{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T02:34:37Z","timestamp":1772505277022,"version":"3.50.1"},"reference-count":54,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2024,5,28]],"date-time":"2024-05-28T00:00:00Z","timestamp":1716854400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["T35DK062719-30"],"award-info":[{"award-number":["T35DK062719-30"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100015691","name":"Division of Microbiology and Infectious Diseases, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01 AI2753,RO1 16945, AI08749-01A1 BIOL-3, U01 AI77887, U01 AI082180, TMP R01- AI071319"],"award-info":[{"award-number":["R01 AI2753,RO1 16945, AI08749-01A1 BIOL-3, U01 AI77887, U01 AI082180, TMP R01- AI071319"]}],"id":[{"id":"10.13039\/100015691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005627","name":"Thrasher Research Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005627","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the Kiphart Global-Local Health Seed Fund Award"},{"DOI":"10.13039\/100007234","name":"University of Chicago","doi-asserted-by":"publisher","award":["Provost Award for Latin America and the Carribean"],"award-info":[{"award-number":["Provost Award for Latin America and the Carribean"]}],"id":[{"id":"10.13039\/100007234","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Taking out Toxo"},{"name":"Network for Good"},{"name":"Toxoplasmosis Research Institute"},{"name":"Cornwell Mann family foundation"},{"name":"Rodriguez family"},{"name":"The Samuel family and Running for Fin"},{"name":"the Morel, Rooney, Mussalami, Kapnick, Taub, Engel, Harris, Drago, Longfellow\/Van Dusen families"}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Background<\/jats:title>\n<jats:p>Congenital toxoplasmosis is a treatable, preventable disease, but untreated causes death, prematurity, loss of sight, cognition and motor function, and substantial costs worldwide.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Objectives<\/jats:title>\n<jats:p>We asked whether high performance of an Immunochromatographic-test (ICT) could enable accurate, rapid diagnosis\/treatment, establishing new, improved care-paradigms at point-of-care and clinical laboratory.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Methods<\/jats:title>\n<jats:p>Data were obtained in 12 studies\/analyses addressing: 1-feasibility\/efficacy; 2-false-positives; 3-acceptability; 4-pink\/black-line\/all studies; 5-time\/cost; 6-Quick-Information\/Limit-of-detection; 7, 8-acute;-chronic; 9-epidemiology; 10-ADBio; 11,12-Commentary\/Cases\/Chronology.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec004\">\n<jats:title>Findings<\/jats:title>\n<jats:p>ICT was compared with gold-standard or predicate-tests. Overall, ICT performance for 1093 blood\/4967 sera was 99.2%\/97.5% sensitive and 99.0%\/99.7% specific. However, in clinical trial, FDA-cleared-predicate tests initially caused practical, costly problems due to false-positive-IgM results. For 58 persons, 3\/43 seronegative and 2\/15 chronically infected persons had false positive IgM predicate tests. This caused substantial anxiety, concerns, and required costly, delayed confirmation in reference centers. Absence of false positive ICT results contributes to solutions: Lyon and Paris France and USA Reference laboratories frequently receive sera with erroneously positive local laboratory IgM results impeding patient care. Therefore, thirty-two such sera referred to Lyon\u2019s Reference laboratory were ICT-tested. We collated these with other earlier\/ongoing results: 132 of 137 USA or French persons had false-positive local laboratory IgM results identified correctly as negative by ICT. Five false positive ICT results in Tunisia and Marseille, France, emphasize need to confirm positive ICT results with Sabin-Feldman-Dye-test or western blot. Separate studies demonstrated high performance in detecting acute infections, meeting FDA, CLIA, WHO REASSURED, CEMark criteria and patient and physician satisfaction with monthly-gestational-ICT-screening.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec005\">\n<jats:title>Conclusions\/significance<\/jats:title>\n<jats:p>This novel paradigm using ICT identifies likely false positives or raises suspicion that a result is truly positive, rapidly needing prompt follow up and treatment. Thus, ICT enables well-accepted gestational screening programs that facilitate rapid treatment saving lives, sight, cognition and motor function. This reduces anxiety, delays, work, and cost at point-of-care and clinical laboratories.