jats:italic>. <jats:italic>pseudomallei<\/jats:italic> expressed at least seven of the nine proteins of the bactobolin synthase cluster (bactobolin, is a ribosome targeting antibiotic), while only in one <jats:italic>B<\/jats:italic>. <jats:italic>mallei<\/jats:italic> isolate expression of two proteins of this synthase cluster was identified.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec004\">\n<jats:title>Conclusions<\/jats:title>\n<jats:p>Analyzing the expressed proteomes revealed differences between <jats:italic>B<\/jats:italic>. <jats:italic>mallei<\/jats:italic> and <jats:italic>B<\/jats:italic>. <jats:italic>pseudomallei<\/jats:italic> but also between isolates from the same species. Proteome analysis can be used not only to identify <jats:italic>B<\/jats:italic>. <jats:italic>mallei<\/jats:italic> and <jats:italic>B<\/jats:italic>. <jats:italic>pseudomallei<\/jats:italic> but also to characterize the presence of important factors that putatively contribute to the pathogenesis of <jats:italic>B<\/jats:italic>. <jats:italic>mallei<\/jats:italic> and <jats:italic>B<\/jats:italic>. <jats:italic>pseudomallei<\/jats:italic>.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pntd.0011006","type":"journal-article","created":{"date-parts":[[2023,1,6]],"date-time":"2023-01-06T18:28:17Z","timestamp":1673029697000},"page":"e0011006","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":6,"title":["Expression of virulence and antimicrobial related proteins in Burkholderia mallei and Burkholderia pseudomallei"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0376-8563","authenticated-orcid":true,"given":"Armand","family":"Paauw","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Holger C.","family":"Scholz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roos H.","family":"Mars-Groenendijk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lennard J. M.","family":"Dekker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theo M.","family":"Luider","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1694-8421","authenticated-orcid":true,"given":"Hans C.","family":"van Leeuwen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,1,6]]},"reference":[{"issue":"1","key":"pntd.0011006.ref001","doi-asserted-by":"crossref","first-page":"38","DOI":"10.3923\/jbs.2014.38.51","article-title":"Glanders-a re-emerging zoonotic disease: a review","volume":"14","author":"AK Verma","year":"2014","journal-title":"Journal of Biological Sciences"},{"issue":"32","key":"pntd.0011006.ref002","doi-asserted-by":"crossref","first-page":"13192","DOI":"10.1021\/ja3052156","article-title":"Malleilactone, a polyketide synthase-derived virulence factor encoded by the cryptic secondary metabolome of Burkholderia pseudomallei group pathogens","volume":"134","author":"JB Biggins","year":"2012","journal-title":"J Am Chem Soc"},{"issue":"1","key":"pntd.0011006.ref003","doi-asserted-by":"crossref","first-page":"):9167,018","DOI":"10.1038\/s41598-018-27356-7","article-title":"Effect of temperature on Burkholderia pseudomallei growth, proteomic changes, motility and resistance to stress environments.","volume":"8","author":"S Paksanont","year":"2018","journal-title":"Sci Rep."},{"issue":"12","key":"pntd.0011006.ref004","doi-asserted-by":"crossref","first-page":"6032","DOI":"10.1128\/JCM.43.12.6032-6034.2005","article-title":"Recurrent melioidosis in patients in northeast Thailand is frequently due to reinfection rather than relapse","volume":"43","author":"B Maharjan","year":"2005","journal-title":"J Clin Microbiol"},{"issue":"2","key":"pntd.0011006.ref005","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1128\/CMR.18.2.383-416.2005","article-title":"Melioidosis: epidemiology, pathophysiology, and management","volume":"18","author":"AC Cheng","year":"2005","journal-title":"Clin Microbiol