{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T03:44:13Z","timestamp":1792035853016,"version":"4.4.0"},"reference-count":36,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2024,6,7]],"date-time":"2024-06-07T00:00:00Z","timestamp":1717718400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key Research and Developmen Program of China","award":["2018YFE0200400"],"award-info":[{"award-number":["2018YFE0200400"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U20A20135"],"award-info":[{"award-number":["U20A20135"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U21A20180"],"award-info":[{"award-number":["U21A20180"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"EVAg-Global project","award":["871029"],"award-info":[{"award-number":["871029"]}]},{"name":"Postdoctoral Fellowship Program of CPSF","award":["GZC20232933"],"award-info":[{"award-number":["GZC20232933"]}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>Severe fever with thrombocytopenia syndrome virus (SFTSV) is a novel tick-borne viral pathogen that causes severe fever with thrombocytopenia syndrome (SFTS). The disease was initially reported in central and eastern China, then later in Japan and South Korea, with a mortality rate of 13\u201330%. Currently, no vaccines or effective therapeutics are available for SFTS treatment. In this study, three monoclonal antibodies (mAbs) targeting the SFTSV envelope glycoprotein Gn were obtained using the hybridoma technique. Two mAbs recognized linear epitopes and did not neutralize SFTSV, while the mAb 40C10 can effectively neutralized SFTSV of different genotypes and also the SFTSV-related Guertu virus (GTV) and Heartland virus (HRTV) by targeting a spatial epitope of Gn. Additionally, the mAb 40C10 showed therapeutic effect in mice infected with different genotypes of SFTSV strains against death by preventing the development of lesions and by promoting virus clearance in tissues. The therapeutic effect could still be observed in mice infected with SFTSV which were administered with mAb 40C10 after infection even up to 4 days. These findings enhance our understanding of SFTSV immunogenicity and provide valuable information for designing detection methods and strategies targeting SFTSV antigens. The neutralizing mAb 40C10 possesses the potential to be further developed as a therapeutic monoclonal antibody against SFTSV and SFTSV-related viruses.<\/jats:p>","DOI":"10.1371\/journal.pntd.0012216","type":"journal-article","created":{"date-parts":[[2024,6,7]],"date-time":"2024-06-07T13:51:05Z","timestamp":1717768265000},"page":"e0012216","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":23,"title":["Identification and characterization of three monoclonal antibodies targeting the SFTSV glycoprotein and displaying a broad spectrum recognition of SFTSV-related viruses"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-6269-356X","authenticated-orcid":true,"given":"Xiaoli","family":"Wu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abulimiti","family":"Moming","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanfang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiying","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liyan","family":"Fu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Qian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Ni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sijing","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuang","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hualin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0013-5365","authenticated-orcid":true,"given":"Shu","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5385-083X","authenticated-orcid":true,"given":"Fei","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,6,7]]},"reference":[{"issue":"2","key":"pntd.0012216.ref001","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1002\/rmv.1776","article-title":"Systematic review of severe fever with thrombocytopenia syndrome: virology, epidemiology, and clinical characteristics.","volume":"24","author":"S Liu","year":"2014","journal-title":"Rev Med Virol."},{"issue":"1","key":"pntd.0012216.ref002","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1007\/s12250-016-3931-1","article-title":"Current status of severe fever with thrombocytopenia syndrome in China.","volume":"32","author":"J Zhan","year":"2017","journal-title":"Virol Sin"},{"issue":"16","key":"pntd.0012216.ref003","doi-asserted-by":"crossref","first-page":"1523","DOI":"10.1056\/NEJMoa1010095","article-title":"Fever with thrombocytopenia associated with a novel bunyavirus in China","volume":"364","author":"XJ Yu","year":"2011","journal-title":"N Engl J Med"},{"issue":"6","key":"pntd.0012216.ref004","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1093\/infdis\/jiv144","article-title":"Phylogenetic and Geographic Relationships of Severe Fever With Thrombocytopenia Syndrome Virus in China, South Korea, and Japan","volume":"212","author":"T Yoshikawa","year":"2015","journal-title":"J Infect Dis"},{"issue":"4","key":"pntd.0012216.ref005","doi-asserted-by":"crossref","first-page":"692","DOI":"10.3201\/eid2604.191011","article-title":"Severe Fever with Thrombocytopenia Syndrome, Japan, 