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec006\">\n<jats:title>Trial registration<\/jats:title>\n<jats:p><jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/clinicaltrials.gov\/ct2\/show\/NCT04474132\" xlink:type=\"simple\">NCT04474132<\/jats:ext-link>, <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/clinicaltrials.gov\/study\/NCT04474132\" xlink:type=\"simple\">https:\/\/clinicaltrials.gov\/study\/NCT04474132<\/jats:ext-link><\/jats:p>\n<jats:p><jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/clinicaltrials.gov\/ct2\/show\/ClinicalTrials.gov\" xlink:type=\"simple\">ClinicalTrials.gov<\/jats:ext-link><\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pntd.0011335","type":"journal-article","created":{"date-parts":[[2024,5,28]],"date-time":"2024-05-28T18:38:26Z","timestamp":1716921506000},"page":"e0011335","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":8,"title":["Novel paradigm enables accurate monthly gestational screening to prevent congenital toxoplasmosis and more"],"prefix":"10.1371","volume":"18","author":[{"given":"Ying","family":"Zhou","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen","family":"Leahy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Grose","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph","family":"Lykins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maryam","family":"Siddiqui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole","family":"Leong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Perpetua","family":"Goodall","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shawn","family":"Withers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin","family":"Ashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen","family":"Schrantz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vera","family":"Tesic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Precy","family":"Abeleda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathleen","family":"Beavis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fatima","family":"Clouser","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahmoud","family":"Ismail","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monica","family":"Christmas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raphael","family":"Piarroux","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denis","family":"Limonne","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emmanuelle","family":"Chapey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sylvie","family":"Abraham","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabelle","family":"Baird","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juliette","family":"Thibodeau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenneth M.","family":"Boyer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth","family":"Torres","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shannon","family":"Conrey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kanix","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary Allen","family":"Staat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nancy","family":"Back","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Coralie","family":"L\u2019Ollivier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caroline","family":"Mahinc","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierre","family":"Flori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge","family":"Gomez-Marin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francois","family":"Peyron","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandrine","family":"Houz\u00e9","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martine","family":"Wallon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1130-9631","authenticated-orcid":true,"given":"Rima","family":"McLeod","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2024,5,28]]},"reference":[{"key":"pntd.0011335.ref001","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1590\/S0074-02762009000200029","article-title":"Why prevent, diagnose and treat congenital toxoplasmosis?","volume":"104","author":"R