Rev"},{"issue":"1","key":"pntd.0011006.ref006","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1017\/S0022172400005234","article-title":"An Account of a Glanders-like Disease occurring in Rangoon","volume":"13","author":"A. Whitmore","year":"1913","journal-title":"J Hyg (Lond)"},{"key":"pntd.0011006.ref007","doi-asserted-by":"crossref","first-page":"16263","DOI":"10.1038\/nmicrobiol.2016.263","article-title":"Global and regional dissemination and evolution of Burkholderia pseudomallei","volume":"2","author":"C Chewapreecha","year":"2017","journal-title":"Nat Microbiol"},{"key":"pntd.0011006.ref008","doi-asserted-by":"crossref","first-page":"15008","DOI":"10.1038\/nmicrobiol.2015.8","article-title":"Predicted global distribution of Burkholderia pseudomallei and burden of melioidosis.","volume":"1","author":"D Limmathurotsakul","year":"2016","journal-title":"Nat Microbiol."},{"issue":"6","key":"pntd.0011006.ref009","doi-asserted-by":"crossref","first-page":"1295","DOI":"10.3201\/eid2606.190975","article-title":"Melioidosis in a resident of Texas with no recent travel history, United States","volume":"26","author":"CM Cossaboom","year":"2020","journal-title":"Emerg Infect Dis"},{"issue":"6","key":"pntd.0011006.ref010","doi-asserted-by":"crossref","first-page":"1705","DOI":"10.3201\/eid2706.200154","article-title":"Melioidosis in Children, Brazil, 1989\u20132019","volume":"27","author":"RXR Lima","year":"2021","journal-title":"Emerg Infect Dis"},{"issue":"6","key":"pntd.0011006.ref011","doi-asserted-by":"crossref","first-page":"981","DOI":"10.3201\/eid1906.120717","article-title":"Human melioidosis, Malawi, 2011","volume":"19","author":"T Katangwe","year":"2013","journal-title":"Emerg Infect Dis"},{"key":"pntd.0011006.ref012","doi-asserted-by":"crossref","first-page":"101461","DOI":"10.1016\/j.tmaid.2019.07.017","article-title":"Melioidosis in travelers: An analysis of Dutch melioidosis registry data 1985\u20132018.","volume":"29","author":"E Birnie","year":"2019","journal-title":"Travel Med Infect Dis"},{"issue":"11","key":"pntd.0011006.ref013","doi-asserted-by":"crossref","first-page":"e0241306","DOI":"10.1371\/journal.pone.0241306","article-title":"Prediction of Burkholderia pseudomallei DsbA substrates identifies potential virulence factors and vaccine targets","volume":"15","author":"B Vezina","year":"2020","journal-title":"PLoS One"},{"issue":"8","key":"pntd.0011006.ref014","doi-asserted-by":"crossref","first-page":"892","DOI":"10.1016\/S1473-3099(19)30157-4","article-title":"Global burden of melioidosis in 2015: a systematic review and data synthesis","volume":"19","author":"E Birnie","year":"2019","journal-title":"Lancet Infect Dis"},{"issue":"1","key":"pntd.0011006.ref015","doi-asserted-by":"crossref","first-page":"151376","DOI":"10.1016\/j.ijmm.2019.151376","article-title":"Untargeted accurate identification of highly pathogenic bacteria directly from blood culture flasks","volume":"310","author":"EM Berendsen","year":"2020","journal-title":"Int J Med Microbiol"},{"issue":"4","key":"pntd.0011006.ref016","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/j.micinf.2011.11.011","article-title":"Identification of differentially expressed proteins from Burkholderia pseudomallei isolated during primary and relapsing melioidosis","volume":"14","author":"B Velapatino","year":"2012","journal-title":"Microbes Infect"},{"issue":"8","key":"pntd.0011006.ref017","doi-asserted-by":"crossref","first-page":"3214","DOI":"10.1128\/IAI.01739-14","article-title":"Proteomic analysis of the Burkholderia pseudomallei type II secretome reveals hydrolytic enzymes, novel proteins, and the deubiquitinase TssM","volume":"82","author":"MN Burtnick","year":"2014","journal-title":"Infect Immun"},{"issue":"1","key":"pntd.0011006.ref018","doi-asserted-by":"crossref","first-page":"9015","DOI":"10.1038\/s41598-017-09373-0","article-title":"Host-Adaptation