2013\u20132017","volume":"26","author":"Y Kobayashi","year":"2020","journal-title":"Emerging Infectious Diseases"},{"issue":"7","key":"pntd.0012216.ref006","doi-asserted-by":"crossref","first-page":"1513","DOI":"10.3201\/eid2607.190611","article-title":"Serologic Evidence of Severe Fever with Thrombocytopenia Syndrome Virus and Related Viruses in Pakistan","volume":"26","author":"A Zohaib","year":"2020","journal-title":"Emerg Infect Dis"},{"issue":"5","key":"pntd.0012216.ref007","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.3201\/eid2505.181463","article-title":"Endemic Severe Fever with Thrombocytopenia Syndrome, Vietnam","volume":"25","author":"XC Tran","year":"2019","journal-title":"Emerg Infect Dis"},{"issue":"5","key":"pntd.0012216.ref008","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1089\/vbz.2020.2720","article-title":"Isolation of Severe Fever with Thrombocytopenia Syndrome Virus from Various Tick Species in Area with Human Severe Fever with Thrombocytopenia Syndrome Cases.","volume":"21","author":"Y Sato","year":"2021","journal-title":"Vector-Borne Zoonot."},{"issue":"12","key":"pntd.0012216.ref009","doi-asserted-by":"crossref","first-page":"2994","DOI":"10.3201\/eid2612.191513","article-title":"Direct Transmission of Severe Fever with Thrombocytopenia Syndrome Virus from Domestic Cat to Veterinary Personnel","volume":"26","author":"A Yamanaka","year":"2020","journal-title":"Emerg Infect Dis"},{"issue":"6","key":"pntd.0012216.ref010","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1093\/cid\/ciu434","article-title":"Heartland virus-associated death in tennessee","volume":"59","author":"A Muehlenbachs","year":"2014","journal-title":"Clin Infect Dis"},{"issue":"1","key":"pntd.0012216.ref011","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1038\/s41426-018-0093-2","article-title":"A novel tick-borne phlebovirus, closely related to severe fever with thrombocytopenia syndrome virus and Heartland virus, is a potential pathogen","volume":"7","author":"S Shen","year":"2018","journal-title":"Emerg Microbes Infect"},{"issue":"36","key":"pntd.0012216.ref012","first-page":"E7564","article-title":"Structures of phlebovirus glycoprotein Gn and identification of a neutralizing antibody epitope","volume":"114","author":"Y Wu","year":"2017","journal-title":"Proc Natl Acad Sci U S A"},{"key":"pntd.0012216.ref013","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.coviro.2018.03.005","article-title":"Current status of Severe Fever with Thrombocytopenia Syndrome vaccine development","volume":"29","author":"LM Reece","year":"2018","journal-title":"Curr Opin Virol"},{"key":"pntd.0012216.ref014","first-page":"113","article-title":"Structure of a phleboviral envelope glycoprotein reveals a consolidated model of membrane fusion","author":"BA Halldorsson S","year":"2016","journal-title":"PNAS"},{"issue":"3","key":"pntd.0012216.ref015","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/j.ttbdis.2016.12.015","article-title":"Novel sub-lineages, recombinants and reassortants of severe fever with thrombocytopenia syndrome virus.","volume":"8","author":"Q Lv","year":"2017","journal-title":"Ticks Tick Borne Dis"},{"issue":"5","key":"pntd.0012216.ref016","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1038\/s12276-021-00610-1","article-title":"Severe fever with thrombocytopenia syndrome virus: emerging novel phlebovirus and their control strategy","volume":"53","author":"MA Casel","year":"2021","journal-title":"Exp Mol Med"},{"issue":"4","key":"pntd.0012216.ref017","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1097\/COH.0000000000000800","article-title":"Divide and conquer: broadly neutralizing antibody combinations for improved HIV-1 viral coverage","volume":"18","author":"K Wagh","year":"2023","journal-title":"Curr Opin HIV AIDS"},{"issue":"11","key":"pntd.0012216.ref018","doi-asserted-by":"crossref","first-page":"e311","DOI":"10.1016\/S1473-3099(22)00311-5","article-title":"Monoclonal antibody therapies against SARS-CoV-2","volume":"22","author":"D Focosi","year":"2022","journal-title":"Lancet Infect Dis"},{"issue":"9","key":"pntd.0012216.ref019","doi-asserted-by":"crossref","first-page":"1426","DOI":"10.1128\/CVI.00222-13","article-title":"Human antibody neutralizes severe Fever with thrombocytopenia syndrome virus, an emerging hemorrhagic Fever virus","volume":"20","author":"X Guo","year":"2013","journal-title":"Clin Vaccine Immunol"},{"issue":"2","key":"pntd.0012216.ref020","doi-asserted-by":"crossref","first-page":"e1007375","DOI":"10.1371\/journal.ppat.1007375","article-title":"An anti-Gn glycoprotein antibody from a convalescent patient potently inhibits the infection of severe fever with thrombocytopenia syndrome virus.","volume":"15","author":"KH Kim","year":"2019","journal-title":"PLoS Pathog"},{"issue":"13","key":"pntd.0012216.ref021","article-title":"A single-domain antibody inhibits SFTSV and mitigates virus-induced pathogenesis in vivo","volume":"5","author":"X Wu","year":"2020","journal-title":"JCI Insight"},{"issue":"1","key":"pntd.0012216.ref022","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1007\/s12250-017-3953-3","article-title":"Isolation, characterization, and phylogenic analysis of three new severe fever with thrombocytopenia syndrome bunyavirus strains derived from Hubei Province, China.","volume":"32","author":"Y Zhang","year":"2017","journal-title":"Virol Sin"},{"issue":"5","key":"pntd.0012216.ref023","doi-asserted-by":"crossref","first-page":"958","DOI":"10.1007\/s12250-021-00359-x","article-title":"Establishment of a Reverse Genetic System of Severe Fever with Thrombocytopenia Syndrome Virus Based on a C4 Strain.","volume":"36","author":"M Xu","year":"2021","journal-title":"Virol Sin."