{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T11:46:26Z","timestamp":1780400786379,"version":"3.54.1"},"update-to":[{"DOI":"10.1371\/journal.pntd.0011644","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T00:00:00Z","timestamp":1695859200000}}],"reference-count":32,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2023,9,18]],"date-time":"2023-09-18T00:00:00Z","timestamp":1694995200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"crossref","award":["JP21jm0510005"],"award-info":[{"award-number":["JP21jm0510005"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Background<\/jats:title>\n<jats:p>Filamentous fungi of the genus <jats:italic>Madurella<\/jats:italic> are the primary causative agents of mycetoma, a disease observed in tropical and subtropical regions. Since early diagnostics based on a morphological approach are difficult and have many shortcomings, a molecular diagnostic method suitable for rural settings is required. In this study, we developed the loop-mediated isothermal amplification (LAMP) method to present a foundational technique of the diagnosis of <jats:italic>Madurella<\/jats:italic> spp. (<jats:italic>M<\/jats:italic>. <jats:italic>mycetomatis<\/jats:italic>, <jats:italic>M<\/jats:italic>. <jats:italic>pseudomycetomatis<\/jats:italic>, <jats:italic>M<\/jats:italic>. <jats:italic>tropicana<\/jats:italic>, and <jats:italic>M<\/jats:italic>. <jats:italic>fahalii<\/jats:italic>), the common causative organisms of eumycetoma.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Principal findings<\/jats:title>\n<jats:p>We successfully designed a primer pair targeting the rDNAs of three <jats:italic>Madurella<\/jats:italic> spp. excluding <jats:italic>M<\/jats:italic>. <jats:italic>fahalii<\/jats:italic>, and detected up to 100 fg of genomic DNA extracted from isolates of <jats:italic>M<\/jats:italic>. <jats:italic>mycetomatis<\/jats:italic> and 1 pg of <jats:italic>M<\/jats:italic>. <jats:italic>pseudomycetomatis<\/jats:italic> and <jats:italic>M<\/jats:italic>. <jats:italic>tropicana<\/jats:italic>, within one hour. Second, a primer pair specific to <jats:italic>M<\/jats:italic>. <jats:italic>mycetomatis<\/jats:italic>, the most common causative species, or <jats:italic>M<\/jats:italic>. <jats:italic>fahalii<\/jats:italic>, a drug-resistant species, was constructed, and the detection limit of both primer pairs was 1 pg. The designed primers accurately distinguished 16 strains of the genus <jats:italic>Madurella<\/jats:italic> from various fungal species known to cause mycetomas.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Conclusion<\/jats:title>\n<jats:p>In summary, we established the first model of a LAMP detection method that rapidly and sensitively detects and identifies <jats:italic>Madurella<\/jats:italic> isolates for clinical diagnostics. Moreover, the combined designed primer sets could identify mycetoma-causing strains simultaneously.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pntd.0011644","type":"journal-article","created":{"date-parts":[[2023,9,18]],"date-time":"2023-09-18T17:28:09Z","timestamp":1695058089000},"page":"e0011644","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":8,"title":["Specific and sensitive loop-mediated isothermal amplification (LAMP) method for Madurella strains, eumycetoma filamentous fungi causative agent"],"prefix":"10.1371","volume":"17","author":[{"given":"Isato","family":"Yoshioka","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yugo","family":"Mori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed 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