{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T15:06:40Z","timestamp":1793113600004,"version":"4.5.0"},"update-to":[{"DOI":"10.1371\/journal.pntd.0011234","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2023,4,17]],"date-time":"2023-04-17T00:00:00Z","timestamp":1681689600000}}],"reference-count":83,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2023,4,5]],"date-time":"2023-04-05T00:00:00Z","timestamp":1680652800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["2019368"],"award-info":[{"award-number":["2019368"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/V011278\/1"],"award-info":[{"award-number":["BB\/V011278\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R21AI138074"],"award-info":[{"award-number":["R21AI138074"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["DGE-1747503"],"award-info":[{"award-number":["DGE-1747503"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["5T32AI007414-27"],"award-info":[{"award-number":["5T32AI007414-27"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/T001240\/1"],"award-info":[{"award-number":["BB\/T001240\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/W018446\/1"],"award-info":[{"award-number":["BB\/W018446\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014013","name":"UK Research and Innovation","doi-asserted-by":"publisher","award":["20197 and 85336"],"award-info":[{"award-number":["20197 and 85336"]}],"id":[{"id":"10.13039\/100014013","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000266","name":"Engineering and Physical Sciences Research Council","doi-asserted-by":"publisher","award":["V043811\/1"],"award-info":[{"award-number":["V043811\/1"]}],"id":[{"id":"10.13039\/501100000266","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000288","name":"Royal Society","doi-asserted-by":"crossref","award":["RSWF\\R1\\180013"],"award-info":[{"award-number":["RSWF\\R1\\180013"]}],"id":[{"id":"10.13039\/501100000288","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000272","name":"National Institute for Health and Care Research","doi-asserted-by":"publisher","award":["NIHR2000907"],"award-info":[{"award-number":["NIHR2000907"]}],"id":[{"id":"10.13039\/501100000272","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["2018-67012-29991"],"award-info":[{"award-number":["2018-67012-29991"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>Mosquitoes develop in a wide range of aquatic habitats containing highly diverse and variable bacterial communities that shape both larval and adult traits, including the capacity of adult females of some mosquito species to transmit disease-causing organisms to humans. However, while most mosquito studies control for host genotype and environmental conditions, the impact of microbiota variation on phenotypic outcomes of mosquitoes is often unaccounted for. The inability to conduct reproducible intra- and inter-laboratory studies of mosquito-microbiota interactions has also greatly limited our ability to identify microbial targets for mosquito-borne disease control. Here, we developed an approach to isolate and cryopreserve bacterial communities derived from lab and field-based larval rearing environments of the yellow fever mosquito <jats:italic>Aedes aegypti<\/jats:italic>\u2013a primary vector of dengue, Zika, and chikungunya viruses. We then validated the use of our approach to generate experimental microcosms colonized by standardized lab- and field-derived bacterial communities. Our results overall reveal minimal effects of cryopreservation on the recovery of both lab- and field-derived bacteria when directly compared with isolation from non-cryopreserved fresh material. Our results also reveal improved reproducibility of bacterial communities in replicate microcosms generated using cryopreserved stocks over fresh material. Communities in replicate microcosms further captured the majority of total