McLeod","year":"2009","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"pntd.0011335.ref002","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1007\/s40124-014-0055-7","article-title":"Management of Congenital Toxoplasmosis","volume":"2","author":"R McLeod","year":"2014","journal-title":"Curr Pediatr Rep"},{"issue":"4","key":"pntd.0011335.ref003","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1016\/j.ajog.2018.05.031","article-title":"Prenatal therapy with pyrimethamine + sulfadiazine vs spiramycin to reduce placental transmission of toxoplasmosis: a multicenter, randomized trial","volume":"219","author":"L Mandelbrot","year":"2018","journal-title":"Am J Obstet Gynecol"},{"key":"pntd.0011335.ref004","doi-asserted-by":"crossref","first-page":"1385","DOI":"10.1016\/j.ijpara.2009.04.003","article-title":"Toxoplasmosis snapshots: global status of Toxoplasma gondii seroprevalence and implications for pregnancy and congenital toxoplasmosis","volume":"39","author":"G Pappas","year":"2009","journal-title":"Int J Parasitol"},{"key":"pntd.0011335.ref005","doi-asserted-by":"crossref","first-page":"501","DOI":"10.2471\/BLT.12.111732","article-title":"The global burden of congenital toxoplasmosis: a systematic review","volume":"91","author":"PR Torgerson","year":"2013","journal-title":"Bull World Health Organ"},{"key":"pntd.0011335.ref006","first-page":"1723","volume-title":"Nelson Textbook of Pediatrics","author":"New edition in press","year":"2024","edition":"2"},{"key":"pntd.0011335.ref007","first-page":"117","volume-title":"Toxoplasma gondii","author":"Human Toxoplasma Infection","year":"2020","edition":"3"},{"key":"pntd.0011335.ref008","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1038\/s41426-018-0164-4","article-title":"Global initiative for congenital toxoplasmosis: an observational and international comparative clinical analysis","volume":"7","author":"K El Bissati","year":"2018","journal-title":"Emerg Microbes Infect"},{"key":"pntd.0011335.ref009","doi-asserted-by":"crossref","first-page":"1223","DOI":"10.1093\/cid\/cit032","article-title":"Congenital Toxoplasma Infection: Monthly Prenatal Screening Decreases Transmission Rate and Improves Clinical Outcome at Age 3 Years","volume":"56","author":"M Wallon","year":"2013","journal-title":"Clin Infect Dis"},{"issue":"2","key":"pntd.0011335.ref010","doi-asserted-by":"crossref","first-page":"180","DOI":"10.4269\/ajtmh.1997.57.180","article-title":"A maternal screening program for congenital toxoplasmosis in Quindio, Colombia and application of mathematical models to estimate incidences using age-stratified data","volume":"57","author":"JE Gomez-Marin","year":"1997","journal-title":"Am J Trop Med Hyg"},{"issue":"4","key":"pntd.0011335.ref011","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1093\/infdis\/jiw211","article-title":"Reply to G\u00f3mez Mar\u00edn","volume":"214","author":"AC Carneiro","year":"2016","journal-title":"J Infect Dis"},{"issue":"11","key":"pntd.0011335.ref012","doi-asserted-by":"crossref","first-page":"1595","DOI":"10.1093\/cid\/cis258","article-title":"Prematurity and severity are associated with Toxoplasma gondii alleles (NCCCTS, 1981\u20132009)","volume":"54","author":"R McLeod","year":"2012","journal-title":"Clin Infect Dis"},{"key":"pntd.0011335.ref013","doi-asserted-by":"crossref","first-page":"e0005222","DOI":"10.1371\/journal.pntd.0005222","article-title":"Congenital Toxoplasmosis in France and the United States: One Parasite, Two Diverging Approaches","volume":"11","author":"F Peyron","year":"2017","journal-title":"PLoS Negl Trop Dis"},{"key":"pntd.0011335.ref014","doi-asserted-by":"crossref","first-page":"e1333","DOI":"10.1371\/journal.pntd.0001333","article-title":"Maternal Serologic Screening to Prevent Congenital Toxoplasmosis: A Decision-Analytic Economic Model","volume":"5","author":"E Stillwaggon","year":"2011","journal-title":"PLoS Negl Trop Dis"},{"key":"pntd.0011335.ref015","doi-asserted-by":"crossref","first-page":"e0005648","DOI":"10.1371\/journal.pntd.0005648","article-title":"Congenital toxoplasmosis in Austria: Prenatal screening for prevention is cost-saving","volume":"11","author":"A-R Prusa","year":"2017","journal-title":"PLoS Negl Trop Dis"},{"issue":"9","key":"pntd.0011335.ref016","doi-asserted-by":"crossref","first-page":"e0221709","DOI":"10.1371\/journal.pone.0221709","article-title":"The cost-effectiveness of neonatal versus prenatal screening for congenital toxoplasmosis","volume":"14","author":"C Binquet","year":"2019","journal-title":"PLoS