of Burkholderia pseudomallei alters metabolism and virulence: a global proteome analysis.","volume":"7","author":"V Mariappan","year":"2017","journal-title":"Sci Rep"},{"issue":"5","key":"pntd.0011006.ref019","doi-asserted-by":"crossref","first-page":"e0127398","DOI":"10.1371\/journal.pone.0127398","article-title":"Altered proteome of Burkholderia pseudomallei colony variants induced by exposure to human lung epithelial cells","volume":"10","author":"AR Al-Maleki","year":"2015","journal-title":"PLoS One"},{"issue":"5","key":"pntd.0011006.ref020","doi-asserted-by":"crossref","first-page":"2417","DOI":"10.1021\/pr1012398","article-title":"Outer membrane proteome of Burkholderia pseudomallei and Burkholderia mallei from diverse growth conditions","volume":"10","author":"MA Schell","year":"2011","journal-title":"J Proteome Res"},{"issue":"5","key":"pntd.0011006.ref021","doi-asserted-by":"crossref","first-page":"2068","DOI":"10.1128\/JCM.41.5.2068-2079.2003","article-title":"Multilocus sequence typing and evolutionary relationships among the causative agents of melioidosis and glanders, Burkholderia pseudomallei and Burkholderia mallei","volume":"41","author":"D Godoy","year":"2003","journal-title":"J Clin Microbiol"},{"key":"pntd.0011006.ref022","doi-asserted-by":"crossref","first-page":"124","DOI":"10.12688\/wellcomeopenres.14826.1","article-title":"Open-access bacterial population genomics: BIGSdb software, the PubMLST.org website and their applications.","volume":"3","author":"KA Jolley","year":"2018","journal-title":"Wellcome Open Res."},{"issue":"2","key":"pntd.0011006.ref023","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/S0924-8579(00)00334-4","article-title":"Antibiotic susceptibility of Burkholderia pseudomallei from tropical northern Australia and implications for therapy of melioidosis.","volume":"17","author":"AW Jenney","year":"2001","journal-title":"Int J Antimicrob Agents."},{"issue":"5","key":"pntd.0011006.ref024","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1016\/S0924-8579(02)00016-X","article-title":"A comparison of antibiotic susceptibility testing methods for cotrimoxazole with Burkholderia pseudomallei.","volume":"19","author":"P Piliouras","year":"2002","journal-title":"Int J Antimicrob Agents."},{"key":"pntd.0011006.ref025","doi-asserted-by":"crossref","first-page":"121845","DOI":"10.1155\/2013\/121845","article-title":"The in vitro antibiotic susceptibility of Malaysian isolates of Burkholderia pseudomallei","volume":"2013","author":"N Ahmad","year":"2013","journal-title":"Int J Microbiol"},{"issue":"4","key":"pntd.0011006.ref026","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1016\/j.ijantimicag.2014.06.003","article-title":"Trimethoprim\/sulfamethoxazole resistance in Burkholderia pseudomallei.","volume":"44","author":"DA Dance","year":"2014","journal-title":"Int J Antimicrob Agents."},{"key":"pntd.0011006.ref027","unstructured":"Clinical and Laboratory Standard Institute. M45, 3rd edition. Methods for antimicrobial dilution and disk susceptibility testing of infrequently isolated or fastidious bacteria, Wayne, Pa, USA."},{"key":"pntd.0011006.ref028","unstructured":"Clinical and Laboratory Standard Institute. M100, 30th edition. Performance standards for antimicrobial susceptibility testing clinical and laboratory standards, Wayne, Pa, USA."},{"issue":"5","key":"pntd.0011006.ref029","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1038\/nmeth.1322","article-title":"Universal sample preparation method for proteome analysis.","volume":"6","author":"JR Wisniewski","year":"2009","journal-title":"Nat Methods."