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The activation of hepatic stellate cells (HSCs) is a key event in the development of liver fibrosis. However, the molecular mechanism of HSC activation in the<jats:italic>E<\/jats:italic>.<jats:italic>multilocularis<\/jats:italic>infection-induced liver fibrosis remains largely unclear. Here, we reported that mmu-miR-342-3p was most dominantly expressed in HSCs and was upregulated in the HSCs in response to<jats:italic>E<\/jats:italic>.<jats:italic>multilocularis<\/jats:italic>infection. We further showed that mmu-miR-342-3p was able to bind to the 3\u2019 UTR of the<jats:italic>Zbtb7a<\/jats:italic>gene and regulated its expression. Moreover, mmu-miR-342-3p expression was negatively correlated with its target gene<jats:italic>Zbtb7a<\/jats:italic>in HSCs during<jats:italic>E<\/jats:italic>.<jats:italic>multilocularis<\/jats:italic>infection. Knockdown of mmu-miR-342-3p promoted the expression of<jats:italic>Gfap<\/jats:italic>in the activated HSCs<jats:italic>in vitro<\/jats:italic>. In the<jats:italic>E<\/jats:italic>.<jats:italic>multilocularis<\/jats:italic>-infected mice, knockdown of mmu-miR-342-3p suppressed the expression of<jats:italic>\u03b1-Sma<\/jats:italic>,<jats:italic>Col1\u03b11<\/jats:italic>, and<jats:italic>TGF-\u03b2<\/jats:italic>but promoted the expression of<jats:italic>Gfap<\/jats:italic>. Therefore, mmu-miR-342-3p is a key regulator for activation of HSCs, and inhibiting mmu-miR-342-3p to suppressed Zbtb7a-mediated TGF-\u03b2 signaling in activated HSCs could be a novel strategy to treat liver fibrosis induced by<jats:italic>E<\/jats:italic>.<jats:italic>multilocularis<\/jats:italic>.<\/jats:p>","DOI":"10.1371\/journal.pntd.0011520","type":"journal-article","created":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T17:39:16Z","timestamp":1690306756000},"page":"e0011520","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":6,"title":["mmu-miRNA-342-3p promotes hepatic stellate cell activation and hepatic fibrosis induced by Echinococcus multilocularis infection via targeting Zbtb7a"],"prefix":"10.1371","volume":"17","author":[{"given":"Shanling","family":"Cao","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dexian","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yixuan","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junmei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lixia","family":"Pu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuenong","family":"Luo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueyong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaolin","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yadong","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuai","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4251-374X","authenticated-orcid":true,"given":"Xiaola","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2023,7,25]]},"reference":[{"key":"pntd.0011520.ref001","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1016\/j.pt.2019.05.005","article-title":"Echinococcus multilocularis.","volume":"35","author":"A Casulli","year":"2019","journal-title":"Trends Parasitol"},{"key":"pntd.0011520.ref002","doi-asserted-by":"crossref","first-page":"e722","DOI":"10.1371\/journal.pntd.0000722","article-title":"The global burden of alveolar echinococcosis.","volume":"4","author":"PR Torgerson","year":"2010","journal-title":"PLoS Negl Trop Dis"},{"key":"pntd.0011520.ref003","article-title":"Echinococcosis: Advances in the 21st","volume":"32","author":"H Wen","year":"2019","journal-title":"Century. 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