},{"issue":"2","key":"pntd.0012216.ref024","doi-asserted-by":"crossref","first-page":"396","DOI":"10.4269\/ajtmh.17-0059","article-title":"A Cluster of Symptomatic and Asymptomatic Infections of Severe Fever with Thrombocytopenia Syndrome Caused by Person-to-Person Transmission","volume":"97","author":"D Huang","year":"2017","journal-title":"Am J Trop Med Hyg"},{"issue":"6","key":"pntd.0012216.ref025","doi-asserted-by":"crossref","first-page":"1319","DOI":"10.4269\/ajtmh.23-0073","article-title":"The Neutralizing Monoclonal Antibodies against SFTS Group Bandaviruses Suggest New Targets of Specific or Broad-Spectrum Antivirals","volume":"109","author":"L Fu","year":"2023","journal-title":"Am J Trop Med Hyg"},{"key":"pntd.0012216.ref026","article-title":"Developing and characterizing monoclonal antibodies of Guertu bandavirus nucleoprotein for developing methods of Guertu bandavirus and severe fever with thrombocytopenia syndrome virus detection","author":"J Qian","year":"2023","journal-title":"Braz J Microbiol"},{"issue":"3","key":"pntd.0012216.ref027","doi-asserted-by":"crossref","first-page":"e0248005","DOI":"10.1371\/journal.pone.0248005","article-title":"Fine mapping epitope on Glycoprotein-Gn from Severe Fever with Thrombocytopenia Syndrome Virus.","volume":"16","author":"A Moming","year":"2021","journal-title":"PLoS One"},{"issue":"3","key":"pntd.0012216.ref028","first-page":"213","volume":"33","author":"S Dai","year":"2018","journal-title":"Zika Virus Baculovirus-Expressed Virus-Like Particles Induce Neutralizing Antibodies in Mice"},{"issue":"1","key":"pntd.0012216.ref029","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1186\/s12916-022-02558-z","article-title":"Antiviral activity and mechanism of the antifungal drug, anidulafungin, suggesting its potential to promote treatment of viral diseases.","volume":"20","author":"S Shen","year":"2022","journal-title":"BMC Med"},{"issue":"1","key":"pntd.0012216.ref030","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.jcv.2011.09.031","article-title":"Early diagnosis of novel SFTS bunyavirus infection by quantitative real-time RT-PCR assay.","volume":"53","author":"Y Sun","year":"2012","journal-title":"J Clin Virol"},{"key":"pntd.0012216.ref031","doi-asserted-by":"crossref","first-page":"797189","DOI":"10.3389\/fmicb.2021.797189","article-title":"Animal Model of Severe Fever With Thrombocytopenia Syndrome Virus Infection.","volume":"12","author":"J Sun","year":"2021","journal-title":"Front Microbiol."},{"issue":"1","key":"pntd.0012216.ref032","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1038\/s41392-021-00541-3","article-title":"Clinical effect and antiviral mechanism of T-705 in treating severe fever with thrombocytopenia syndrome.","volume":"6","author":"H Li","year":"2021","journal-title":"Signal Transduct Target Ther"},{"issue":"1","key":"pntd.0012216.ref033","doi-asserted-by":"crossref","DOI":"10.1128\/mSphere.00061-15","article-title":"Efficacy of T-705 (Favipiravir) in the Treatment of Infections with Lethal Severe Fever with Thrombocytopenia Syndrome Virus.","volume":"1","author":"H Tani","year":"2016","journal-title":"mSphere."},{"issue":"4","key":"pntd.0012216.ref034","doi-asserted-by":"crossref","first-page":"731","DOI":"10.3904\/kjim.2016.109","article-title":"In vitro antiviral activity of ribavirin against severe fever with thrombocytopenia syndrome virus","volume":"32","author":"MJ Lee","year":"2017","journal-title":"Korean J Intern Med"},{"issue":"2","key":"pntd.0012216.ref035","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1007\/s12250-020-00289-0","article-title":"Novel SFTSV Phylogeny Reveals New Reassortment Events and Migration Routes.","volume":"36","author":"X Wu","year":"2021","journal-title":"Virol Sin."},{"issue":"8","key":"pntd.0012216.ref036","doi-asserted-by":"crossref","first-page":"4384","DOI":"10.1128\/JVI.02628-12","article-title":"Severe fever with thrombocytopenia virus glycoproteins are targeted by neutralizing antibodies and can use DC-SIGN as a receptor for pH-dependent entry into human and animal cell lines","volume":"87","author":"H Hofmann","year":"2013","journal-title":"J Virol"}],"container-title":["PLOS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0012216","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,7]],"date-time":"2024-06-07T13:51:37Z","timestamp":1717768297000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0012216"}},"subtitle":[],"editor":[{"given":"Manuel","family":"Schibler","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,6,7]]},"references-count":36,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2024,6,7]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0012216","relation":{},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,7]]}}}