{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T23:27:51Z","timestamp":1768692471614,"version":"3.49.0"},"update-to":[{"DOI":"10.1371\/journal.pntd.0011626","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T00:00:00Z","timestamp":1696982400000}}],"reference-count":45,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T00:00:00Z","timestamp":1695945600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"S&T Major Project of Lishui","award":["2023GYX03"],"award-info":[{"award-number":["2023GYX03"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31501050"],"award-info":[{"award-number":["31501050"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013115","name":"Zhejiang Provincial Program for the Cultivation of High-Level Innovative Health Talents","doi-asserted-by":"publisher","award":["WJW2021002"],"award-info":[{"award-number":["WJW2021002"]}],"id":[{"id":"10.13039\/501100013115","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Basic Public Welfare Research Project of Zhejiang Province","award":["LGF22H180015"],"award-info":[{"award-number":["LGF22H180015"]}]},{"name":"Medical Health Science and Technology Project of Zhejiang Provincial Health Commission","award":["2020KY530"],"award-info":[{"award-number":["2020KY530"]}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Background<\/jats:title>\n<jats:p>Artesunate (ART) has been reported to have an antifibrotic effect in various organs. The underlying mechanism has not been systematically elucidated. We aimed to clarify the effect of ART on liver fibrosis induced by <jats:italic>Schistosoma japonicum<\/jats:italic> (<jats:italic>S<\/jats:italic>. <jats:italic>japonicum<\/jats:italic>) in an experimentally infected rodent model and the potential underlying mechanisms.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methods<\/jats:title>\n<jats:p>The effect of ART on hepatic stellate cells (HSCs) was assessed using CCK-8 and Annexin V-FITC\/PI staining assays. The experimental model of liver fibrosis was established in the Mongolian gerbil model infected with <jats:italic>S<\/jats:italic>. <jats:italic>japonicum<\/jats:italic> cercariae and then treated with 20 mg\/kg or 40 mg\/kg ART. The hydroxyproline (Hyp) content, malondialdehyde (MDA) content, superoxide dismutase (SOD) and glutathione peroxidase (GPX) activities in liver tissue were measured and histopathological changes of liver tissues were observed. Whole-transcriptome RNA sequencing (RNA-seq) of the liver tissues was performed. Differentially expressed genes (DEGs) were identified using bioinformatic analysis and verified by quantitative PCR (qPCR) and western blot assay.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Results<\/jats:title>\n<jats:p>ART significantly inhibited the proliferation and induce the apoptosis of HSCs in a dose-dependent manner. <jats:italic>In vivo<\/jats:italic>, Hyp content decreased significantly in the ART-H group compared to the model (MOD) group and GPX activity was significantly higher in the ART-H group than in the MOD group. Besides, ART treatment significantly reduced collagen production (p &lt;0.05). A total of 158 DEGs and 44 differentially expressed miRNAs related to ART-induced anti-schistosomiasis liver fibrosis were identified. The qPCR and western blot results of selected DEGs were consistent with the sequencing results. These DEGs were implicated in key pathways such as immune and inflammatory response, integrin-mediated signaling and toll-like receptor signaling pathways.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec004\">\n<jats:title>Conclusion<\/jats:title>\n<jats:p>ART is effective against liver fibrosis using Mongolian gerbil model induced by <jats:italic>S<\/jats:italic>. <jats:italic>japonicum<\/jats:italic> infection. We identified host candidate regulators of schistosomiasis-induced liver fibrosis in response to ART through transcriptomics approach.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pntd.0011626","type":"journal-article","created":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T17:29:43Z","timestamp":1696008583000},"page":"e0011626","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":5,"title":["Mining host candidate regulators of schistosomiasis-induced liver fibrosis in response to artesunate therapy through transcriptomics 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Pharmacol"},{"key":"pntd.0011626.ref035","doi-asserted-by":"crossref","first-page":"36889","DOI":"10.1038\/srep36889","article-title":"DDIT4 regulates mesenchymal stem cell fate by mediating between HIF1alpha and mTOR signalling.","volume":"6","author":"B Gharibi","year":"2016","journal-title":"Sci Rep."},{"issue":"12","key":"pntd.0011626.ref036","doi-asserted-by":"crossref","first-page":"118517","DOI":"10.1016\/j.bbamcr.2019.07.012","article-title":"Lysosomal cysteine protease cathepsin S is involved in cancer cell motility by regulating store-operated Ca(2+) entry.","volume":"1866","author":"HH Lin","year":"2019","journal-title":"Biochim Biophys Acta Mol Cell Res"},{"key":"pntd.0011626.ref037","doi-asserted-by":"crossref","first-page":"516746","DOI":"10.3389\/fonc.2020.516746","article-title":"Inhibition of Cathepsin S Induces Mitochondrial Apoptosis in Glioblastoma Cell Lines Through Mitochondrial Stress and Autophagosome Accumulation.","volume":"10","author":"M Fei","year":"2020","journal-title":"Front Oncol"},{"issue":"4","key":"pntd.0011626.ref038","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1359\/jbmr.2003.18.4.669","article-title":"Leupaxin is a critical adaptor protein in the adhesion zone of the osteoclast","volume":"18","author":"A Gupta","year":"2003","journal-title":"J Bone Miner Res"},{"issue":"6","key":"pntd.0011626.ref039","doi-asserted-by":"crossref","first-page":"3611","DOI":"10.4049\/jimmunol.176.6.3611","article-title":"The gamma-parvin-integrin-linked kinase complex is critically involved in leukocyte-substrate interaction","volume":"176","author":"R Yoshimi","year":"2006","journal-title":"J Immunol"},{"issue":"7","key":"pntd.0011626.ref040","doi-asserted-by":"crossref","DOI":"10.3390\/ijms21072555","article-title":"Long-Tailed Unconventional Class I Myosins in Health and Disease.","volume":"21","author":"A Navines-Ferrer","year":"2020","journal-title":"Int J Mol Sci."},{"issue":"3","key":"pntd.0011626.ref041","doi-asserted-by":"crossref","first-page":"1901380","DOI":"10.1002\/advs.201901380","article-title":"Targeting YAP1\/LINC00152\/FSCN1 Signaling Axis Prevents the Progression of Colorectal Cancer","volume":"7","author":"C Ou","year":"2020","journal-title":"Adv Sci (Weinh)."},{"issue":"8","key":"pntd.0011626.ref042","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.1111\/j.1478-3231.2008.01826.x","article-title":"Connective tissue growth factor: a fibrogenic master switch in fibrotic liver diseases","volume":"28","author":"OA Gressner","year":"2008","journal-title":"Liver Int"},{"issue":"1","key":"pntd.0011626.ref043","first-page":"125","article-title":"Role of CTGF gene promoter methylation in the development of hepatic fibrosis","volume":"8","author":"C Shi","year":"2016","journal-title":"Am J Transl 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