},{"issue":"D1","key":"pntd.0011006.ref030","doi-asserted-by":"crossref","first-page":"D442","DOI":"10.1093\/nar\/gky1106","article-title":"The PRIDE database and related tools and resources in 2019: improving support for quantification data.","volume":"47","author":"Y Perez-Riverol","year":"2019","journal-title":"Nucleic Acids Res"},{"issue":"D1","key":"pntd.0011006.ref031","first-page":"D1145","article-title":"The ProteomeXchange consortium in 2020: enabling \u2019big data\u2019 approaches in proteomics.","volume":"48","author":"EW Deutsch","year":"2020","journal-title":"Nucleic Acids Res"},{"issue":"4","key":"pntd.0011006.ref032","doi-asserted-by":"crossref","first-page":"a025387","DOI":"10.1101\/cshperspect.a025387","article-title":"Tetracycline Antibiotics and Resistance.","volume":"6","author":"TH Grossman","year":"2016","journal-title":"Cold Spring Harb Perspect Med."},{"issue":"D1","key":"pntd.0011006.ref033","doi-asserted-by":"crossref","first-page":"D687","DOI":"10.1093\/nar\/gky1080","article-title":"VFDB 2019: a comparative pathogenomic platform with an interactive web interface.","volume":"47","author":"B Liu","year":"2019","journal-title":"Nucleic Acids Res"},{"issue":"4","key":"pntd.0011006.ref034","doi-asserted-by":"crossref","first-page":"716","DOI":"10.1021\/ol902751x","article-title":"Quorum-sensing-regulated bactobolin production by Burkholderia thailandensis E264","volume":"12","author":"MR Seyedsayamdost","year":"2010","journal-title":"Org Lett"},{"issue":"14","key":"pntd.0011006.ref035","doi-asserted-by":"crossref","DOI":"10.1128\/JB.00008-18","article-title":"Malleilactone is a Burkholderia pseudomallei virulence factor regulated by antibiotics and quorum sensing","volume":"200","author":"JR Klaus","year":"2018","journal-title":"J Bacteriol"},{"key":"pntd.0011006.ref036","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.micpath.2017.03.015","article-title":"A proteasome inhibitor produced by Burkholderia pseudomallei modulates intracellular growth","volume":"107","author":"S Wagley","year":"2017","journal-title":"Microb Pathog"},{"issue":"40","key":"pntd.0011006.ref037","doi-asserted-by":"crossref","first-page":"12102","DOI":"10.1021\/ja075492v","article-title":"Natural products from isnA-containing biosynthetic gene clusters recovered from the genomes of cultured and uncultured bacteria","volume":"129","author":"SF Brady","year":"2007","journal-title":"J Am Chem Soc"},{"issue":"4","key":"pntd.0011006.ref038","doi-asserted-by":"crossref","first-page":"1551","DOI":"10.1128\/JB.188.4.1551-1566.2006","article-title":"Genetic and transcriptional analysis of the siderophore malleobactin biosynthesis and transport genes in the human pathogen Burkholderia pseudomallei K96243","volume":"188","author":"AF Alice","year":"2006","journal-title":"J Bacteriol"},{"issue":"26","key":"pntd.0011006.ref039","doi-asserted-by":"crossref","first-page":"9484","DOI":"10.1021\/ja504617n","article-title":"The chemical arsenal of Burkholderia pseudomallei is essential for pathogenicity","volume":"136","author":"JB Biggins","year":"2014","journal-title":"J Am Chem Soc"},{"issue":"7","key":"pntd.0011006.ref040","doi-asserted-by":"crossref","first-page":"e0270499","DOI":"10.1371\/journal.pone.0270499","article-title":"Genetic diversity and spatial distribution of Burkholderia mallei by core genome-based multilocus sequence typing analysis.","volume":"17","author":"S Appelt","year":"2022","journal-title":"PLoS One."},{"key":"pntd.0011006.ref041","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/j.jgar.2017.04.012","article-title":"Epidemiological cut-off value of clinical isolates of Burkholderia pseudomallei from Northern Queensland to meropenem, ceftazidime, trimethoprim\/sulfamethoxazole and doxycycline by the microbroth dilution method.","volume":"10","author":"S Maloney","year":"2017","journal-title":"J Glob Antimicrob Resist."},{"issue":"2","key":"pntd.0011006.ref042","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.ijantimicag.2014.04.012","article-title":"Current antimicrobial susceptibility of first-episode melioidosis Burkholderia pseudomallei isolates from the Northern Territory, Australia.","volume":"44","author":"A Crowe","year":"2014","journal-title":"Int J Antimicrob Agents."