{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T06:59:29Z","timestamp":1770965969851,"version":"3.50.1"},"update-to":[{"DOI":"10.1371\/journal.pntd.0012092","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2024,5,21]],"date-time":"2024-05-21T00:00:00Z","timestamp":1716249600000}}],"reference-count":20,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2024,4,5]],"date-time":"2024-04-05T00:00:00Z","timestamp":1712275200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010665","name":"H2020 Marie Sk\u0142odowska-Curie Actions","doi-asserted-by":"publisher","award":["707404"],"award-info":[{"award-number":["707404"]}],"id":[{"id":"10.13039\/100010665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003246","name":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["015.013.033"],"award-info":[{"award-number":["015.013.033"]}],"id":[{"id":"10.13039\/501100003246","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001828","name":"Erasmus Universiteit Rotterdam","doi-asserted-by":"publisher","award":["EUR fellowship"],"award-info":[{"award-number":["EUR fellowship"]}],"id":[{"id":"10.13039\/501100001828","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p><jats:italic>Madurella mycetomatis<\/jats:italic> is the main cause of mycetoma, a chronic granulomatous infection for which currently no adequate therapy is available. To improve therapy, more knowledge on a molecular level is required to understand how <jats:italic>M<\/jats:italic>. <jats:italic>mycetomatis<\/jats:italic> is able to cause this disease. However, the genetic toolbox for <jats:italic>M<\/jats:italic>. <jats:italic>mycetomatis<\/jats:italic> is limited. To date, no method is available to genetically modify <jats:italic>M<\/jats:italic>. <jats:italic>mycetomatis<\/jats:italic>. In this paper, a protoplast-mediated transformation protocol was successfully developed for this fungal species, using hygromycin as a selection marker. Furthermore, using this method, a cytoplasmic-GFP-expressing <jats:italic>M<\/jats:italic>. <jats:italic>mycetomatis<\/jats:italic> strain was created. The reported methodology will be invaluable to explore the pathogenicity of <jats:italic>M<\/jats:italic>. <jats:italic>mycetomatis<\/jats:italic> and to develop reporter strains which can be useful in drug discovery as well as in genetic studies.<\/jats:p>","DOI":"10.1371\/journal.pntd.0012092","type":"journal-article","created":{"date-parts":[[2024,4,5]],"date-time":"2024-04-05T16:28:46Z","timestamp":1712334526000},"page":"e0012092","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":5,"title":["Protoplast-mediated transformation of Madurella mycetomatis using hygromycin resistance as a selection marker"],"prefix":"10.1371","volume":"18","author":[{"given":"Saskia","family":"du Pr\u00e9","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3983-4304","authenticated-orcid":true,"given":"Mickey","family":"Konings","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-2445-2972","authenticated-orcid":true,"given":"Dorenda J. 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