One"},{"issue":"11","key":"pntd.0011335.ref017","doi-asserted-by":"crossref","first-page":"e0273781","DOI":"10.1371\/journal.pone.0273781","article-title":"Prevention of congenital toxoplasmosis in France using prenatal screening: A decision-analytic economic model","volume":"17","author":"L Sawers","year":"2022","journal-title":"PLoS One"},{"issue":"2","key":"pntd.0011335.ref018","doi-asserted-by":"crossref","first-page":"e20163860","DOI":"10.1542\/peds.2016-3860","article-title":"Committee on Infectious Diseases. Diagnosis, treatment, and prevention of congenital toxoplasmosis in the United States","volume":"139","author":"YA Maldonado","year":"2017","journal-title":"Pediatrics"},{"issue":"9","key":"pntd.0011335.ref019","doi-asserted-by":"crossref","first-page":"639","DOI":"10.2471\/BLT.16.187468","article-title":"A guide to aid the selection of diagnostic tests","volume":"95","author":"CS Kosack","year":"2017","journal-title":"Bull World Health Organ"},{"issue":"1","key":"pntd.0011335.ref020","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1128\/jcm.35.1.174-178.1997","article-title":"False-positive results in immunoglobulin M (IgM) toxoplasma antibody tests and importance of confirmatory testing: the Platelia Toxo IgM test","volume":"35","author":"O Liesenfeld","year":"1997","journal-title":"J Clin Microbiol"},{"issue":"3","key":"pntd.0011335.ref021","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1080\/10647440500148024","article-title":"Commercial laboratory IgM testing for Toxoplasma gondii in pregnancy: a 20-year experience","volume":"13","author":"DJ Garry","year":"2005","journal-title":"Infect Dis Obstet Gynecol"},{"issue":"11","key":"pntd.0011335.ref022","doi-asserted-by":"crossref","first-page":"3601","DOI":"10.1128\/JCM.01663-15","article-title":"Significance of a Positive Toxoplasma Immunoglobulin M Test Result in the United States","volume":"53","author":"R Dhakal","year":"2015","journal-title":"J Clin Microbiol"},{"key":"pntd.0011335.ref023","doi-asserted-by":"crossref","first-page":"e0006536","DOI":"10.1371\/journal.pntd.0006536","article-title":"Rapid, inexpensive, fingerstick, whole-blood, sensitive, specific, point-of-care test for anti-Toxoplasma antibodies","volume":"12","author":"JD Lykins","year":"2018","journal-title":"PLoS Negl Trop Dis"},{"key":"pntd.0011335.ref024","doi-asserted-by":"crossref","first-page":"e0005670","DOI":"10.1371\/journal.pntd.0005670","article-title":"Point-of-care testing for Toxoplasma gondii IgG\/IgM using Toxoplasma ICT IgG-IgM test with sera from the United States and implications for developing countries","volume":"11","author":"IJ Begeman","year":"2017","journal-title":"PLoS Negl Trop Dis"},{"key":"pntd.0011335.ref025","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1016\/j.cca.2016.10.023","article-title":"Evaluation of the LDBIO point of care test for the combined detection of toxoplasmic IgG and IgM","volume":"464","author":"E Chapey","year":"2017","journal-title":"Clin Chim Acta"},{"key":"pntd.0011335.ref026","doi-asserted-by":"crossref","first-page":"3395","DOI":"10.1128\/JCM.01106-17","article-title":"Evaluation of a New Immunochromatography Technology Test (LDBio Diagnostics) To Detect Toxoplasma IgG and IgM: Comparison with the Routine Architect Technique","volume":"55","author":"C Mahinc","year":"2017","journal-title":"J Clin Microbiol"},{"key":"pntd.0011335.ref027","article-title":"Evaluation of Three Point-of-Care Tests for Detection of Toxoplasma Immunoglobulin IgG and IgM in the United States: Proof of Concept and Challenges","volume":"5","author":"CA Gomez","year":"2018","journal-title":"Open For Inf Dis"},{"issue":"4","key":"pntd.0011335.ref028","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1093\/cid\/ciw356","article-title":"Understanding Toxoplasmosis in the United States Through \u201cLarge Data\u201d Analyses","volume":"63","author":"J Lykins","year":"2016","journal-title":"Clin Inf Dis"},{"issue":"1","key":"pntd.0011335.ref029","doi-asserted-by":"crossref","first-page":"1675","DOI":"10.1080\/22221751.2021.1948359","article-title":"Performance of a novel point-of- care blood test for Toxoplasma infection in women from diverse regions of Morocco","volume":"10","author":"B El Mansouri","year":"2021","journal-title":"Microbes Infect"},{"issue":"11","key":"pntd.0011335.ref030","doi-asserted-by":"crossref","first-page":"1327","DOI":"10.1007\/s10096-023-04669-8","volume":"42","author":"S