{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T11:55:06Z","timestamp":1767182106815,"version":"build-2238731810"},"update-to":[{"DOI":"10.1371\/journal.pntd.0011321","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2023,6,7]],"date-time":"2023-06-07T00:00:00Z","timestamp":1686096000000}}],"reference-count":55,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2023,5,25]],"date-time":"2023-05-25T00:00:00Z","timestamp":1684972800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010751","name":"Fondo Nacional de Desarrollo Cient\u00edfico, Tecnol\u00f3gico y de Innovaci\u00f3n Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["Agreement N\u00b0 403-2019-FONDECYT"],"award-info":[{"award-number":["Agreement N\u00b0 403-2019-FONDECYT"]}],"id":[{"id":"10.13039\/501100010751","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>\n                    Carrion\u00b4s disease is caused by\n                    <jats:italic>Bartonella bacilliformis<\/jats:italic>\n                    , it is a Gram-negative pleomorphic bacterium.\n                    <jats:italic>B<\/jats:italic>\n                    .\n                    <jats:italic>bacilliformis<\/jats:italic>\n                    is transmitted by\n                    <jats:italic>Lutzomyia verrucarum<\/jats:italic>\n                    in endemic areas of the Peruvian Inter-Andean valleys. Additionally, the pathogenicity of\n                    <jats:italic>B<\/jats:italic>\n                    .\n                    <jats:italic>bacilliformis<\/jats:italic>\n                    involves an initial infection of erythrocytes and the further infection of endothelial cells, which mainly affects children and expectant women from extreme poverty rural areas. Therefore, the implementation of serological diagnostic methods and the development of candidate vaccines for the control of CD could be facilitated by the prediction of linear b-cell epitopes in specific proteins of\n                    <jats:italic>B<\/jats:italic>\n                    .\n                    <jats:italic>bacilliformis<\/jats:italic>\n                    by bioinformatics analysis. In this study, We used an\n                    <jats:italic>in-silico<\/jats:italic>\n                    analysis employing six web servers for the identification of epitopes in proteins of\n                    <jats:italic>B<\/jats:italic>\n                    .\n                    <jats:italic>bacilliformis<\/jats:italic>\n                    . The selection of\n                    <jats:italic>B<\/jats:italic>\n                    .\n                    <jats:italic>bacilliformis-<\/jats:italic>\n                    specific proteins and their analysis to identify epitopes allowed the selection of seven protein candidates that are expected to have high antigenic activity.\n                  <\/jats:p>","DOI":"10.1371\/journal.pntd.0011321","type":"journal-article","created":{"date-parts":[[2023,5,25]],"date-time":"2023-05-25T13:43:58Z","timestamp":1685022238000},"page":"e0011321","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":5,"title":["In-silico identification of linear B-cell epitopes in specific proteins of Bartonella bacilliformis for the serological diagnosis of Carrion\u2019s disease"],"prefix":"10.1371","volume":"17","author":[{"given":"Victor","family":"Jimenez-Vasquez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0438-9602","authenticated-orcid":true,"given":"Karen Daphne","family":"Calvay-Sanchez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanina","family":"Zarate-Sulca","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giovanna","family":"Mendoza-Mujica","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2023,5,25]]},"reference":[{"key":"pntd.0011321.ref001","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pntd.0001819","article-title":"Bartonella bacilliformis: A systematic review of the literature to guide the research agenda for elimination","volume":"6","author":"N Sanchez Clemente","year":"2012","journal-title":"PLOS Neglected Tropical Diseases"},{"key":"pntd.0011321.ref002","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/CMR.00056-17","article-title":"Carrion\u2019s Disease: the sound of silence","volume":"31","author":"C Gomes","year":"2018","journal-title":"Clin Microbiol Rev"},{"key":"pntd.0011321.ref003","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1590\/S0036-46652003000100015","article-title":"Bartonellosis o enfermedad de Carri\u00f3n: nuevos aspectos de una vieja enfermedad","volume":"45","author":"C. 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