bacterial diversity present in both lab- and field-based larval environments, although the relative richness of recovered taxa as compared to non-recovered taxa was substantially lower in microcosms containing field-derived bacteria. Altogether, these results provide a critical next step toward the standardization of mosquito studies to include larval rearing environments colonized by defined microbial communities. They also lay the foundation for long-term studies of mosquito-microbe interactions and the identification and manipulation of taxa with potential to reduce mosquito vectorial capacity.<\/jats:p>","DOI":"10.1371\/journal.pntd.0011234","type":"journal-article","created":{"date-parts":[[2023,4,5]],"date-time":"2023-04-05T18:14:24Z","timestamp":1680718464000},"page":"e0011234","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":14,"title":["A cryopreservation method to recover laboratory- and field-derived bacterial communities from mosquito larval habitats"],"prefix":"10.1371","volume":"17","author":[{"given":"Serena Y.","family":"Zhao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grant L.","family":"Hughes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2701-5195","authenticated-orcid":true,"given":"Kerri L.","family":"Coon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,4,5]]},"reference":[{"issue":"1","key":"pntd.0011234.ref001","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1186\/s40168-021-01073-2","article-title":"The microbiome and mosquito vectorial capacity: rich potential for discovery and translation.","volume":"9","author":"C Cansado-Utrilla","year":"2021","journal-title":"Microbiome."},{"issue":"9","key":"pntd.0011234.ref002","doi-asserted-by":"crossref","first-page":"e24767","DOI":"10.1371\/journal.pone.0024767","article-title":"Dynamic gut microbiome across life history of the malaria mosquito Anopheles gambiae in Kenya.","volume":"6","author":"Y Wang","year":"2011","journal-title":"PloS One"},{"issue":"11","key":"pntd.0011234.ref003","doi-asserted-by":"crossref","first-page":"2727","DOI":"10.1111\/mec.12771","article-title":"Mosquitoes rely on their gut microbiota for development","volume":"23","author":"KL Coon","year":"2014","journal-title":"Mol Ecol"},{"issue":"16","key":"pntd.0011234.ref004","doi-asserted-by":"crossref","first-page":"3776","DOI":"10.1111\/mec.13730","article-title":"Microbiome evolution along divergent branches of the vertebrate tree of life: what is known and unknown","volume":"25","author":"TJ Colston","year":"2016","journal-title":"Mol Ecol"},{"key":"pntd.0011234.ref005","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1146\/annurev-genet-110711-155532","article-title":"The Relationship Between the Human Genome and Microbiome Comes into View","volume":"51","author":"JK Goodrich","year":"2017","journal-title":"Annu Rev Genet"},{"key":"pntd.0011234.ref006","doi-asserted-by":"crossref","first-page":"14319","DOI":"10.1038\/ncomms14319","article-title":"Unraveling the processes shaping mammalian gut microbiomes over evolutionary time","volume":"8","author":"M Groussin","year":"2017","journal-title":"Nat Commun"},{"issue":"12","key":"pntd.0011234.ref007","doi-asserted-by":"crossref","first-page":"2644","DOI":"10.1038\/ismej.2017.133","article-title":"Network analysis of gut microbiota literature: an overview of the research landscape in non-human animal studies.","volume":"11","author":"EL Pascoe","year":"2017","journal-title":"ISME J"},{"issue":"5","key":"pntd.0011234.ref008","first-page":"762","article-title":"Serratia odorifera mediated enhancement in susceptibility of Aedes aegypti for chikungunya virus","volume":"139","author":"AD Apte-Deshpande","year":"2014","journal-title":"Indian J Med Res"},{"issue":"2","key":"pntd.0011234.ref009","doi-asserted-by":"crossref","first-page":"E176","DOI":"10.1073\/pnas.1412984112","article-title":"Antiviral immunity of Anopheles