Abraham","year":"2023","journal-title":"European Journal of Clinical Microbiology & Infectious Diseases: Official Publication of the European Society of Clinical Microbiology"},{"key":"pntd.0011335.ref031","first-page":"2021","article-title":"Contribution of the Toxoplasma ICT IgG IgM test in determining the immune status of pregnant women against toxoplasmosis","volume":"35","author":"R Ben-Abdallah","year":"2021","journal-title":"J Laboratory Clinical Analysis"},{"issue":"3","key":"pntd.0011335.ref032","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1007\/s40124-022-00269-w","article-title":"Building Programs to Eradicate Toxoplasmosis Part I: Introduction and Overview","volume":"10","author":"MS Felin","year":"2022","journal-title":"Curr Pediatr Rep"},{"issue":"3","key":"pntd.0011335.ref033","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1007\/s40124-022-00267-y","article-title":"Building Programs to Eradicate Toxoplasmosis Part II: Education","volume":"10","author":"MS Felin","year":"2022","journal-title":"Curr Pediatr Rep"},{"issue":"3","key":"pntd.0011335.ref034","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1007\/s40124-022-00265-0","article-title":"Building Programs to Eradicate Toxoplasmosis Part III: Epidemiology and Risk Factors","volume":"10","author":"MS Felin","year":"2022","journal-title":"Curr Pediatr Rep"},{"issue":"3","key":"pntd.0011335.ref035","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1007\/s40124-022-00268-x","article-title":"Building Programs to Eradicate Toxoplasmosis Part IV: Understanding and Development of Public Health Strategies and Advances \"Take a Village\"","volume":"10","author":"MS Felin","year":"2022","journal-title":"Curr Pediatr Rep"},{"issue":"6","key":"pntd.0011335.ref036","first-page":"764","volume":"10","author":"C Flores","year":"2021","journal-title":"Prevalence and risk factors associated with T. gondii infection in pregnant women and newborns from Panama Pathogens"},{"issue":"3","key":"pntd.0011335.ref037","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.diagmicrobio.2016.09.001","article-title":"Validation of IgG, IgM multiplex plasmonic gold platform in French clinical cohorts for the serodiagnosis and follow-up of Toxoplasma gondii infection","volume":"87","author":"C Pomares","year":"2017","journal-title":"Diagn Microbiol Infect Dis"},{"key":"pntd.0011335.ref038","unstructured":"Public Health Service, Department of Health and Human Services (US), Food and Drug Administration. (1997). FDA public health advisory: limitations of toxoplasmosis IgM commercial test kits (letter)Washington Department of Health and Human Services (US)"},{"key":"pntd.0011335.ref039","article-title":"Submission to the Committee on Public Health Illinois State Senate","volume":"13","author":"R. McLeod","year":"2010","journal-title":"Regarding: Prenatal and Neonatal Congenital Toxoplasmosis Prevention and Treatment Act, SB3667 in the context of the National Collaborative Chicago Based Congenital Toxoplasmosis Study"},{"issue":"7","key":"pntd.0011335.ref040","doi-asserted-by":"crossref","first-page":"2334","DOI":"10.1128\/JCM.00182-08","article-title":"LDBio-Toxo II Immunoglobulin G Western Blot Confirmatory Test for Anti-T Toxoplasma Antibody Detection","volume":"46","author":"J Franck","year":"2008","journal-title":"J Clin Microbiol"},{"issue":"9","key":"pntd.0011335.ref041","doi-asserted-by":"crossref","first-page":"1322","DOI":"10.1128\/CVI.00128-09","article-title":"Bicentric Evaluation of Six Anti-Toxoplasma Immunoglobulin G (IgG) Automated Immunoassays and Comparison to the Toxo II IgG Western Blot","volume":"16","author":"A Maudry","year":"2009","journal-title":"Clin Vaccine Immunol"},{"issue":"11","key":"pntd.0011335.ref042","doi-asserted-by":"crossref","first-page":"1908","DOI":"10.1128\/CVI.05303-11","article-title":"Utility of immunoblotting for early diagnosis of t. oxoplasmosis seroconversion in pregnant women","volume":"18","author":"C Jost","year":"2011","journal-title":"Clin Vaccine Immunol CVI"},{"issue":"1","key":"pntd.0011335.ref043","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.diagmicrobio.2015.09.009","article-title":"Serological diagnosis of Toxoplasma gondii infection: Recommendations from the French National Reference Center for Toxoplasmosis","volume":"84","author":"O Villard","year":"2016","journal-title":"Diagn Microbiol Infect