{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T21:22:31Z","timestamp":1790025751823,"version":"4.0.1"},"update-to":[{"DOI":"10.1371\/journal.pntd.0012142","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2024,6,4]],"date-time":"2024-06-04T00:00:00Z","timestamp":1717459200000}}],"reference-count":38,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T00:00:00Z","timestamp":1715558400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100019184","name":"BiodivERsA","doi-asserted-by":"crossref","award":["ANR-19-EBI3-0009"],"award-info":[{"award-number":["ANR-19-EBI3-0009"]}],"id":[{"id":"10.13039\/100019184","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Seoul virus (SEOV) is an orthohantavirus primarily carried by rats. In humans, it may cause hemorrhagic fever with renal syndrome (HFRS). Its incidence is likely underestimated and given the expansion of urban areas, a better knowledge of SEOV circulation in rat populations is called for. Beyond the need to improve human case detection, we need to deepen our comprehension of the ecological, epidemiological, and evolutionary processes involved in the transmission of SEOV.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methodology \/ Principal findings<\/jats:title>\n                    <jats:p>\n                      We performed a comprehensive serological and molecular characterization of SEOV in\n                      <jats:italic>Rattus norvegicus<\/jats:italic>\n                      in a popular urban park within a large city (Lyon, France) to provide essential information to design surveillance strategies regarding SEOV. We sampled rats within the urban park of \u2018La T\u00eate d\u2019Or\u2019 in Lyon city from 2020 to 2022. We combined rat population genetics, immunofluorescence assays, SEOV high-throughput sequencing (S, M, and L segments), and phylogenetic analyses. We found low structuring of wild rat populations within Lyon city. Only one sampling site within the park (building created in 2021) showed high genetic differentiation and deserves further attention. We confirmed the circulation of SEOV in rats from the park with high seroprevalence (17.2%) and high genetic similarity with the strain previously described in 2011 in Lyon city.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Conclusion\/Significance<\/jats:title>\n                    <jats:p>This study confirms the continuous circulation of SEOV in a popular urban park where the risk for SEOV transmission to humans is present. Implementing a surveillance of this virus could provide an efficient early warning system and help prepare risk-based interventions. As we reveal high gene flow between rat populations from the park and the rest of the city, we advocate for SEOV surveillance to be conducted at the scale of the entire city.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pntd.0012142","type":"journal-article","created":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T13:38:44Z","timestamp":1715607524000},"page":"e0012142","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":9,"title":["Evolution and genetic characterization of Seoul virus in wild rats Rattus norvegicus from an urban park in Lyon, France 2020\u20132022"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7847-3762","authenticated-orcid":true,"given":"Hussein","family":"Alburkat","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teemu","family":"Smura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Bouilloud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julien","family":"Pradel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gwendoline","family":"Anfray","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karine","family":"Berthier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lara","family":"Dutra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Loiseau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thanakorn","family":"Niamsap","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viktor","family":"Olander","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Sepulveda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vinaya","family":"Venkat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Charbonnel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillaume","family":"Castel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tarja","family":"Sironen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,5,13]]},"reference":[{"key":"pntd.0012142.ref001","doi-asserted-by":"crossref","first-page":"e1000172","DOI":"10.1371\/journal.ppat.1000172","article-title":"Immunological mechanisms mediating hantavirus persistence in rodent reservoirs","volume":"4","author":"J.D. 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Johnson","year":"2017","journal-title":"Science"},{"issue":"1","key":"pntd.0012142.ref008","first-page":"2692","article-title":"Seoul hantavirus, and Rickettsia typhi.","volume":"12","author":"J Griffiths","year":"2022","journal-title":"Survey of rodent-borne pathogens in Singapore reveals the circulation of Leptospira spp.Sci Rep"},{"issue":"1","key":"pntd.0012142.ref009","doi-asserted-by":"crossref","first-page":"102730","DOI":"10.1016\/j.bjid.2022.102730","article-title":"First study of Seoul virus (SEOV) in urban rodents from newly urbanized areas of Gran La Plata, Argentina.","volume":"27","author":"B Fitte","year":"2023","journal-title":"Braz J Infect Dis"},{"issue":"3","key":"pntd.0012142.ref010","doi-asserted-by":"crossref","first-page":"e91654","DOI":"10.1371\/journal.pone.0091654","article-title":"The Characteristics of Wild Rat (Rattus spp.) Populations from an Inner-City Neighborhood with a Focus on Factors Critical to the Understanding of Rat-Associated Zoonoses","volume":"9","author":"CG Himsworth","year":"2014","journal-title":"PLoS ONE"},{"issue":"39","key":"pntd.0012142.ref011","article-title":"Rats and the City: Implications of Urbanization on Zoonotic Disease Risk in Southeast Asia","volume":"119","author":"Kim R. Blasdell","year":"2022","journal-title":"Proceedings of the National Academy of Sciences"},{"key":"pntd.0012142.ref012","doi-asserted-by":"crossref","first-page":"467","DOI":"10.3390\/v15020467","article-title":"Pet rats as the likely reservoir for human Seoul Orthohantavirus infection.","volume":"15","author":"E Heuser","year":"2023","journal-title":"Viruses"},{"key":"pntd.0012142.ref013","first-page":"525","article-title":"Detection of Seoul virus in wild brown rats (Rattus norvegicus) from pig farms in Northern England.","volume":"184","author":"EG Murphy","year":"2019","journal-title":"Vet. Res"},{"key":"pntd.0012142.ref014","first-page":"27215","article-title":"First evidence of Seoul hantavirus in the wild rat population in the Netherlands.","volume":"5","author":"J Verner-Carlsson","year":"2015","journal-title":"Infect. 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Herbreteau","year":"2011","journal-title":"Protocols for field and laboratory rodent studies."},{"issue":"4","key":"pntd.0012142.ref021","first-page":"575","article-title":"The weight of wild brown rats at sexual maturity.","volume":"99","author":"Davis de","year":"1949","journal-title":"The Anatomical record"},{"key":"pntd.0012142.ref022","article-title":"CBGP\u2014Small mammal Collection","author":"Centre de Biologie pour la Gestion des Populations","year":"2018","journal-title":"Recherche Data Gouv"},{"key":"pntd.0012142.ref023","first-page":"130","article-title":"Population genetic structure of black rats in an urban environment: a case study in Cotonou, Benin. Hystrix","volume":"32","author":"SA Badou","year":"2021","journal-title":"Ital. J. 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