gambiae is highly compartmentalized, with distinct roles for RNA interference and gut microbiota","volume":"112","author":"G Carissimo","year":"2015","journal-title":"Proc Natl Acad Sci U S A"},{"key":"pntd.0011234.ref010","first-page":"22","article-title":"An epidemiologically successful Escherichia coli sequence type modulates Plasmodium falciparum infection in the mosquito midgut","volume":"43","author":"MT Tchioffo","year":"2016","journal-title":"Infect Genet Evol J Mol Epidemiol Evol Genet Infect Dis"},{"issue":"8","key":"pntd.0011234.ref011","doi-asserted-by":"crossref","first-page":"e1700585","DOI":"10.1126\/sciadv.1700585","article-title":"Carryover effects of larval exposure to different environmental bacteria drive adult trait variation in a mosquito vector.","volume":"3","author":"LB Dickson","year":"2017","journal-title":"Sci Adv"},{"issue":"1","key":"pntd.0011234.ref012","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.chom.2018.11.004","article-title":"A Gut Commensal Bacterium Promotes Mosquito Permissiveness to Arboviruses.","volume":"25","author":"P Wu","year":"2019","journal-title":"Cell Host Microbe"},{"issue":"1","key":"pntd.0011234.ref013","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1186\/s40168-022-01256-5","article-title":"Interspecies microbiome transplantation recapitulates microbial acquisition in mosquitoes","volume":"10","author":"KL Coon","year":"2022","journal-title":"Microbiome"},{"key":"pntd.0011234.ref014","article-title":"Aedes aegypti gut transcriptomes respond differently to microbiome transplants from field-caught or laboratory-reared mosquitoes","author":"S Hegde","year":"2023","journal-title":"Microbiology"},{"issue":"22","key":"pntd.0011234.ref015","doi-asserted-by":"crossref","first-page":"5806","DOI":"10.1111\/mec.13877","article-title":"Mosquitoes host communities of bacteria that are essential for development but vary greatly between local habitats","volume":"25","author":"KL Coon","year":"2016","journal-title":"Mol Ecol"},{"issue":"1","key":"pntd.0011234.ref016","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1186\/s40168-018-0528-y","article-title":"Factors shaping the gut bacterial community assembly in two main Colombian malaria vectors","volume":"6","author":"P Bascu\u00f1\u00e1n","year":"2018","journal-title":"Microbiome"},{"key":"pntd.0011234.ref017","doi-asserted-by":"crossref","first-page":"775078","DOI":"10.3389\/fmicb.2021.775078","article-title":"Microbiota Variation Across Life Stages of European Field-Caught Anopheles atroparvus and During Laboratory Colonization: New Insights for Malaria Research.","volume":"12","author":"L Birnberg","year":"2021","journal-title":"Front Microbiol."},{"issue":"1","key":"pntd.0011234.ref018","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1007\/s00248-020-01544-3","article-title":"Natural Variation in Physicochemical Profiles and Bacterial Communities Associated with Aedes aegypti Breeding Sites and Larvae on Guadeloupe and French Guiana.","volume":"81","author":"L Hery","year":"2021","journal-title":"Microb Ecol."},{"issue":"1","key":"pntd.0011234.ref019","doi-asserted-by":"crossref","first-page":"fiac016","DOI":"10.1093\/femsec\/fiac016","article-title":"Larval habitat determines the bacterial and fungal microbiota of the mosquito vector Aedes aegypti","volume":"98","author":"K Zouache","year":"2022","journal-title":"FEMS Microbiol Ecol"},{"issue":"3","key":"pntd.0011234.ref020","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/S0011-2240(03)00046-4","article-title":"Protectants used in the cryopreservation of microorganisms","volume":"46","author":"Z. Hub\u00e1lek","year":"2003","journal-title":"Cryobiology"},{"issue":"11","key":"pntd.0011234.ref021","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1038\/nrmicro2246","article-title":"Giving microbial diversity a home","volume":"7","author":"D