{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T17:49:52Z","timestamp":1793728192088,"version":"4.6.1"},"update-to":[{"DOI":"10.1371\/journal.pntd.0011038","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2023,5,11]],"date-time":"2023-05-11T00:00:00Z","timestamp":1683763200000}}],"reference-count":50,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2023,5,1]],"date-time":"2023-05-01T00:00:00Z","timestamp":1682899200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University of Sydney Research Training Program (RTP) Scholarship, Australian Government"},{"name":"ASEAN-EU Cooperation in Science, Technology and Innovation, Southeast Asia - Europe Joint Funding Scheme 2019","award":["SEAEUROPEJFS19IN-053"],"award-info":[{"award-number":["SEAEUROPEJFS19IN-053"]}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Background<\/jats:title>\n<jats:p><jats:italic>Angiostrongylus cantonensis<\/jats:italic> (rat lungworm) is recognised as the leading cause of human eosinophilic meningitis, a serious condition observed when nematode larvae migrate through the CNS. Canine Neural Angiostrongyliasis (CNA) is the analogous disease in dogs. Both humans and dogs are accidental hosts, and a rapid diagnosis is warranted. A highly sensitive PCR based assay is available but often not readily accessible in many jurisdictions. An alternative DNA amplification assay that would further improve accessibility is needed. This study aimed to assess the diagnostic utility of a newly designed LAMP assay to detect DNA of globally distributed and invasive <jats:italic>A<\/jats:italic>. <jats:italic>cantonensis<\/jats:italic> and <jats:italic>Angiostrongylus mackerrasae<\/jats:italic>, the other neurotropic <jats:italic>Angiostrongylus<\/jats:italic> species, which is native to Australia.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methodology\/Principal findings<\/jats:title>\n<jats:p>Cerebrospinal fluid (CSF) from dogs with a presumptive diagnosis of <jats:italic>A<\/jats:italic>. <jats:italic>cantonensis<\/jats:italic> infection (2020\u20132022) were received for confirmatory laboratory testing and processed for DNA isolation and ultrasensitive <jats:italic>Angiostrongylus<\/jats:italic> qPCR targeting AcanR3390. A newly designed LAMP assay targeting the same gene target was directly compared to the reference ultrasensitive qPCR in a diagnostic laboratory setting to determine the presence of <jats:italic>A<\/jats:italic>. <jats:italic>cantonensis<\/jats:italic> DNA to diagnose CNA. The LAMP assay (Angie-LAMP) allowed the sensitive detection of <jats:italic>A<\/jats:italic>. <jats:italic>cantonensis<\/jats:italic> DNA from archived DNA specimens (Kappa = 0.81, 95%CI 0.69\u20130.92; <jats:italic>n<\/jats:italic> = 93) and rapid single-step lysis of archived CSF samples (Kappa = 0.77, 95%CI 0.59\u20130.94; <jats:italic>n<\/jats:italic> = 52). Only <jats:italic>A<\/jats:italic>. <jats:italic>cantonensis<\/jats:italic> DNA was detected in canine CSF samples, and co-infection with <jats:italic>A<\/jats:italic>. <jats:italic>mackerrasae<\/jats:italic> using amplicon deep sequencing (ITS-2 rDNA) was not demonstrated. Both SYD.1 and AC13 haplotypes were detected using sequencing of partial <jats:italic>cox<\/jats:italic>1.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Conclusions\/Significance<\/jats:title>\n<jats:p>The Angie-LAMP assay is a useful molecular tool for detecting <jats:italic>Angiostrongylus<\/jats:italic> DNA in canine CSF and performs comparably to a laboratory <jats:italic>Angiostrongylus<\/jats:italic> qPCR. Adaptation of single-step sample lysis improved potential applicability for diagnosis of angiostrongyliasis in a clinical setting for dogs and by extension, to humans.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pntd.0011038","type":"journal-article","created":{"date-parts":[[2023,5,1]],"date-time":"2023-05-01T17:29:52Z","timestamp":1682962192000},"page":"e0011038","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":15,"title":["Angie-LAMP for diagnosis of human eosinophilic meningitis using dog as proxy: A LAMP assay for Angiostrongylus cantonensis DNA in cerebrospinal fluid"],"prefix":"10.1371","volume":"17","author":[{"given":"Vojtech","family":"Bal\u00e1\u017e","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phoebe","family":"Rivory","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas","family":"Hayward","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan","family":"Jaensch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Malik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rogan","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Modr\u00fd","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1459-9117","authenticated-orcid":true,"given":"Jan","family":"\u0160lapeta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,5,1]]},"reference":[{"issue":"7","key":"pntd.0011038.ref001","doi-asserted-by":"crossref","first-page":"1234","DOI":"10.3201\/eid2107.141980","article-title":"Geographic range expansion for rat lungworm in North America","volume":"21","author":"EM York","year":"2015","journal-title":"Emerging Infect Dis"},{"issue":"33","key":"pntd.0011038.ref002","doi-asserted-by":"crossref","first-page":"1900489","DOI":"10.2807\/1560-7917.ES.2019.24.33.1900489","article-title":"Angiostrongylus cantonensis in North African hedgehogs as vertebrate hosts, Mallorca, Spain, October 2018","volume":"24","author":"C Paredes-Esquivel","year":"2019","journal-title":"Euro Surveill"},{"issue":"6","key":"pntd.0011038.ref003","doi-asserted-by":"crossref","first-page":"1163","DOI":"10.3201\/eid2806.212344","article-title":"Angiostrongylus cantonensis nematode invasion pathway, Mallorca, Spain","volume":"28","author":"S Delgado-Serra","year":"2022","journal-title":"Emerg Infect Dis"},{"issue":"12","key":"pntd.0011038.ref004","doi-asserted-by":"crossref","first-page":"2564","DOI":"10.3201\/eid2812.220418","article-title":"Autochthonous Angiostrongylus cantonensis lungworms in urban rats, Valencia, Spain, 2021","volume":"28","author":"MT Gal\u00e1n-Puchades","year":"2022","journal-title":"Emerg Infect Dis"},{"key":"pntd.0011038.ref005","article-title":"Neuroangiostrongyliasis: global spread of an emerging tropical disease","author":"RH Cowie","year":"2022","journal-title":"Am J Trop Med Hyg"},{"issue":"2","key":"pntd.0011038.ref006","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1017\/S003118202000236X","article-title":"Angiostrongylus cantonensis and neuroangiostrongyliasis (rat lungworm disease): 2020","volume":"148","author":"S Jarvi","year":"2021","journal-title":"Parasitology"},{"issue":"5","key":"pntd.0011038.ref007","doi-asserted-by":"crossref","first-page":"3115","DOI":"10.1111\/tbed.14393","article-title":"What do we know about Angiostrongylus cantonensis in Spain? 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