{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T20:16:36Z","timestamp":1782332196359,"version":"3.54.5"},"update-to":[{"DOI":"10.1371\/journal.pntd.0011411","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2023,7,7]],"date-time":"2023-07-07T00:00:00Z","timestamp":1688688000000}}],"reference-count":66,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2023,6,26]],"date-time":"2023-06-26T00:00:00Z","timestamp":1687737600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82072114"],"award-info":[{"award-number":["82072114"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Natural Science Foundation of Changsha","award":["kq2202129"],"award-info":[{"award-number":["kq2202129"]}]},{"DOI":"10.13039\/501100004761","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"crossref","award":["2022JJ40671"],"award-info":[{"award-number":["2022JJ40671"]}],"id":[{"id":"10.13039\/501100004761","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004543","name":"China Scholarship Council","doi-asserted-by":"publisher","award":["202206370160"],"award-info":[{"award-number":["202206370160"]}],"id":[{"id":"10.13039\/501100004543","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Background<\/jats:title>\n<jats:p>The flesh fly, <jats:italic>Sarcophaga peregrina<\/jats:italic> (Diptera: Sarcophagidae), is an important hygiene pest, that causes myiasis in humans and other mammals, typically livestock, and as a vector for various parasitic agents, including bacteria, viruses, and parasites. The role of long non-coding RNAs (lncRNAs) in regulating gene expression during metamorphosis of the flesh fly has not been well established.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methodology\/Principal findings<\/jats:title>\n<jats:p>In this study, we performed genome-wide identification and characterization of lncRNAs from the early pupal stage (1-days pupae), mid-term pupal stage (5-days pupae), and late pupal stage (9-days pupae) of <jats:italic>S<\/jats:italic>. <jats:italic>peregrina<\/jats:italic> by RNA-seq, and a total of 6921 lncRNAs transcripts were identified. RT-qPCR and enrichment analyses revealed the differentially expressed lncRNAs (DE lncRNAs) that might be associated with insect metamorphosis development. Furthermore, functional analysis revealed that the DE lncRNA (<jats:italic>SP_lnc5000<\/jats:italic>) could potentially be involved in regulating the metamorphosis of <jats:italic>S<\/jats:italic>. <jats:italic>peregrina<\/jats:italic>. RNA interference of <jats:italic>SP_lnc5000<\/jats:italic> caused reduced expression of metamorphosis-related genes in 20-hydroxyecdysone (20E) signaling (Br-c, Ftz-F1), cuticle tanning pathway (TH, DOPA), and chitin related pathway (Cht5). Injection of ds<jats:italic>SP_lnc5000<\/jats:italic> in 3rd instar larvae of <jats:italic>S<\/jats:italic>. <jats:italic>peregrina<\/jats:italic> resulted in deformed pupae, stagnation of pupal-adult metamorphosis, and a decrease in development time of pupal, pupariation rates and eclosion rates. Hematoxylin-eosin staining (H&amp;E), scanning electron microscope (SEM) observation and cuticle hydrocarbons (CHCs) analysis indicated that <jats:italic>SP_lnc5000<\/jats:italic> had crucial roles in the metamorphosis developmental by modulating pupal cuticular development.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Conclusions\/Significance<\/jats:title>\n<jats:p>We established that the lncRNA <jats:italic>SP_lnc5000<\/jats:italic> potentially regulates the metamorphosis of <jats:italic>S<\/jats:italic>. <jats:italic>peregrina<\/jats:italic> by putatively affecting the structure and composition of the pupal cuticle. This study enhances our understanding of lncRNAs as regulators of metamorphosis in <jats:italic>S<\/jats:italic>. <jats:italic>peregrina<\/jats:italic>, and provide valuable insights into the identification of potential targets for vector control and the development of effective strategies for controlling the spread of myiasis and parasitic diseases.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pntd.0011411","type":"journal-article","created":{"date-parts":[[2023,6,26]],"date-time":"2023-06-26T17:28:53Z","timestamp":1687800533000},"page":"e0011411","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":10,"title":["Genome-wide analysis of long noncoding RNAs and their association in regulating the metamorphosis of the Sarcophaga peregrina (Diptera: Sarcophagidae)"],"prefix":"10.1371","volume":"17","author":[{"given":"Yanjie","family":"Shang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yakai","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lipin","family":"Ren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangyan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengqin","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changquan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7468-8061","authenticated-orcid":true,"given":"Yadong","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,6,26]]},"reference":[{"key":"pntd.0011411.ref001","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.ibmb.2017.09.003","article-title":"Identification 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