},{"key":"pntd.0011006.ref043","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.jgar.2019.01.024","article-title":"Antimicrobial susceptibility of Burkholderia pseudomallei isolates in Northern Vietnam.","volume":"18","author":"PH Nhung","year":"2019","journal-title":"J Glob Antimicrob Resist"},{"issue":"1","key":"pntd.0011006.ref044","doi-asserted-by":"crossref","first-page":"299,017","DOI":"10.1186\/s13104-017-2626-5","article-title":"Antimicrobial susceptibility pattern of clinical isolates of Burkholderia pseudomallei in Bangladesh.","volume":"10","author":"S Dutta","year":"2017","journal-title":"BMC Res Notes."},{"issue":"6","key":"pntd.0011006.ref045","doi-asserted-by":"crossref","first-page":"e00203","DOI":"10.1128\/JCM.00203-19","article-title":"Understanding and Addressing CLSI Breakpoint Revisions: a Primer for Clinical Laboratories","volume":"57","author":"RM Humphries","year":"2019","journal-title":"J Clin Microbiol"},{"issue":"6","key":"pntd.0011006.ref046","doi-asserted-by":"crossref","DOI":"10.1128\/AAC.00010-17","article-title":"Antibiotic resistance markers in Burkholderia pseudomallei strain Bp1651 identified by genome sequence analysis","volume":"61","author":"JV Bugrysheva","year":"2017","journal-title":"Antimicrob Agents Chemother"},{"key":"pntd.0011006.ref047","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.drup.2016.07.003","article-title":"Antibiotic resistance in Burkholderia species","volume":"28","author":"KA Rhodes","year":"2016","journal-title":"Drug Resist Updat"},{"issue":"5","key":"pntd.0011006.ref048","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.01357-17","article-title":"Mechanisms of resistance to folate pathway inhibitors in Burkholderia pseudomallei: Deviation from the norm.","volume":"8","author":"NL Podnecky","year":"2017","journal-title":"MBio"},{"issue":"6","key":"pntd.0011006.ref049","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1016\/j.ijantimicag.2011.02.001","article-title":"Comparative in vitro susceptibility of Burkholderia pseudomallei to doripenem, ertapenem, tigecycline and moxifloxacin.","volume":"37","author":"P Harris","year":"2011","journal-title":"Int J Antimicrob Agents."},{"key":"pntd.0011006.ref050","doi-asserted-by":"crossref","first-page":"305","DOI":"10.3389\/fmicb.2015.00305","article-title":"Efflux pump-mediated drug resistance in Burkholderia.","volume":"6","author":"NL Podnecky","year":"2015","journal-title":"Front Microbiol."},{"key":"pntd.0011006.ref051","first-page":"109583","article-title":"The identification and differentiation between Burkholderia mallei and Burkholderia pseudomallei using one gene pyrosequencing","volume":"2014","author":"DH Gilling","year":"2014","journal-title":"Int Sch Res Notices"},{"issue":"10","key":"pntd.0011006.ref052","doi-asserted-by":"crossref","first-page":"3670","DOI":"10.1128\/JB.01920-07","article-title":"Roles of pgaABCD genes in synthesis, modification, and export of the Escherichia coli biofilm adhesin poly-beta-1,6-N-acetyl-D-glucosamine.","volume":"190","author":"Y Itoh","year":"2008","journal-title":"J Bacteriol"},{"issue":"6","key":"pntd.0011006.ref053","doi-asserted-by":"crossref","first-page":"2501","DOI":"10.1021\/acs.jproteome.9b00714","article-title":"TaxIt: An iterative computational pipeline for untargeted strain-level identification using MS\/MS spectra from pathogenic single-organism samples","volume":"19","author":"M Kuhring","year":"2020","journal-title":"J Proteome Res"},{"key":"pntd.0011006.ref054","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.2217\/fmb-2017-0050","article-title":"Identification of microorganisms grown in blood culture flasks using liquid chromatography-tandem mass spectrometry","volume":"12","author":"EM Berendsen","year":"2017","journal-title":"Future Microbiol"},{"issue":"5","key":"pntd.0011006.ref055","doi-asserted-by":"crossref","first-page":"674","DOI":"10.3390\/antibiotics11050674","article-title":"The class A \u03b2-Lactamase produced by Burkholderia species compromises the potency of Tebipenem against a panel of isolates from the United States.","volume":"11","author":"SA Becka","year":"2022","journal-title":"Antibiotics (Basel)."