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In Uganda, <jats:italic>Leptospira<\/jats:italic> exposure has been reported in humans, with domesticated animals being speculated as the source. However, comparable evidence of <jats:italic>Leptospira<\/jats:italic> prevalence and circulating serovars\/serogroups in animals is only documented for cattle, and dogs. Our study determined <jats:italic>Leptospira<\/jats:italic> seroprevalence, associated risk factors and serogroups circulating among slaughtered pigs, goats, and sheep in Uganda.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methods<\/jats:title>\n<jats:p>During an 11-month cross-sectional survey in selected slaughter facilities in three regions of Uganda, we collected blood from 926 pigs, 347 goats, and 116 sheep. The age, sex, breed, and origin of each sampled animal were noted. The samples were tested for anti-<jats:italic>Leptospira<\/jats:italic> antibodies using the microscopic agglutination test, based on a panel of 12 serovars belonging to 12 serogroups.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Results<\/jats:title>\n<jats:p><jats:italic>Leptospira<\/jats:italic> seroprevalence was 26.67% (247\/926, 95%CI 23.92\u201329.61) among pigs, and 21.81% (101\/463, 95%CI 18.29\u201325.80) in goats and sheep (small ruminants). <jats:italic>L<\/jats:italic>. <jats:italic>interrogans<\/jats:italic> Australis and <jats:italic>L<\/jats:italic>. <jats:italic>kirschneri<\/jats:italic> Grippotyphosa were the commonest serovars among pigs, as was <jats:italic>L<\/jats:italic>. <jats:italic>borgpetersenii<\/jats:italic> Tarassovi in small ruminants. Pigs sourced from the Eastern (Odds Ratio [OR] = 2.82, 95%CI 1.84\u20134.30) and Northern (OR = 3.56, 95%CI 2.52\u20135.02) regions were more likely to be seropositive, compared to those from the Central region. For small ruminants, being female (OR 2.74, 95% CI 1.69\u20134.57) and adult (OR 4.47, 95% CI 1.57\u201318.80) was significantly more associated with <jats:italic>Leptospira<\/jats:italic> seropositivity.<\/jats:p>\n<jats:p>Conclusion\/significance: Detection of a moderate seroprevalence, and several <jats:italic>Leptospira<\/jats:italic> serogroups among pigs, sheep, and goats from all regions of Uganda, supports existing reports in cattle and dogs, and implies widespread <jats:italic>Leptospira<\/jats:italic> exposure in domestic animals in Uganda. These findings may inform future programs for the control of leptospirosis in livestock in Uganda.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pntd.0012055","type":"journal-article","created":{"date-parts":[[2024,3,15]],"date-time":"2024-03-15T17:56:09Z","timestamp":1710525369000},"page":"e0012055","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":9,"title":["Cross-sectional serosurvey of Leptospira species among slaughter pigs, goats, and sheep in Uganda"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5432-6407","authenticated-orcid":true,"given":"Lordrick","family":"Alinaitwe","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher 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