Emerson","year":"2009","journal-title":"Nat Rev Microbiol"},{"issue":"3","key":"pntd.0011234.ref022","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1007\/s00253-011-3797-y","article-title":"Safeguarding bacterial resources promotes biotechnological innovation","volume":"94","author":"K Heylen","year":"2012","journal-title":"Appl Microbiol Biotechnol"},{"issue":"1","key":"pntd.0011234.ref023","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/1574-6968.12034","article-title":"Practice and prospects of microbial preservation","volume":"339","author":"O Prakash","year":"2013","journal-title":"FEMS Microbiol Lett"},{"issue":"6","key":"pntd.0011234.ref024","doi-asserted-by":"crossref","first-page":"e99517","DOI":"10.1371\/journal.pone.0099517","article-title":"Optimized cryopreservation of mixed microbial communities for conserved functionality and diversity.","volume":"9","author":"FM Kerckhof","year":"2014","journal-title":"PloS One"},{"issue":"4","key":"pntd.0011234.ref025","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1111\/1751-7915.13265","article-title":"Effect of cryopreservation and lyophilization on viability and growth of strict anaerobic human gut microbes","volume":"11","author":"L Bircher","year":"2018","journal-title":"Microb Biotechnol"},{"key":"pntd.0011234.ref026","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/j.actatropica.2019.01.005","article-title":"Impact of sugar composition on meal distribution, longevity, and insecticide toxicity in Aedes aegypti","volume":"191","author":"PM Airs","year":"2019","journal-title":"Acta Trop"},{"issue":"2","key":"pntd.0011234.ref027","first-page":"e02141","article-title":"Diet Influences Early Microbiota Development in Dairy Calves without Long-Term Impacts on Milk Production.","volume":"85","author":"KA Dill-McFarland","year":"2019","journal-title":"Appl Environ Microbiol"},{"issue":"17","key":"pntd.0011234.ref028","doi-asserted-by":"crossref","first-page":"5112","DOI":"10.1128\/AEM.01043-13","article-title":"Development of a Dual-Index Sequencing Strategy and Curation Pipeline for Analyzing Amplicon Sequence Data on the MiSeq Illumina Sequencing Platform","volume":"79","author":"JJ Kozich","year":"2013","journal-title":"Appl Environ Microbiol"},{"issue":"8","key":"pntd.0011234.ref029","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1038\/s41587-019-0209-9","article-title":"Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2","volume":"37","author":"E Bolyen","year":"2019","journal-title":"Nat Biotechnol"},{"issue":"7","key":"pntd.0011234.ref030","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1038\/nmeth.3869","article-title":"DADA2: High-resolution sample inference from Illumina amplicon data.","volume":"13","author":"BJ Callahan","year":"2016","journal-title":"Nat Methods.Jul"},{"issue":"D1","key":"pntd.0011234.ref031","doi-asserted-by":"crossref","first-page":"D590","DOI":"10.1093\/nar\/gks1219","article-title":"The SILVA ribosomal RNA gene database project: improved data processing and web-based tools","volume":"41","author":"C Quast","year":"2012","journal-title":"Nucleic Acids Res"},{"issue":"3","key":"pntd.0011234.ref032","doi-asserted-by":"crossref","first-page":"e9490","DOI":"10.1371\/journal.pone.0009490","article-title":"FastTree 2 \u2013Approximately Maximum-Likelihood Trees for Large Alignments.","volume":"5","author":"MN Price","year":"2010","journal-title":"PLoS ONE"},{"issue":"4","key":"pntd.0011234.ref033","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1093\/molbev\/mst010","article-title":"MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability","volume":"30","author":"K Katoh","year":"2013","journal-title":"Mol Biol Evol"},{"issue":"4","key":"pntd.0011234.ref034","doi-asserted-by":"crossref","first-page":"e61217","DOI":"10.1371\/journal.pone.0061217","article-title":"phyloseq: An R Package for Reproducible Interactive Analysis and Graphics of Microbiome Census Data.","volume":"8","author":"PJ McMurdie","year":"2013","journal-title":"PLoS ONE"},{"issue":"1","key":"pntd.0011234.ref035","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1186\/s40168-018-0605-2","article-title":"Simple statistical identification and removal of contaminant sequences in marker-gene and metagenomics data.","volume":"6","author":"NM Davis","year":"2018","journal-title":"Microbiome."