Dis"},{"key":"pntd.0011335.ref044","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1051\/parasite\/2019052","article-title":"Performance of seven commercial automated assays for the detection of low levels of anti-Toxoplasma IgG in French immunocompromised patients","volume":"26","author":"T Douet","year":"2019","journal-title":"Parasite"},{"key":"pntd.0011335.ref045","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1051\/parasite\/2020006","article-title":"Serological diagnosis of Toxoplasma gondii: analysis of false-positive IgG results and implications","volume":"27","author":"L Simon","year":"2020","journal-title":"Parasite Paris Fr"},{"issue":"11","key":"pntd.0011335.ref046","first-page":"1081","volume":"53","author":"K Boyer","year":"2011","journal-title":"Unrecognized Ingestion of Toxoplasma gondiiOocysts Leads to Congenital Toxoplasmosis and Causes Epidemics in North America Clin Infect Dis"},{"issue":"1","key":"pntd.0011335.ref047","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.jiph.2022.11.023","article-title":"Evaluation of the acceptability of point of care diagnostic test for prenatal toxoplasmosis (translational research phase III)","volume":"16","author":"JC Londo\u00f1o-Martinez","year":"2022","journal-title":"J Infect Public Health"},{"key":"pntd.0011335.ref048","author":"M Mejia-Oquendo","year":"2021","journal-title":"Evaluation of the impact of the first evidence-based guidelines for congenital toxoplasmosis in Armenia (Quind\u00edo) Colombia: An observational retrospective analysis-NC-ND license"},{"key":"pntd.0011335.ref049","doi-asserted-by":"crossref","first-page":"115733","DOI":"10.1016\/j.diagmicrobio.2022.115733","article-title":"Frequency of natural antibodies and concordance analysis for anti-TOXOPLASMA IgM tests in Colombian sera of pregnant women","volume":"103","author":"MI Plazas","year":"2022","journal-title":"Diagnostic Microbiology and Infectious Disease"},{"key":"pntd.0011335.ref050","article-title":"Evaluating a toxoplasma IgG avidity ELISA test for diagnostic purposes during pregnancy and correlating it with toxoplasma IgM and IgA in the Biomedical Research Centre\u2019s laboratory at the Universidad del Quind\u00edo, 2008","volume":"59","author":"E Torres-Morales","year":"2008","journal-title":"Revista Colombiana de Obstetricia y Ginecologia"},{"key":"pntd.0011335.ref051","doi-asserted-by":"crossref","first-page":"203","DOI":"10.3389\/fcimb.2020.00203","article-title":"Potent Tetrahydroquinolone Eliminates Apicomplexan Parasites","volume":"10","author":"MJ McPhillie","year":"2020","journal-title":"Front Cell Infect Microbiol"},{"issue":"1","key":"pntd.0011335.ref052","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-020-73210-0","article-title":"Engineering and characterization of a novel Self Assembling Protein for Toxoplasma peptide vaccine in HLA-A*11:01, HLA-A*02:01 and HLA-B*07:02 transgenic mice","volume":"10","author":"K El Bissati","year":"2020","journal-title":"Sci Rep"},{"issue":"3","key":"pntd.0011335.ref053","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1645\/22-72","article-title":"Use of point of care test to determine the prevalence of antibodies to Toxoplasma gondii in black bears from North Carolina and Pennsylvania","volume":"109","author":"J Strules","year":"2023","journal-title":"J Parasitol"},{"key":"pntd.0011335.ref054","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.ijppaw.2023.09.002","article-title":"Nasal vaccination of six squirrel monkeys (Saimiri sciureus): Improved immunization protocol against Toxoplasma gondii with a nanoparticle-born vaccine","volume":"22","author":"F Fasquelle","year":"2023","journal-title":"International Journal for Parasitology: Parasites and Wildlife International Journal for Parasitology: Parasites and Wildlife"}],"container-title":["PLOS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0011335","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,28]],"date-time":"2024-05-28T18:39:25Z","timestamp":1716921565000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0011335"}},"subtitle":[],"editor":[{"given":"Luisa","family":"Magalh\u00e3es","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2024,5,28]]},"references-count":54,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2024,5,28]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0011335","relation":{},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,28]]}}}