},{"issue":"2","key":"pntd.0011006.ref056","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1128\/AAC.44.2.362-367.2000","article-title":"High-level expression of chromosomally encoded SHV-1 beta-lactamase and an outer membrane protein change confer resistance to ceftazidime and piperacillin-tazobactam in a clinical isolate of Klebsiella pneumoniae","volume":"44","author":"LB Rice","year":"2000","journal-title":"Antimicrob Agents Chemother"},{"issue":"9","key":"pntd.0011006.ref057","doi-asserted-by":"crossref","first-page":"4381","DOI":"10.1128\/AAC.00660-13","article-title":"The BpeEF-OprC efflux pump is responsible for widespread trimethoprim resistance in clinical and environmental Burkholderia pseudomallei isolates","volume":"57","author":"NL Podnecky","year":"2013","journal-title":"Antimicrob Agents Chemother"},{"issue":"6","key":"pntd.0011006.ref058","first-page":"e00268","article-title":"Loss of methyltransferase function and increased efflux activity deads to Doxycycline resistance in Burkholderia pseudomallei","volume":"61","author":"JR Webb","year":"2017","journal-title":"Antimicrob Agents Chemother"},{"issue":"3","key":"pntd.0011006.ref059","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1111\/j.1574-695X.2002.tb00629.x","article-title":"A possible pitfall in the identification of Burkholderia mallei using molecular identification systems based on the sequence of the flagellin fliC gene","volume":"34","author":"LD Sprague","year":"2002","journal-title":"FEMS Immunol Med Microbiol"},{"key":"pntd.0011006.ref060","doi-asserted-by":"crossref","first-page":"27475","DOI":"10.1038\/srep27475","article-title":"Detection of Burkholderia pseudomallei toxin-mediated inhibition of protein synthesis using a Caenorhabditis elegans ugt-29 biosensor.","volume":"6","author":"RR Wong","year":"2016","journal-title":"Sci Rep"},{"issue":"1","key":"pntd.0011006.ref061","doi-asserted-by":"crossref","first-page":"8320,020","DOI":"10.1038\/s41598-020-64914-4","article-title":"In vitro passage alters virulence, immune activation and proteomic profiles of Burkholderia pseudomallei.","volume":"10","author":"T Duangurai","year":"2020","journal-title":"Sci Rep."},{"issue":"12","key":"pntd.0011006.ref062","doi-asserted-by":"crossref","first-page":"1487","DOI":"10.1586\/14787210.2014.970634","article-title":"Melioidosis: molecular aspects of pathogenesis.","volume":"12","author":"JK Stone","year":"2014","journal-title":"Expert Rev Anti Infect Ther."},{"key":"pntd.0011006.ref063","doi-asserted-by":"crossref","first-page":"3039106","DOI":"10.1155\/2018\/3039106","article-title":"Burkholderia pseudomallei adaptation for survival in stressful conditions","volume":"2018","author":"T Duangurai","year":"2018","journal-title":"Biomed Res Int"}],"container-title":["PLOS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0011006","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,6]],"date-time":"2023-01-06T18:28:55Z","timestamp":1673029735000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0011006"}},"subtitle":[],"editor":[{"given":"Bradley R.","family":"Borlee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2023,1,6]]},"references-count":63,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2023,1,6]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0011006","relation":{},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1,6]]}}}