},{"key":"pntd.0011234.ref036","author":"J. Oksanen","year":"2022","journal-title":"vegan: Community Ecology Package"},{"issue":"7","key":"pntd.0011234.ref037","doi-asserted-by":"crossref","first-page":"e67019","DOI":"10.1371\/journal.pone.0067019","article-title":"ANOVA-Like Differential Expression (ALDEx) Analysis for Mixed Population RNA-Seq.","volume":"8","author":"AD Fernandes","year":"2013","journal-title":"PLoS ONE"},{"issue":"3","key":"pntd.0011234.ref038","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1080\/10618600.2015.1131161","article-title":"Displaying Variation in Large Datasets: Plotting a Visual Summary of Effect Sizes","volume":"25","author":"GB Gloor","year":"2016","journal-title":"J Comput Graph Stat"},{"issue":"1","key":"pntd.0011234.ref039","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1038\/s41467-022-28034-z","article-title":"Microbiome differential abundance methods produce different results across 38 datasets.","volume":"13","author":"JT Nearing","year":"2022","journal-title":"Nat Commun."},{"key":"pntd.0011234.ref040","article-title":"The control of the false discovery rate in multiple testing under dependency.","author":"Y Benjamini","year":"2001","journal-title":"Ann Stat"},{"key":"pntd.0011234.ref041","article-title":"A cryopreservation method to recover laboratory- and field-derived bacterial communities from mosquito larval habitats","author":"S Zhao","year":"2023","journal-title":"Dryad Digital Repository"},{"issue":"3","key":"pntd.0011234.ref042","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1111\/j.1574-6941.2010.01012.x","article-title":"Bacterial diversity of field-caught mosquitoes, Aedes albopictus and Aedes aegypti, from different geographic regions of Madagascar","volume":"75","author":"K Zouache","year":"2011","journal-title":"FEMS Microbiol Ecol"},{"issue":"1","key":"pntd.0011234.ref043","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1186\/s13071-018-3036-9","article-title":"Mosquito microbiota cluster by host sampling location.","volume":"11","author":"EJ Muturi","year":"2018","journal-title":"Parasit Vectors."},{"key":"pntd.0011234.ref044","doi-asserted-by":"crossref","first-page":"596750","DOI":"10.3389\/fcimb.2020.596750","article-title":"Comparative Analysis of the Gut Microbiota of Adult Mosquitoes From Eight Locations in Hainan, China.","volume":"10","author":"X Kang","year":"2020","journal-title":"Front Cell Infect Microbiol."},{"issue":"8","key":"pntd.0011234.ref045","doi-asserted-by":"crossref","first-page":"e0008615","DOI":"10.1371\/journal.pntd.0008615","article-title":"The Aedes albopictus (Diptera: Culicidae) microbiome varies spatially and with Ascogregarine infection.","volume":"14","author":"P Seabourn","year":"2020","journal-title":"PLoS Negl Trop Dis"},{"key":"pntd.0011234.ref046","article-title":"Mosquito Microbiome Diversity Varies Along a Landscape-Scale Moisture Gradient.","author":"MCI Medeiros","year":"2021","journal-title":"Microb Ecol"},{"key":"pntd.0011234.ref047","article-title":"Seasonal variation of microbiota composition in Anopheles gambiae and Anopheles coluzzii in two different eco-geographical localities in Cameroon","author":"MM Sandeu","year":"2022","journal-title":"Med Vet Entomol"},{"issue":"5","key":"pntd.0011234.ref048","doi-asserted-by":"crossref","first-page":"e1002742","DOI":"10.1371\/journal.ppat.1002742","article-title":"Midgut microbiota of the malaria mosquito vector Anopheles gambiae and interactions with Plasmodium falciparum infection.","volume":"8","author":"A Boissi\u00e8re","year":"2012","journal-title":"PLoS Pathog"},{"key":"pntd.0011234.ref049","doi-asserted-by":"crossref","first-page":"22806","DOI":"10.1038\/srep22806","article-title":"Bacterial associations reveal spatial population dynamics in Anopheles gambiae mosquitoes.","volume":"6","author":"M Buck","year":"2016","journal-title":"Sci Rep."