{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T17:12:54Z","timestamp":1790356374150,"version":"4.1.0"},"update-to":[{"DOI":"10.1371\/journal.pntd.0012305","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2024,7,18]],"date-time":"2024-07-18T00:00:00Z","timestamp":1721260800000}}],"reference-count":41,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2024,7,8]],"date-time":"2024-07-08T00:00:00Z","timestamp":1720396800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100006492","name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R56 AI155573"],"award-info":[{"award-number":["R56 AI155573"]}],"id":[{"id":"10.13039\/100006492","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:p>As <jats:italic>Wolbachia pipientis<\/jats:italic> is more widely being released into field populations of <jats:italic>Aedes aegypti<\/jats:italic> for disease control, the ability to select the appropriate strain for differing environments is increasingly important. A previous study revealed that longer-term quiescence in the egg phase reduced the fertility of mosquitoes, especially those harboring the <jats:italic>w<\/jats:italic>AlbB <jats:italic>Wolbachia<\/jats:italic> strain. This infertility was also associated with a greater biting rate. Here, we attempt to quantify the effect of this heightened biting behavior on the transmission potential of the dengue virus using a combination of assays for fitness, probing behavior, and vector competence, allowing repeat feeding, and incorporate these effects in a model of R<jats:sub>0<\/jats:sub>. We show that <jats:italic>Wolbachia<\/jats:italic>-infected infertile mosquitoes are more interested in feeding almost immediately after an initial blood meal relative to wild type and <jats:italic>Wolbachia<\/jats:italic>-infected fertile mosquitoes and that these differences continue for up to 8 days over the period we measured. As a result, the infertile <jats:italic>Wolbachia<\/jats:italic> mosquitoes have higher virus prevalence and loads than <jats:italic>Wolbachia<\/jats:italic>-fertile mosquitoes. We saw limited evidence of <jats:italic>Wolbachia<\/jats:italic>-mediated blocking in the disseminated tissue (legs) in terms of prevalence but did see reduced viral loads. Using a previously published estimate of the extrinsic incubation period, we demonstrate that the effect of repeat feeding\/infertility is insufficient to overcome the effects of <jats:italic>Wolbachia<\/jats:italic>-mediated blocking on R<jats:sub>0<\/jats:sub>. These estimates are very conservative, however, and we posit that future studies should empirically measure EIP under a repeat feeding model. Our findings echo previous work where periods of extensive egg quiescence affected the reproductive success of <jats:italic>Wolbachia<\/jats:italic>-infected <jats:italic>Ae<\/jats:italic>. <jats:italic>aegypti<\/jats:italic>. Additionally, we show that increased biting behavior in association with this infertility in <jats:italic>Wolbachia<\/jats:italic>-infected mosquitoes may drive greater vector competence. These relationships require further exploration, given their ability to affect the success of field releases of <jats:italic>Wolbachia<\/jats:italic> for human disease reduction in drier climates where longer egg quiescence periods are expected.<\/jats:p>","DOI":"10.1371\/journal.pntd.0012305","type":"journal-article","created":{"date-parts":[[2024,7,8]],"date-time":"2024-07-08T19:06:51Z","timestamp":1720465611000},"page":"e0012305","update-policy":"https:\/\/doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["The effect of repeat feeding on dengue virus transmission potential in Wolbachia-infected Aedes aegypti following extended egg quiescence"],"prefix":"10.1371","volume":"18","author":[{"given":"Meng-Jia","family":"Lau","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9s 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