{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T20:15:43Z","timestamp":1791490543978,"version":"4.3.4"},"update-to":[{"DOI":"10.1371\/journal.pntd.0011481","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2023,7,27]],"date-time":"2023-07-27T00:00:00Z","timestamp":1690416000000}}],"reference-count":56,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2023,7,17]],"date-time":"2023-07-17T00:00:00Z","timestamp":1689552000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32002245"],"award-info":[{"award-number":["32002245"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31760020"],"award-info":[{"award-number":["31760020"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U1803236"],"award-info":[{"award-number":["U1803236"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>Iron is an essential element required for all organisms. Iron response regulator (Irr) is a crucial transcriptional regulator and can affect the growth and iron uptake of <jats:italic>Brucella<\/jats:italic>. The growth rate of <jats:italic>Brucella melitensis<\/jats:italic> M5-90 <jats:italic>irr<\/jats:italic> mutant was significantly lower than that of <jats:italic>B<\/jats:italic>. <jats:italic>melitensis<\/jats:italic> M5-90 under normal or iron-sufficient conditions, however, the growth rate of the <jats:italic>B<\/jats:italic>. <jats:italic>melitensis<\/jats:italic> M5-90 <jats:italic>irr<\/jats:italic> mutant was significantly higher than that of <jats:italic>B<\/jats:italic>. <jats:italic>melitensis<\/jats:italic> M5-90 under iron-limited conditions. In addition, <jats:italic>irr<\/jats:italic> mutation significantly reduced iron uptake under iron-limited conditions. Previous studies suggested that the Irr protein has multiple target genes in the <jats:italic>Brucella<\/jats:italic> genome that are involved in iron metabolism. Therefore, in the present study, a Dap-seq approach was used to investigate the other iron metabolism genes that are also regulated by the Irr protein in <jats:italic>Brucella<\/jats:italic>. A total of seven genes were identified as target genes for Irr in this study and the expression levels of these seven genes was identified using qRT-PCR. The electrophoretic mobility shift assay confirmed that six out of the seven genes, namely <jats:italic>rirA<\/jats:italic> (BME_RS13665), <jats:italic>membrane protein<\/jats:italic> (BME_RS01725), <jats:italic>hypothetical protein<\/jats:italic> (BME_RS09560), <jats:italic>ftrA<\/jats:italic> (BME_RS14525), <jats:italic>cation-transporting P-type ATPase (zntA)<\/jats:italic> (BME_RS10660), and <jats:italic>2Fe-2S binding protein<\/jats:italic> (BME_RS13655), interact with the Irr protein. Furthermore, the iron utilization and growth assay experiments confirmed that <jats:italic>rirA<\/jats:italic> was involve in iron metabolism and growth of <jats:italic>Brucella<\/jats:italic>. In summary, our results identified six genes regulated by the Irr protein that may participate in iron metabolism, and the <jats:italic>rirA<\/jats:italic> was identified as a regulon of Irr and it also plays a role in iron metabolism of <jats:italic>Brucella<\/jats:italic>. Collectively, these results provide valuable insights for the exploration of <jats:italic>Brucella<\/jats:italic> iron metabolism.<\/jats:p>","DOI":"10.1371\/journal.pntd.0011481","type":"journal-article","created":{"date-parts":[[2023,7,17]],"date-time":"2023-07-17T17:32:35Z","timestamp":1689615155000},"page":"e0011481","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":10,"title":["Scanning iron response regulator binding sites using Dap-seq in the Brucella genome"],"prefix":"10.1371","volume":"17","author":[{"given":"Huan","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianhao","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xudong","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongchen","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yueli","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ningning","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingguo","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyu","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honghuan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benben","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jihai","family":"Yi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5181-6518","authenticated-orcid":true,"given":"Chuangfu","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,7,17]]},"reference":[{"key":"pntd.0011481.ref001","first-page":"5","article-title":"Brucellosis: A Highly Infectious Zoonosis of Public Health and Economic Importance","volume":"3","author":"G Berhanu","year":"2020","journal-title":"Journal of Emerging Environmental Technologies and Health Protection"},{"key":"pntd.0011481.ref002","doi-asserted-by":"crossref","DOI":"10.3201\/eid0302.970219","article-title":"Brucellosis: An Overview.","author":"Corbel","year":"1997","journal-title":"Emerging Infectious Diseases"},{"issue":"supp-S1","key":"pntd.0011481.ref003","article-title":"The Changing Brucella Ecology: Novel Reservoirs, New Threats","volume":"36","author":"G. 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