},{"issue":"1","key":"pntd.0011234.ref050","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1186\/s13071-018-2780-1","article-title":"Diverse laboratory colonies of Aedes aegypti harbor the same adult midgut bacterial microbiome.","volume":"11","author":"LB Dickson","year":"2018","journal-title":"Parasit Vectors."},{"issue":"7","key":"pntd.0011234.ref051","doi-asserted-by":"crossref","first-page":"e0006638","DOI":"10.1371\/journal.pntd.0006638","article-title":"Both living bacteria and eukaryotes in the mosquito gut promote growth of larvae","volume":"12","author":"L Valzania","year":"2018","journal-title":"PLoS Negl Trop Dis"},{"issue":"1","key":"pntd.0011234.ref052","doi-asserted-by":"crossref","first-page":"4464","DOI":"10.1038\/s41467-018-07014-2","article-title":"Generation of axenic Aedes aegypti demonstrate live bacteria are not required for mosquito development","volume":"9","author":"MA Correa","year":"2018","journal-title":"Nat Commun"},{"issue":"1","key":"pntd.0011234.ref053","doi-asserted-by":"crossref","first-page":"942","DOI":"10.1038\/s41467-021-21195-3","article-title":"Production of germ-free mosquitoes via transient colonisation allows stage-specific investigation of host\u2013microbiota interactions.","volume":"12","author":"O Romoli","year":"2021","journal-title":"Nat Commun."},{"issue":"6","key":"pntd.0011234.ref054","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.2307\/3281096","article-title":"Anopheles stephensi and Toxorhynchites amboinensis: aseptic rearing of mosquito larvae on cultured cells","volume":"68","author":"UG Munderloh","year":"1982","journal-title":"J Parasitol"},{"issue":"5","key":"pntd.0011234.ref055","doi-asserted-by":"crossref","first-page":"1529","DOI":"10.2307\/1940953","article-title":"Nutrient Dynamics, Bacterial Populations, and Mosquito Productivity in Tree Hole Ecosystems and Microcosms","volume":"72","author":"ED Walker","year":"1991","journal-title":"Ecology"},{"issue":"6","key":"pntd.0011234.ref056","doi-asserted-by":"crossref","first-page":"2661","DOI":"10.1128\/AEM.65.6.2661-2673.1999","article-title":"Effects of Larval Mosquitoes (Aedes triseriatus) and Stemflow on Microbial Community Dynamics in Container Habitats.","volume":"65","author":"MG Kaufman","year":"1999","journal-title":"Appl Environ Microbiol"},{"issue":"6","key":"pntd.0011234.ref057","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1603\/0022-2585-40.6.841","article-title":"Role of Bacteria in Mediating the Oviposition Responses of Aedes albopictus (Diptera: Culicidae).","volume":"40","author":"JD Trexler","year":"2003","journal-title":"J Med Entomol"},{"issue":"6","key":"pntd.0011234.ref058","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1093\/jmedent\/45.6.1039","article-title":"Oviposition Responses of Anopheles gambiae s.s. (Diptera: Culicidae) and Identification of Volatiles from Bacteria-Containing Solutions","volume":"45","author":"JM Lindh","year":"2008","journal-title":"J Med Entomol"},{"issue":"9","key":"pntd.0011234.ref059","doi-asserted-by":"crossref","first-page":"e24409","DOI":"10.1371\/journal.pone.0024409","article-title":"Bacteria Stimulate Hatching of Yellow Fever Mosquito Eggs.","volume":"6","author":"L Ponnusamy","year":"2011","journal-title":"PLoS ONE"},{"key":"pntd.0011234.ref060","doi-asserted-by":"crossref","first-page":"2160","DOI":"10.3389\/fmicb.2018.02160","article-title":"Microbiome Interaction Networks and Community Structure From Laboratory-Reared and Field-Collected Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus Mosquito Vectors.","volume":"9","author":"S Hegde","year":"2018","journal-title":"Front Microbiol."