{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T18:09:27Z","timestamp":1769882967797,"version":"3.49.0"},"update-to":[{"DOI":"10.1371\/journal.pntd.0012263","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2024,7,22]],"date-time":"2024-07-22T00:00:00Z","timestamp":1721606400000}}],"reference-count":36,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2024,6,14]],"date-time":"2024-06-14T00:00:00Z","timestamp":1718323200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01-TW011493"],"award-info":[{"award-number":["R01-TW011493"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100006510","name":"Duke University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006510","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100019977","name":"Grainger Bioinformatics Center","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100019977","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009879","name":"FACE Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009879","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>Small terrestrial mammals are major hosts of infectious agents responsible for zoonotic diseases. Astroviruses (AstVs)\u2013the cause of non-bacterial gastroenteritis mainly affecting young children\u2013have been detected in a wide array of mammalian and avian host species. However, understanding the factors that influence AstV infection within and across hosts is limited. Here, we investigated the impact of land use changes on AstVs in terrestrial small mammals in rural northeastern Madagascar. We sampled 515 small mammals, representing seven endemic and four introduced species. Twenty-two positive samples were identified, all but one of which were found in the introduced species <jats:italic>Mus musculus<\/jats:italic> and <jats:italic>Rattus rattus<\/jats:italic> (family Muridae), with a positivity rate of 7.7% (6\/78) and 5.6% (15\/266), respectively. The non-introduced rodent case was from an endemic shrew-tenrec (family Tenrecidae). We found the highest positivity rate of AstVs infection in brushy regrowth (17.5%, 7\/40) as compared to flooded rice fields (4.60%, 8\/174), secondary forest (4.1%, 3\/74), agroforest (3.6%, 1\/28), village (2.61%, 3\/115), and semi-intact forest (0%, 0\/84). A phylogenetic analysis revealed an association between AstVs and their rodent host species. None of the viruses were phylogenetically related to AstVs previously described in Malagasy bats. This study supports AstV circulation in synanthropic animals in agricultural habitats of Madagascar and highlights the need to assess the spillover risk to human populations in rural areas.<\/jats:p>","DOI":"10.1371\/journal.pntd.0012263","type":"journal-article","created":{"date-parts":[[2024,6,14]],"date-time":"2024-06-14T17:39:26Z","timestamp":1718386766000},"page":"e0012263","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":4,"title":["Astroviruses in terrestrial Malagasy mammals"],"prefix":"10.1371","volume":"18","author":[{"given":"Victoria","family":"Carcauzon","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0633-0575","authenticated-orcid":true,"given":"James 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Shastri","year":"1998","journal-title":"J Clin Microbiol"},{"key":"pntd.0012263.ref005","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.3390\/v13061127","article-title":"Intra and cross-species transmission of astroviruses.","volume":"13","author":"SN Roach","year":"2021","journal-title":"Viruses."},{"key":"pntd.0012263.ref006","doi-asserted-by":"crossref","first-page":"e27483","DOI":"10.1371\/journal.pone.0027483","article-title":"Astrovirus infection in hospitalized infants with severe combined immunodeficiency after allogeneic hematopoietic stem cell transplantation","volume":"6","author":"W Wunderli","year":"2011","journal-title":"PLOS ONE"},{"key":"pntd.0012263.ref007","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.jcv.2016.07.004","article-title":"Novel human astroviruses: Novel human diseases?","volume":"82","author":"D-L Vu","year":"2016","journal-title":"J Clin 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surrounding areas in the SAVA region of Madagascar","author":"S Cotton","year":"2018","journal-title":"Open Science Centre, University of Jyv\u00e4skyl\u00e4"},{"key":"pntd.0012263.ref020","author":"SM Goodman","year":"2021"},{"key":"pntd.0012263.ref021","author":"V Soarimalala","year":"2011"},{"key":"pntd.0012263.ref022","doi-asserted-by":"crossref","first-page":"9107","DOI":"10.1128\/JVI.00857-08","article-title":"Novel astroviruses in insectivorous bats.","volume":"82","author":"DKW Chu","year":"2008","journal-title":"J Virol"},{"key":"pntd.0012263.ref023","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1093\/sysbio\/syq010","article-title":"New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0","volume":"59","author":"S Guindon","year":"2010","journal-title":"Syst Biol"},{"key":"pntd.0012263.ref024","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1186\/1471-2148-6-29","article-title":"Assessment of methods 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Canberra"},{"key":"pntd.0012263.ref032","doi-asserted-by":"crossref","first-page":"3119","DOI":"10.1128\/aem.63.8.3119-3122.1997","article-title":"Astrovirus survival in drinking water","volume":"63","author":"FX Abad","year":"1997","journal-title":"Appl Environ Microbiol"},{"key":"pntd.0012263.ref033","doi-asserted-by":"crossref","first-page":"1048","DOI":"10.1128\/CMR.00013-14","article-title":"Human astroviruses.","volume":"27","author":"A Bosch","year":"2014","journal-title":"Clin Microbiol Rev"},{"key":"pntd.0012263.ref034","doi-asserted-by":"crossref","first-page":"2618","DOI":"10.1016\/j.watres.2008.01.018","article-title":"Infectivity and genome persistence of rotavirus and astrovirus in groundwater and surface water","volume":"42","author":"AC Espinosa","year":"2008","journal-title":"Water Res"},{"key":"pntd.0012263.ref035","doi-asserted-by":"crossref","first-page":"73","DOI":"10.2166\/wst.2001.0714","article-title":"Astrovirus detection in wastewater samples","volume":"43","author":"RM Pint\u00f3","year":"2001","journal-title":"Water Sci Technol J Int Assoc Water Pollut Res"},{"key":"pntd.0012263.ref036","doi-asserted-by":"crossref","first-page":"6978","DOI":"10.1128\/JVI.02971-14","article-title":"Ecological drivers of virus evolution: astrovirus as a case study","volume":"89","author":"IH Mendenhall","year":"2015","journal-title":"J Virol"}],"updated-by":[{"DOI":"10.1371\/journal.pntd.0012263","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2024,7,22]],"date-time":"2024-07-22T00:00:00Z","timestamp":1721606400000}}],"container-title":["PLOS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0012263","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,22]],"date-time":"2024-07-22T17:58:58Z","timestamp":1721671138000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0012263"}},"subtitle":[],"editor":[{"given":"Abdallah M.","family":"Samy","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2024,6,14]]},"references-count":36,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2024,6,14]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pntd.0012263","relation":{"new_version":[{"id-type":"doi","id":"10.1371\/journal.pntd.0012263","asserted-by":"object"}]},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,14]]}}}