},{"issue":"1","key":"pntd.0011234.ref061","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1186\/s12866-015-0475-8","article-title":"Developmental succession of the microbiome of Culex mosquitoes","volume":"15","author":"D Duguma","year":"2015","journal-title":"BMC Microbiol"},{"issue":"16","key":"pntd.0011234.ref062","doi-asserted-by":"crossref","first-page":"4075","DOI":"10.1111\/mec.13741","article-title":"Midgut fungal and bacterial microbiota of Aedes triseriatus and Aedes japonicus shift in response to La Crosse virus infection","volume":"25","author":"EJ Muturi","year":"2016","journal-title":"Mol Ecol"},{"key":"pntd.0011234.ref063","article-title":"Comparative analysis of gut microbiota of mosquito communities in central Illinois","author":"EJ Muturi","year":"2017","journal-title":"PLoS Negl Trop Dis"},{"issue":"2","key":"pntd.0011234.ref064","doi-asserted-by":"crossref","first-page":"1352","DOI":"10.1002\/ece3.3676","article-title":"Mosquito vector-associated microbiota: Metabarcoding bacteria and eukaryotic symbionts across habitat types in Thailand endemic for dengue and other arthropod-borne diseases.","volume":"8","author":"P Thongsripong","year":"2018","journal-title":"Ecol Evol."},{"issue":"20","key":"pntd.0011234.ref065","doi-asserted-by":"crossref","first-page":"5138","DOI":"10.1111\/j.1365-294X.2012.05759.x","article-title":"Deep sequencing reveals extensive variation in the gut microbiota of wild mosquitoes from Kenya","volume":"21","author":"J Osei-Poku","year":"2012","journal-title":"Mol Ecol"},{"issue":"9","key":"pntd.0011234.ref066","doi-asserted-by":"crossref","first-page":"1836","DOI":"10.1016\/j.watres.2006.03.002","article-title":"Long-term storage conditions for carriers with denitrifying biomass of the fluidized, methanol-fed denitrification reactor of the Montreal Biodome, and the impact on denitrifying activity and bacterial population","volume":"40","author":"V Laurin","year":"2006","journal-title":"Water Res"},{"issue":"6","key":"pntd.0011234.ref067","doi-asserted-by":"crossref","first-page":"1376","DOI":"10.1007\/s00253-006-0770-2","article-title":"Reactivation of aerobic and anaerobic ammonium oxidizers in OLAND biomass after long-term storage","volume":"74","author":"SE Vlaeminck","year":"2007","journal-title":"Appl Microbiol Biotechnol"},{"issue":"5","key":"pntd.0011234.ref068","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1038\/ajg.2011.482","article-title":"Standardized frozen preparation for transplantation of fecal microbiota for recurrent Clostridium difficile infection","volume":"107","author":"MJ Hamilton","year":"2012","journal-title":"Am J Gastroenterol"},{"key":"pntd.0011234.ref069","doi-asserted-by":"crossref","first-page":"16350","DOI":"10.1038\/srep16350","article-title":"Sample storage conditions significantly influence faecal microbiome profiles.","volume":"5","author":"JM Choo","year":"2015","journal-title":"Sci Rep."},{"issue":"2","key":"pntd.0011234.ref070","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1089\/bio.2015.0022","article-title":"Preservation of Biospecimens at Ambient Temperature: Special Focus on Nucleic Acids and Opportunities for the Biobanking Community.","volume":"14","author":"R Muller","year":"2016","journal-title":"Biopreservation Biobanking."},{"issue":"3","key":"pntd.0011234.ref071","first-page":"e00021","article-title":"Preservation Methods Differ in Fecal Microbiome Stability, Affecting Suitability for Field Studies.","volume":"1","author":"SJ Song","year":"2016","journal-title":"mSystems"},{"key":"pntd.0011234.ref072","first-page":"67","volume-title":"Manual of clinical microbiology.","author":"L Reimer","year":"2004"},{"issue":"1","key":"pntd.0011234.ref073","doi-asserted-by":"crossref","first-page":"1317","DOI":"10.1038\/s41467-019-09267-x","article-title":"Assessing the viability of transplanted gut microbiota by sequential tagging with D-amino acid-based metabolic probes","volume":"10","author":"W Wang","year":"2019","journal-title":"Nat Commun"},{"issue":"1","key":"pntd.0011234.ref074","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1186\/s40168-021-01060-7","article-title":"Strain engraftment competition and functional augmentation in a multi-donor fecal microbiota transplantation trial for obesity","volume":"9","author":"BC Wilson","year":"2021","journal-title":"Microbiome"},{"key":"pntd.0011234.ref075","doi-asserted-by":"crossref","first-page":"143","DOI":"10.3354\/ame022143","article-title":"Heterogeneity in bacterioplankton abundance from 4.5 millimetre resolution sampling.","volume":"22","author":"J Seymour","year":"2000","journal-title":"Aquat Microb Ecol"},{"key":"pntd.0011234.ref076","doi-asserted-by":"crossref","first-page":"103","DOI":"10.3354\/ame026103","article-title":"Microscale patchiness of bacterioplankton assemblage richness in seawater.","volume":"26","author":"R Long","year":"2001","journal-title":"Aquat Microb Ecol"},{"key":"pntd.0011234.ref077","article-title":"Size Matters: Assessing Optimum Soil Sample Size for Fungal and Bacterial Community Structure Analyses Using High Throughput Sequencing of rRNA Gene Amplicons.","author":"CR Penton","year":"2016","journal-title":"Front Microbiol"},{"key":"pntd.0011234.ref078","volume-title":"Microbial Ecology of the Oceans.","author":"DL Kirchman","year":"2010"},{"issue":"2","key":"pntd.0011234.ref079","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1038\/ismej.2012.115","article-title":"Hydration dynamics promote bacterial coexistence on rough surfaces.","volume":"7","author":"G Wang","year":"2013","journal-title":"ISME J."},{"issue":"11","key":"pntd.0011234.ref080","doi-asserted-by":"crossref","first-page":"3398","DOI":"10.1111\/1462-2920.12388","article-title":"Microscale sulfur cycling in the phototrophic pink berry consortia of the Sippewissett Salt Marsh","volume":"16","author":"EG Wilbanks","year":"2014","journal-title":"Environ Microbiol"},{"key":"pntd.0011234.ref081","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.soilbio.2017.12.018","article-title":"Greatest soil microbial diversity found in micro-habitats","volume":"118","author":"EM Bach","year":"2018","journal-title":"Soil Biol Biochem"},{"issue":"7054","key":"pntd.0011234.ref082","doi-asserted-by":"crossref","first-page":"1157","DOI":"10.1038\/nature03891","article-title":"The contribution of species richness and composition to bacterial services","volume":"436","author":"T Bell","year":"2005","journal-title":"Nature"},{"issue":"4","key":"pntd.0011234.ref083","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1038\/ismej.2016.174","article-title":"Where less may be more: how the rare biosphere pulls ecosystems strings.","volume":"11","author":"A Jousset","year":"2017","journal-title":"ISME J."}],"updated-by":[{"DOI":"10.1371\/journal.pntd.0011234","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2023,4,17]],"date-time":"2023-04-17T00:00:00Z","timestamp":1681689600000}}],"container-title":["PLOS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0011234","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,17]],"date-time":"2023-04-17T17:41:00Z","timestamp":1681753260000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0011234"}},"subtitle":[],"editor":[{"given":"Lyric C.","family":"Bartholomay","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2023,4,5]]},"references-count":83,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2023,4,5]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0011234","relation":{"new_version":[{"id-type":"doi","id":"10.1371\/journal.pntd.0011234","asserted-by":"object"}]},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,5]]}}}